Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A

We have cloned and sequenced the complete complementary DNA of the oestrogen receptor (ER) present in the breast cancer cell line MCF-7. The expression of the ER cDNA in HeLa cells produces a protein that has the same relative molecular mass and binds oestradiol with the same affinity as the MCF-7 ER. There is extensive homology between the ER and the erb-A protein of the oncogenic avian erythroblastosis virus.     

Competitive inhibitors of neurohypophyseal hormones on adenylate cyclase from the toad urinary bladder

Zusammenfassung Adenylat-Cyclase in plasmamembranreichen Präparationen des Epitheliums von der Harnblase der Kröte wird durch Arginine-Vasopressin aktiviert. Diese Aktivierung wird durch mehre Oxytocinanaloge, die alle eine Substitution des Glutaminrestes in Position 4 des Hormones durch Leucin oder Isoleucin gemeinsam haben, kompetitiv gehemmt. Der stärkste Antagonist der Serie ist [2,4-diisoleucin]-Oxytocin und die antagonistische Aktivität nimmt in der Reihenfolge [4-Leucin]-, [2-Isoleucin-4-leucin]-, [2,4-Dileucin]- und [2-Phenylalanin-4-leucin]-Oxytocin ab. Die Resultate werden an Hand der Oxytocinkonformation diskutiert.

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Supported by United States Public Health Service grants No. AM-13567 (RW), by the Life Sciences Foundation, Inc., and by the National Science Foundation No. GB-30716X (VJH).

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Neurohypophyseal hormone analogs are denoted in accordance with the IUPAC-IUB [Tentative Rules (Biochemistry6, 362, 1967)].     

Position of the methionine residue in bovine neurophysin II

Zusammenfassung Durch Spaltung mit CNBr wurde gezeigt, dass Neurophysin II aus Rinderhypophyse nur eine Methionin-Einheit enthält und dass die N-terminale Sequenz Ala-Met ist.

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This work was supported by NIH Grants No. AM-13567, No. AM-10080, by NSF Grant No. GB-17046 and by the City University of New York. The authors are indebted to Dr.L. Abrash for helpful discussion and MissM. Wahrenburg and Mr.V. S. Sapirstein for the assays.

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Predoctoral fellow of the Department of Physiology.     

Relativity of motion: From Occam to Galileo

Early observations of the southern celestial sky were reported in many sixteenth-century books and compilations of voyages of discovery. Here we analyse these accounts in order to find out what was really seen and reported by the first navigators. Our analysis had resulted in new interpretations of the phenomena reported by Amerigo Vespucci and Andreas Corsali. Thus, a reassessment of the discovery of the Coalsack Nebula, the Magellanic Clouds, and the Southern Cross can be made. From a comparative review of the observations of the latter constellation as published between 1500 and 1600, we demonstrate that only questionable records found their way to contemporary compilations of voyages of discovery, and that as a result public knowledge about this constellation at the end of the sixteenth century was entirely unreliable. Another problem we discuss is that although the stars of the Southern Cross were the first to be discovered, and were observed again and again by many navigators, it was not until 1678 that their proper positions were found in stellar atlases and star catalogues accessible to astronomers. We explain how negligence of and subsequently confidence in Ptolemy's astronomy by, respectively, the early navigators and cartographers, were at the root of this amazingly long-lasting gap in the knowledge of the southern celestial sky.     

Bimodal expression of Sprouty2 during the cell cycle is mediated by phase-specific Ras/MAPK and c-Cbl activities

Sprouty2 is an important inhibitor of cell proliferation and signal transduction. In this study, we found a bimodal expression of Sprouty2 protein during cell cycle progression after exit from quiescence, whereas elevated Sprouty4 expression in the G1 phase stayed high throughout the rest of the cell cycle. Induction of the mitogen-activated protein kinase via activated Ras was crucial for increased Sprouty2 expression at the G0/G1 transition. Following the first peak, accelerated proteasomal protein degradation caused a transient attenuation of Sprouty2 abundance during late G1. Since the decline in its expression was abolished by dominant negative c-Cbl and the timely restricted interaction between Sprouty2 and c-Cbl disappeared at the second peak of Sprouty2 expression, we conclude that the second phase in the cell cycle-specific expression profile of Sprouty2 is solely dependent on ubiquitination by c-Cbl. Our results suggest that Sprouty2 abundance is the result of strictly coordinated activities of Ras and c-Cbl.     

Stromatolite reef from the Early Archaean era of Australia

The 3,430-million-year-old Strelley Pool Chert (SPC) (Pilbara Craton, Australia) is a sedimentary rock formation containing laminated structures of probable biological origin (stromatolites). Determining the biogenicity of such ancient fossils is the subject of ongoing debate. However, many obstacles to interpretation of the fossils are overcome in the SPC because of the broad extent, excellent preservation and morphological variety of its stromatolitic outcrops--which provide comprehensive palaeontological information on a scale exceeding other rocks of such age. Here we present a multi-kilometre-scale palaeontological and palaeoenvironmental study of the SPC, in which we identify seven stromatolite morphotypes--many previously undiscovered--in different parts of a peritidal carbonate platform. We undertake the first morphotype-specific analysis of the structures within their palaeoenvironment and refute contemporary abiogenic hypotheses for their formation. Finally, we argue that the diversity, complexity and environmental associations of the stromatolites describe patterns that--in similar settings throughout Earth's history--reflect the presence of organisms.     

Rapid X-ray flaring from the direction of the supermassive black hole at the Galactic Centre.

The nuclei of most galaxies are now believed to harbour supermassive black holes. The motions of stars in the central few light years of our Milky Way Galaxy indicate the presence of a dark object with a mass of about 2.6 x 106 solar masses (refs 2, 3). This object is spatially coincident with the compact radio source Sagittarius A* (Sgr A*) at the dynamical centre of the Galaxy, and the radio emission is thought to be powered by the gravitational potential energy released by matter as it accretes onto a supermassive black hole. Sgr A* is, however, much fainter than expected at all wavelengths, especially in X-rays, which has cast some doubt on this model. The first strong evidence for X-ray emission was found only recently. Here we report the discovery of rapid X-ray flaring from the direction of Sgr A*, which, together with the previously reported steady X-ray emission, provides compelling evidence that the emission is coming from the accretion of gas onto a supermassive black hole at the Galactic Centre.     

基本电子结构（修订版）

本书提供了一个对解析的电子结构的说明，以便与计算的电子结构相互区分。这两者都是建立在单电子近似、局部密度理论和量子机理电子态确定的基础之上的。这两种方法都是以这些状态为基础。寻求合成的固体或者分子性质的预测。本书是1999年出版的教课书《固体的电子结构与性质》一书的修订版。书中增加了有关玻璃的一章，并且重新撰写了有关旋转轨道耦合、磁合金与锕系元素的章节。     

非线性嗅觉生理模型在一维序列识别中的应用

Water J Freeman教授在生理实验的基础上建立了一套基于嗅觉系统的神经网络模型。这套模型运用非线性动力学的方法较好的模拟了人体的嗅觉系统,并能够产生类似脑电的非周期背景信号。利用这个的基于嗅觉系统而建立的模型--K系列模型可以实现模式识别。本文首先从原理上介绍了K系列模型的拓扑结构和数学基础,然后通过实际的计算机数值模拟介绍了KII网络和KIII模型在实现一维序列识别时的方法和结果,简单说明了这个模型在模式识别上的特点。