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101.
Effectiveness of immune surveillance of intracellular viruses and bacteria depends upon a functioning antigen presentation
pathway that allows infected cells to reveal the presence of an intracellular pathogen. The antigen presentation pathway uses
virtually all endogenous polypeptides as a source to produce antigenic peptides that are eventually chaperoned to the cell
surface by MHC class I molecules. Intriguingly, MHC I molecules present peptides encoded not only in the primary open reading
frames but also those encoded in alternate reading frames. Here, we review recent studies on the generation of cryptic pMHC
I. We focus on the immunological significance of cryptic pMHC I, and the novel translational mechanisms that allow production
of these antigenic peptides from unconventional sources. 相似文献
102.
Lopez-Castejón G Baroja-Mazo A Pelegrín P 《Cellular and molecular life sciences : CMLS》2011,68(18):3095-3107
Plasticity is a well-known property of macrophages that is controlled by different changes in environmental signals. Macrophage
polarization is regarded as a spectrum of activation phenotypes adjusted from one activation extreme, the classic (M1), to
the other, the alternative (M2) activation. Here we show, in vitro and in vivo, that both M1 and M2 macrophage phenotypes
are tightly coupled to specific patterns of gene expression. Novel M2-associated markers were characterized and identified
as genes controlling the extracellular metabolism of ATP to generate pyrophosphates (PPi). Stimulation of M1 macrophages with
PPi dampens both NLR and TLR signaling and thus mediates cytokine production. In this context extracellular PPi enhanced the
resolution phase of a murine peritonitis model via a decrease in pro-inflammatory cytokine production. Therefore, our study
reveals an additional level of plasticity modulating the resolution of inflammation. 相似文献
103.
Important to the function of calpains is temporal and spatial regulation of their proteolytic activity. Here, we demonstrate
that cytoplasm-resident calpain 2 cleaves human nuclear topoisomerase I (hTOP1) via Ca2+-activated proteolysis and nucleoplasmic shuttling of proteases. This proteolysis of hTOP1 was induced by either ionomycin-caused
Ca2+ influx or addition of Ca2+ in cellular extracts. Ca2+ failed to induce hTOP1 proteolysis in calpain 2-knockdown cells. Moreover, calpain 2 cleaved hTOP1 in vitro. Furthermore,
calpain 2 entered the nucleus upon Ca2+ influx, and calpastatin interfered with this process. Calpain 2 cleavage sites were mapped at K158 and K183 of hTOP1. Calpain 2-truncated hTOP1 exhibited greater relaxation activity but remained able to interact with nucleolin and
to form cleavable complexes. Interestingly, calpain 2 appears to be involved in ionomycin-induced protection from camptothecin-induced
cytotoxicity. Thus, our data suggest that nucleocytoplasmic shuttling may serve as a novel type of regulation for calpain
2-mediated nuclear proteolysis. 相似文献
104.
The myogenic transcriptional network 总被引:2,自引:0,他引:2
105.
Good MF 《Cellular and molecular life sciences : CMLS》2011,68(7):1105-1113
Malaria presents a challenge to world health that to date has been beyond the abilities of researchers to conquer. This critique
presents some of the strategies employed by the parasite to overcome immunity and the immunological challenges that we face
to develop vaccines. A conclusion is that a vaccine must identify novel antigens or epitopes that are not normally immunogenic
and which are therefore not under immune pressure and most likely to be conserved between different strains. Such antigens
are most likely to be targets of cellular immunity. The case for a whole parasite blood stage vaccine is presented based on
these premises. 相似文献
106.
Marsango S Bonaccorsi di Patti MC Barra D Miele R 《Cellular and molecular life sciences : CMLS》2011,68(17):2919-2929
Prokineticins are proteins that regulate diverse biological processes including gastrointestinal motility, angiogenesis, circadian
rhythm, and innate immune response. Prokineticins bind two closed related G-protein coupled receptors (GPCRs), PKR1 and PKR2.
In general, these receptors act as molecular switches to relay activation to heterotrimeric G-proteins and a growing body
of evidence points to the fact that GPCRs exist as homo- or heterodimers. We show here by Western-blot analysis that PKR2
has a dimeric structure in neutrophils. By heterologous expression of PKR2 in Saccharomyces cerevisiae, we examined the mechanisms of intermolecular interaction of PKR2 dimerization. The potential involvement of three types
of mechanisms was investigated: coiled-coil, disulfide bridges, and hydrophobic interactions between transmembrane domains.
Characterization of differently deleted or site-directed PKR2 mutants suggests that dimerization proceeds through interactions
between transmembrane domains. We demonstrate that co-expressing binding-deficient and signaling-deficient forms of PKR2 can
re-establish receptor functionality, possibly through a domain-swapping mechanism. 相似文献
107.
108.
提出了一种用于时分多址突发通信系统中的IJF-OQPSK调制信号解调方法.介绍了电路的组成及原理,分析了系统的性能.该方法具有电路结构简单、数据恢复快、相位抖动小的优点,对提高突发通信系统的效率有显著作用.实验结果表明在时分多址突发通信系统中,该方法具有很好的使用价值. 相似文献
109.
An inflammatory drug prospect. 总被引:2,自引:0,他引:2
110.
Angela M. Ortega-León Maricela Villagrán-Santa Cruz J. Jamie Zú?iga-Vega Raúl Cueva-del Castillo Fausto R. Méndez-de la Cruz 《西北部美国博物学家》2011,69(1)
Asynchronous reproduction is a common phenomenon in high-elevation populations of lizards from Central México. Sperm storage in the reproductive tract of females is the mechanism for making oocyte fertilization possible. Our study addresses questions related to functional oviductal sperm storage of females mating on different dates throughout the reproductive season. A population of Sceloporus mucronatus with copulation in the summer and ovulation in the fall was chosen for this experiment. Eleven females that copulated in the field during June and 13 females that copulated in captivity during August were maintained in the laboratory until parturition. The number of pregnant females and the litter sizes produced in each experimental group were indicative of the viability of the stored sperm. Sperm stored in the reproductive tract of females were able to fertilize eggs after 4 months. No significant differences were found in the number of pregnant females between the 2 experimental groups nor in the litter sizes that they produced. We found that the amount of time sperm were held in the female reproductive tract (ca. 3 months) had no effect on the capacity of sperm to fertilize eggs. Histological examination of 8 oviducts collected before the mating season eliminated the possibility of sperm storage from one year to the next. In this system, sperm retention could have evolved as a response mechanism to deal with the asynchrony between sexes in the reproductive cycles. However, we cannot rule out alternative hypotheses. 相似文献