首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   68244篇
  免费   303篇
  国内免费   558篇
系统科学   1266篇
丛书文集   544篇
教育与普及   284篇
理论与方法论   534篇
现状及发展   35480篇
研究方法   1496篇
综合类   27311篇
自然研究   2190篇
  2013年   821篇
  2012年   824篇
  2011年   2945篇
  2009年   630篇
  2008年   1019篇
  2007年   1237篇
  2006年   1240篇
  2005年   1453篇
  2004年   2617篇
  2003年   2145篇
  2002年   1910篇
  2001年   1888篇
  2000年   1389篇
  1999年   1266篇
  1998年   647篇
  1997年   782篇
  1994年   699篇
  1993年   697篇
  1992年   1202篇
  1991年   989篇
  1990年   1063篇
  1989年   872篇
  1988年   846篇
  1987年   877篇
  1986年   929篇
  1985年   1125篇
  1984年   929篇
  1983年   808篇
  1982年   874篇
  1981年   908篇
  1980年   1000篇
  1979年   1781篇
  1978年   1541篇
  1977年   1490篇
  1976年   1312篇
  1975年   1304篇
  1974年   1356篇
  1973年   1454篇
  1972年   1564篇
  1971年   1649篇
  1970年   1946篇
  1969年   1618篇
  1968年   1565篇
  1967年   1482篇
  1966年   1295篇
  1965年   1010篇
  1959年   571篇
  1958年   885篇
  1957年   673篇
  1956年   558篇
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
111.
考察了分析结构、结构构件以及技术系统中的位移、应变与应力的测量和实验方法在目前和未来的作用。现在,比以往任何时候都更有理由和有更广阔的领域需要用到实验力学方法;许多先进、现代化技术,新型测量设备,测量和赋值过程自动化系统可供使用;以先进的测量技术和计算机技术相结合而形成的各种杂交法已被提出来。利用这些方法,可以优化结构和产品,节约能源和原材料,提高产品质量,增强结构、技术系统的可靠、安全性,控制生产过程以及监督操作系统的安全性;利用这些方法,能够并且应当使技术系统可能发生的危险以及对环境的毁损降至最低程度。  相似文献   
112.
DNA fingerprinting transforms the art of cell authentication.   总被引:4,自引:0,他引:4  
G N Stacey  B J Bolton  A Doyle 《Nature》1992,357(6375):261-262
The increasing diversity of new cell cultures is seriously stretching the capabilities of traditional methods of identification. DNA fingerprinting is set to play an important role in increasing confidence in the authenticity of cultures in research and industry.  相似文献   
113.
We recently reported on a linkage study within a Quarter Horse lineage segregating hyperkalaemic periodic paralysis (HYPP), an autosomal dominant condition showing potassium-induced attacks of skeletal muscle paralysis. HYPP co-segregated with the equine adult skeletal muscle sodium channel alpha subunit gene, the same gene that causes human HYPP. We now describe the Phe to Leu mutation in transmembrane domain IVS3 which courses the horse disease. This represents the first application of molecular genetics to an important horse disease, and the data will provide an opportunity for control or eradication of this condition.  相似文献   
114.
对新球虫灵,喹乙醇,免健宝Ⅰ号,Ⅱ号配套复合饲料添加剂进行的幼兔饲养对比试验结果表明:以免健宝Ⅰ、Ⅱ号配套复合饲料添加剂联合效果为最佳,其它依次为单用兔健宝Ⅱ号、兔健宝Ⅰ号、喹乙醇,新球虫灵。而且兔健宝Ⅰ、Ⅱ号配套复合饲料添加剂有易混匀,简化配合饲料工序、节省饲料及增重快等优点。  相似文献   
115.
Thyroid cancer after Chernobyl.   总被引:17,自引:0,他引:17  
  相似文献   
116.
Transforming growth factor-beta 1 (TGF-beta 1) is a multifunctional growth factor that has profound regulatory effects on many developmental and physiological processes. Disruption of the TGF-beta 1 gene by homologous recombination in murine embryonic stem cells enables mice to be generated that carry the disrupted allele. Animals homozygous for the mutated TGF-beta 1 allele show no gross developmental abnormalities, but about 20 days after birth they succumb to a wasting syndrome accompanied by a multifocal, mixed inflammatory cell response and tissue necrosis, leading to organ failure and death. TGF-beta 1-deficient mice may be valuable models for human immune and inflammatory disorders, including autoimmune diseases, transplant rejection and graft versus host reactions.  相似文献   
117.
118.
E H Joe  K Angelides 《Nature》1992,356(6367):333-335
In myelinated nerves, segregation of voltage-dependent sodium channels to nodes of Ranvier is crucial for saltatory conduction along axons. As sodium channels associate and colocalize with ankyrin at nodes of Ranvier, one possibility is that sodium channels are recruited and immobilized at axonal sites which are specified by the subaxolemmal cytoskeleton, independent of glial cell contact. Alternatively, segregation of channels at distinct sites along the axon may depend on glial cell contact. To resolve this question, we have examined the distribution of sodium channels, ankyrin and spectrin in myelination-competent cocultures of sensory neurons and Schwann cells by immunofluorescence, using sodium channel-, ankyrin- and spectrin-specific antibodies. In the absence of Schwann cells, sodium channels, ankyrin and spectrin are homogeneously distributed on sensory axons. When Schwann cells are introduced into these cultures, the distribution of sodium channels dramatically changes so that channel clusters on axons are abundant, but ankyrin and spectrin remain homogeneously distributed. Addition of latex beads or Schwann cell membranes does not induce channel clustering. Our results suggest that segregation of sodium channels on axons is highly dependent on interactions with active Schwann cells and that continuing axon-glial interactions are necessary to organize and maintain channel distribution during differentiation of myelinated axons.  相似文献   
119.
Short alanine peptides, containing 16 or 17 residues, appear to form alpha-helices in aqueous solution. But the main spectroscopic analyses used on helical peptides (circular dichroism and nuclear magnetic resonance) cannot distinguish between an alpha-helix (in which the ith residue is hydrogen-bonded to residue i + 4; ref. 9) and the next most common peptide helix, the 3(10)-helix10 (i-->i + 3 hydrogen-bonding). To address this problem we have designed single and doubly spin-labelled analogues of alanine-based peptides in which the nitroxide spin label forms an unbranched side chain extending from the sulphur atom of a cysteine residue. Here we report the circular dichroism, Fourier-transform infrared and electron-spin resonance spectra of these peptides under helix-forming conditions. The infrared absorbance gives an amide I' band with a frequency that is substantially different from that observed for alpha-helices. The electron-spin resonance spectra of doubly labelled helices show that the ranking of distances between side chains, around a single turn (residues 4-8), is inconsistent with an alpha-helical structure. Our experiments suggest that the more likely peptide geometry is a 3(10)-helix.  相似文献   
120.
本文研究了在不同pH值下吐温—80.Tritonx—100.SLS三种表面活性剂四—(对-三甲铵苯基)卟啉Soret带的影响。  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号