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排序方式: 共有197条查询结果,搜索用时 438 毫秒
71.
Otero G Biddau G Sánchez-Sánchez C Caillard R López MF Rogero C Palomares FJ Cabello N Basanta MA Ortega J Méndez J Echavarren AM Pérez R Gómez-Lor B Martín-Gago JA 《Nature》2008,454(7206):865-868
Graphite vaporization provides an uncontrolled yet efficient means of producing fullerene molecules. However, some fullerene derivatives or unusual fullerene species might only be accessible through rational and controlled synthesis methods. Recently, such an approach has been used to produce isolable amounts of the fullerene C(60) from commercially available starting materials. But the overall process required 11 steps to generate a suitable polycyclic aromatic precursor molecule, which was then dehydrogenated in the gas phase with a yield of only about one per cent. Here we report the formation of C(60) and the triazafullerene C(57)N(3) from aromatic precursors using a highly efficient surface-catalysed cyclodehydrogenation process. We find that after deposition onto a platinum (111) surface and heating to 750 K, the precursors are transformed into the corresponding fullerene and triazafullerene molecules with about 100 per cent yield. We expect that this approach will allow the production of a range of other fullerenes and heterofullerenes, once suitable precursors are available. Also, if the process is carried out in an atmosphere containing guest species, it might even allow the encapsulation of atoms or small molecules to form endohedral fullerenes. 相似文献
72.
73.
Ratje AH Loerke J Mikolajka A Brünner M Hildebrand PW Starosta AL Dönhöfer A Connell SR Fucini P Mielke T Whitford PC Onuchic JN Yu Y Sanbonmatsu KY Hartmann RK Penczek PA Wilson DN Spahn CM 《Nature》2010,468(7324):713-716
The elongation cycle of protein synthesis involves the delivery of aminoacyl-transfer RNAs to the aminoacyl-tRNA-binding site (A?site) of the ribosome, followed by peptide-bond formation and translocation of the tRNAs through the ribosome to reopen the A?site. The translocation reaction is catalysed by elongation factor G (EF-G) in a GTP-dependent manner. Despite the availability of structures of various EF-G-ribosome complexes, the precise mechanism by which tRNAs move through the ribosome still remains unclear. Here we use multiparticle cryoelectron microscopy analysis to resolve two previously unseen subpopulations within Thermus thermophilus EF-G-ribosome complexes at subnanometre resolution, one of them with a partly translocated tRNA. Comparison of these substates reveals that translocation of tRNA on the 30S subunit parallels the swivelling of the 30S head and is coupled to unratcheting of the 30S body. Because the tRNA maintains contact with the peptidyl-tRNA-binding site (P?site) on the 30S head and simultaneously establishes interaction with the exit site (E?site) on the 30S platform, a novel intra-subunit 'pe/E' hybrid state is formed. This state is stabilized by domain?IV of EF-G, which interacts with the swivelled 30S-head conformation. These findings provide direct structural and mechanistic insight into the 'missing link' in terms of tRNA intermediates involved in the universally conserved translocation process. 相似文献
74.
José Paulo L. Guadanucci Rafael Fonseca-Ferreira Renner Luiz Cerqueira Baptista Denis Rafael Pedroso 《Journal of Natural History》2016,50(39-40):2487-2497
Trechona diamantina sp. nov. is described from quartzite caves in Diamantina, central Minas Gerais State, Brazil. This represents the largest cavernicolous mygalomorph population ever recorded. The new species is the first Trechona recorded from a xeric habitat, namely the Rocky Fields on the Diamantina Plateau. T. diamantina sp. nov. is closely related to T. uniformis, both species possessing elongated male and female copulatory organs and both constructing distinct funnel-webs and sheet-webs in quartzite formations. T. diamantina sp. nov. is distinct in possessing less elongated embolus and receptacula seminis. A key for all valid species of the genus is given.
http://zoobank.org/urn:lsid:zoobank.org:pub:EB9E66B3-4701-470C-A80D-873E25F52042 相似文献
75.
Cristina García-Ruiz Domingo Lloris José Luis Rueda M. Carmen García-Martínez Luis Gil de Sola 《Journal of Natural History》2015,49(19-20):1191-1224
This study describes the four main demersal fish assemblages identified along the continental shelf and slope (30–800 m depth) of the northern Alboran Sea (western Mediterranean), based on the analysis of the MEDITS (International bottom trawl survey in the Mediterranean) 12-year data series. We collected 186 fish species belonging to three classes, 24 orders and 69 families. Taxonomically, the order Perciformes was the most diverse, represented by 18 families and 58 species. Each assemblage had particular characteristics of abundance, biomass, mean fish weight and species richness. The geographical differences associated with the distribution of some species occurred within shelf assemblages. The middle slope was characterised by the highest similarity between samples, probably due to its lower environmental variability compared to that of the other assemblages. 相似文献
76.
Regulation of flowering time by FVE, a retinoblastoma-associated protein 总被引:25,自引:0,他引:25
Ausín I Alonso-Blanco C Jarillo JA Ruiz-García L Martínez-Zapater JM 《Nature genetics》2004,36(2):162-166
77.
Isolation of an autotrophic ammonia-oxidizing marine archaeon 总被引:44,自引:0,他引:44
For years, microbiologists characterized the Archaea as obligate extremophiles that thrive in environments too harsh for other organisms. The limited physiological diversity among cultivated Archaea suggested that these organisms were metabolically constrained to a few environmental niches. For instance, all Crenarchaeota that are currently cultivated are sulphur-metabolizing thermophiles. However, landmark studies using cultivation-independent methods uncovered vast numbers of Crenarchaeota in cold oxic ocean waters. Subsequent molecular surveys demonstrated the ubiquity of these low-temperature Crenarchaeota in aquatic and terrestrial environments. The numerical dominance of marine Crenarchaeota--estimated at 10(28) cells in the world's oceans--suggests that they have a major role in global biogeochemical cycles. Indeed, isotopic analyses of marine crenarchaeal lipids suggest that these planktonic Archaea fix inorganic carbon. Here we report the isolation of a marine crenarchaeote that grows chemolithoautotrophically by aerobically oxidizing ammonia to nitrite--the first observation of nitrification in the Archaea. The autotrophic metabolism of this isolate, and its close phylogenetic relationship to environmental marine crenarchaeal sequences, suggests that nitrifying marine Crenarchaeota may be important to global carbon and nitrogen cycles. 相似文献
78.
Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease 总被引:112,自引:0,他引:112
Kim JH Auerbach JM Rodríguez-Gómez JA Velasco I Gavin D Lumelsky N Lee SH Nguyen J Sánchez-Pernaute R Bankiewicz K McKay R 《Nature》2002,418(6893):50-56
Parkinson's disease is a widespread condition caused by the loss of midbrain neurons that synthesize the neurotransmitter dopamine. Cells derived from the fetal midbrain can modify the course of the disease, but they are an inadequate source of dopamine-synthesizing neurons because their ability to generate these neurons is unstable. In contrast, embryonic stem (ES) cells proliferate extensively and can generate dopamine neurons. If ES cells are to become the basis for cell therapies, we must develop methods of enriching for the cell of interest and demonstrate that these cells show functions that will assist in treating the disease. Here we show that a highly enriched population of midbrain neural stem cells can be derived from mouse ES cells. The dopamine neurons generated by these stem cells show electrophysiological and behavioural properties expected of neurons from the midbrain. Our results encourage the use of ES cells in cell-replacement therapy for Parkinson's disease. 相似文献
79.
80.
J. L. R. Arrondo María José Sancho J. M. Macarulla 《Cellular and molecular life sciences : CMLS》1978,34(8):1099-1100
Summary Single injections of thyroid hormone (T3) produce liver glycogen depletion in chickens. This effect cannot be suppressed by protein synthesis inhibitors and is previous to the hormone-induced increase in protein synthesis. 相似文献