STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane

As the sole Ca2+ entry mechanism in a variety of non-excitable cells, store-operated calcium (SOC) influx is important in Ca2+ signalling and many other cellular processes. A calcium-release-activated calcium (CRAC) channel in T lymphocytes is the best-characterized SOC influx channel and is essential to the immune response, sustained activity of CRAC channels being required for gene expression and proliferation. The molecular identity and the gating mechanism of SOC and CRAC channels have remained elusive. Previously we identified Stim and the mammalian homologue STIM1 as essential components of CRAC channel activation in Drosophila S2 cells and human T lymphocytes. Here we show that the expression of EF-hand mutants of Stim or STIM1 activates CRAC channels constitutively without changing Ca2+ store content. By immunofluorescence, EM localization and surface biotinylation we show that STIM1 migrates from endoplasmic-reticulum-like sites to the plasma membrane upon depletion of the Ca2+ store. We propose that STIM1 functions as the missing link between Ca2+ store depletion and SOC influx, serving as a Ca2+ sensor that translocates upon store depletion to the plasma membrane to activate CRAC channels.     

Intrinsic dynamics of an enzyme underlies catalysis

A unique feature of chemical catalysis mediated by enzymes is that the catalytically reactive atoms are embedded within a folded protein. Although current understanding of enzyme function has been focused on the chemical reactions and static three-dimensional structures, the dynamic nature of proteins has been proposed to have a function in catalysis. The concept of conformational substates has been described; however, the challenge is to unravel the intimate linkage between protein flexibility and enzymatic function. Here we show that the intrinsic plasticity of the protein is a key characteristic of catalysis. The dynamics of the prolyl cis-trans isomerase cyclophilin A (CypA) in its substrate-free state and during catalysis were characterized with NMR relaxation experiments. The characteristic enzyme motions detected during catalysis are already present in the free enzyme with frequencies corresponding to the catalytic turnover rates. This correlation suggests that the protein motions necessary for catalysis are an intrinsic property of the enzyme and may even limit the overall turnover rate. Motion is localized not only to the active site but also to a wider dynamic network. Whereas coupled networks in proteins have been proposed previously, we experimentally measured the collective nature of motions with the use of mutant forms of CypA. We propose that the pre-existence of collective dynamics in enzymes before catalysis is a common feature of biocatalysts and that proteins have evolved under synergistic pressure between structure and dynamics.     

A plasma protein marker for population genetic studies of the desert tortoise ( Xerobates agassizi )

Fifty-seven individual plasma samples from desert tortoises ( Xerobates agassizi ) representing 10 separate populations were analyzed by polyacrylamide gel electrophoresis using alkaline buffers. An albumin-like protein was found to be polymorphic for two electromorphs in northern populations inhabiting the Mohave Desert Province while Sonoran Desert populations to the south were monomorphic. The genetic divergence demonstrated in this survey is similar to earlier studies and provides evidence for the Colorado River as a potential barrier to gene flow among tortoise populations. These data suggest that tortoise plasma, examined by various electrophoretic methods, may provide a nondestructive means of determining the broad regional origin of desert tortoises.     

Vegetation of the mima mounds of Kalsow Prairie, Iowa

Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 st1\:*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} Patterns of zonation along a saline meadow slope were studied. Different species associations were distributed in five zones along the slope which paralleled Utah Lake. The five zones, distinguished on the basis of dominant species and/or life form, were: saltgrass–annual weed, saltgrass-alkaligrass, saltgrass-forb, saltgrass, and spikerush. Soil, vegetation, and plant species data were taken. Patterns of change with respect to these factors were observed along the downslope gradient. Soil pH and soluble salts decreased downslope, while organic matter and moisture increased. Individual ions showed varying patterns. Vegetation and species patterns also varied with slope position. Annuals dominated the ridge tops, while sedge and rush cover were restricted to the slope base. Perennial forb distribution was shown to be correlated with elevated levels of micronutrients in the soil.     

Relationships of aspen ( Populus tremuloides ) to foraging patterns of beaver ( Castor canadensis ) in the Strawberry Valley of central Utah

Eight study sites were examined in Strawberry Valley, Utah, to assess the response of aspen ( Populus tremuloides Michx.) to cutting activities of beaver ( Castor Canadensis Warren & Hall) and to determine patterns by which the animals utilize aspen stands. Sites utilized by beaver, along with adjacent control plots in mature, uncut aspen stands, were sampled. Age-class profiles of control plots were composed of a broad age distribution with trees ranging from 3 to 108 years old. Age-class profiles for aspen sprouts in areas previously used by beavers were composed of trees averaging seven years of age with a range of 1 to 24 years. Age distribution of sprouts in areas used by beaver show a tendency to be skewed toward younger age classes. Average density of aspen in areas used by beaver was 15,800stems per hectare compared to 2,980 stems per hectare in controls. Stump densities in use areas ranged from 900 to 5,066 stems per hectare. Densities of stumps in the 0-5-cm size class were greater in areas used by beavers than in the corresponding size class in the mature forests. A regression equation describing age versus diameter relationships was calculated using data from 312 aspen trees. Total phenolics and mineral nutrients in the twigs and bark of mature aspen trees and aspen sprouts were also examined to determine if variations could explain foraging patterns of beaver in the valley. Total phenolics were highly variable between sampling groups, and differences were not significant. Twigs from mature aspen and aspen sprouts were significantly higher in nitrogen, phosphorus, and iron. Zinc was significantly higher in the bark of mature aspen trees and twigs of aspen sprouts. Calcium concentrations were significantly higher in mature aspen bark, and magnesium was significantly higher in mature aspen twigs.     

Spatial patterns of plant communities and differential weathering in Navajo National Monument, Arizona

Vegetation patterns in Navajo National Monument, Arizona, were studied over a five-year period from 1977 to 1981. Twelve distinct plant community types occur within the boundaries of the park. These communities are characterized and the dominant plant species of each are recorded. The relationships of parent material, soils, and moisture to plant communities are also discussed. It appears that discrete communities occupy soils of different characteristics, particularly with respect to amount of weathering of parent material.         

Habitat relationships of the blackbrush community ( Coleogyne ramosissima ) of southwestern Utah

Eight general study sites in the blackbrush ( Coleogyne ramosissima ) zone of southwestern Utah were examined. Soils data were gathered and plant cover was estimated. Shrubs were found to he positively correlated with shallow, sandy soil. Nonwoody plants were found to be positively correlated with deeper, silty soils. Cryptogamic soil crusts were positively correlated with silt and nitrogen in the soil and therefore may play a role in elevating soil fertility. Shrubs and grasses were negatively correlated. Management implications are discussed.         

Vegetation patterns in relation to slope position in the Castle Cliffs area of southern Utah

Vegetation patterns in relation to slope position were studied on four foothill knolls in the Castle Cliffs area of Washington County, Utah. Study plots were established at four different slope positions: ridge top, upper slope, lower slope, and floodplain. Exposed rock was highest on the ridge top; exposed soil was highest on the floodplain; soil depth increased downslope. Plant life form varied with respect to slope position. Grass, annuals, and cryptogamic crust cover was highest on the ridge top and shrubs were most prominent on the midslope. Forb cover gradually increased downslope. Succulents were restricted to the ridge top or floodplain. Species distribution was distinct and strongly correlated to slope position. Two sets of congeneric species showed strong patterns of niche separation. The vegetation of the slopes is highly distinct at the ridge top and floodplain and grades toward the midslope from both ends.         

Weather conditions in early summer and their effects on September blue grouse ( Dendragapus obscurus ) harvest

Relationships of temperature and precipitation to the reproductive success of blue grouse ( Dendragapus obscurus ) were investigated. Maximum and minimum temperatures followed similar patterns during the years 1976-1981 and showed no patterns relative to hatching success. Precipitation data, however, was variable. When significant amounts of precipitation fell during the last three weeks of the hatching period, chick survival and, therefore, recruitment were adversely affected. We suggest that precipitation occurring at the end of the hatch period reduces the September harvest of birds.     

Behavior and habitat preferences of ring-necked pheasants during late winter in central Utah

Ring-necked pheasant behavior and habitat preferences were studied during late February along benches of the Wasatch Mountains in central Utah. Seven behavioral categories were used to classify pheasant activities during three time periods of the day. Eating, alert, and movement behavior were the most frequent activities during all periods of the day. Significant differences (p < .01) were found between morning and midday behavior and between midday and evening behavior. Pheasants fed in open areas during morning hours then retreated into heavy cover during midday periods. The birds remained in heavy cover until late afternoon. Pheasants then moved away from heavy cover into semiopen areas to feed as evening approached.