Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours.

Mutations in the p53 tumour-suppressor gene are the most frequently observed genetic lesions in human cancers. To investigate the role of the p53 gene in mammalian development and tumorigenesis, a null mutation was introduced into the gene by homologous recombination in murine embryonic stem cells. Mice homozygous for the null allele appear normal but are prone to the spontaneous development of a variety of neoplasms by 6 months of age. These observations indicate that a normal p53 gene is dispensable for embryonic development, that its absence predisposes the animal to neoplastic disease, and that an oncogenic mutant form of p53 is not obligatory for the genesis of many types of tumours.     

行波型超声波电机定转子接触模型的研究

采用刚性定子和柔性转子的面接触模型,将摩擦层等效为分布的线性弹簧,建立了考虑摩擦材料剪切变形这种更符合实际情况的接触模型．探讨了电机参数对其性能的影响,通过与其他学者的研究成果相对照。验证了模型的有效性。     

真菌诱导物对丹参毛状根氧化酶活性的影响

从一种广谱病原真菌－大丽轮枝菌的菌丝体中制备激发子,用该激发子和商品生物制剂－酵母提取物分别诱导对数生长期的丹参毛状根,发现丹参毛状根过氧化物酶（POD）,过氧化氢酶（CAT）和多酚氧化酶（PPO）活性在诱导后均显著增加,三种酶的活性并随诱导时间和诱导剂的浓度的增加而改变,大丽轮枝菌激发子的作用强于酵母提取物,表现为诱导酶活性峰值较高和峰值时间提前。     

新型电致发光材料聚对苯乙炔的制备

报道了新型电致发光材料聚对苯乙炔的制备过程和有关测试结果。发现恒重后的双锍盐含有二个结晶水这一新现象，并确认最终产物为聚对苯乙炔。     

初始排污权拍卖的博弈分析

基于一级密封价格拍卖方式,建立了排污权交易制度中初始排污权分配的不完全信息静态博弈模型,对模型进行了分析,认为况标拍卖方式是初始排污权的一种合理的分配方式,并得到投标人越多,政府管理机构所得收益越高的结论。     

Direct splitting of water under visible light irradiation with an oxide semiconductor photocatalyst.

The photocatalytic splitting of water into hydrogen and oxygen using solar energy is a potentially clean and renewable source for hydrogen fuel. The first photocatalysts suitable for water splitting, or for activating hydrogen production from carbohydrate compounds made by plants from water and carbon dioxide, were developed several decades ago. But these catalysts operate with ultraviolet light, which accounts for only 4% of the incoming solar energy and thus renders the overall process impractical. For this reason, considerable efforts have been invested in developing photocatalysts capable of using the less energetic but more abundant visible light, which accounts for about 43% of the incoming solar energy. However, systems that are sufficiently stable and efficient for practical use have not yet been realized. Here we show that doping of indium-tantalum-oxide with nickel yields a series of photocatalysts, In(1-x)Ni(x)TaO(4) (x = 0-0.2), which induces direct splitting of water into stoichiometric amounts of oxygen and hydrogen under visible light irradiation with a quantum yield of about 0.66%. Our findings suggest that the use of solar energy for photocatalytic water splitting might provide a viable source for 'clean' hydrogen fuel, once the catalytic efficiency of the semiconductor system has been improved by increasing its surface area and suitable modifications of the surface sites.     

基于多源浮动车数据融合的道路交通运行评估

针对当前出租车浮动车数据覆盖范围和数据质量不足的问题,在现有数据特征分析的基础上引入百度手机导航和公交车浮动车导航数据,面向道路运行评估和拥堵管理提出了基于出行时间的交通运行指数算法和一种基于出租车、百度、公交车多源浮动车数据融合的道路交通运行评估方法,构建了多源数据融合规则和融合算法.以深圳为例进行了融合效果分析,结果表明所提出的多源数据融合方法效果显著,很大程度上提高了融合结果精度、数据覆盖率、数据可靠性和数据样本量,最后提出了主要应用方向和初步应用效果,能够为国内外城市开展交通运行评估工作提供借鉴.     

Symmetrical directional cloning: An efficient method to prepare hairpin RNA interference constructs

RNA interference (RNAi) is a loss-of-function approach by which double-stranded RNA (dsRNA) initiates degradation of homologous mRNAs in a sequence specific manner. The dsRNA molecules can be produced in vitro or in vivo, and can be introduced to cells in a number of ways. Here we report a more efficient method for the cloning of inverted repeat DNA fragments into expression vectors that can be transcribed into effective dsRNA molecules in vivo or in vitro. This method, named Symmetrical Directional Cloning (SDC), takes the advantage of compatible non-palindromic restriction enezyme sites, which allow one to directionally clone a single PCR product in both the sense and antisense orientations together into a vector. SDC allows for the directional cloning of inverted repeats using a single PCR product; it requires only one cut site on each side of the loop. Hence this method is more cost effective and less time-consuming. At least 21 commercially available restriction endonucleases can be used as cloning sites for the SDC method. The efficacy of dsRNA expression vectors prepared by SDC has been demonstrated by targeting a negative regulator of the signaling pathway mediating the response of cells to phytohormone, gibberellins (GA), in the aleurone cells.     

EB病毒及化学致癌物DNP对人胚鼻咽粘膜裸鼠移植的诱癌研究

用EBV,DNP及PMA分别或协同对人胚鼻咽粘膜裸鼠移植物进行诱癌研究,探讨上述因素在人实验性鼻咽癌发生中的作用。免疫荧光法检查证实,经B95-8上清液体外及体内转染的人胚鼻咽粘膜上皮表达EBNA。实验结果显示,实验各组均可见各种增生性改变,对照组仅见一般增生性病变。各诱癌组均可见癌前病变,组间无显著性差异。共出现癌8例(1)EBV组:原位癌1例。 (2)EBV+PMA组:早期浸润癌1例。(3)EBV+DNP组:原位癌及浸润癌各1例。(4)EBV+DNP+PMA组:浸润癌1例。 (5)DNP组:原位癌1例。(6)DNP+PMA组:原位癌及浸润癌各1例。结果提示EBV可能在人鼻咽上皮实验性癌变过程中起病因作用。本研究成功诱发了三例浸润癌,均系DAP与其它因素(PMA、EBV)协同作用,提示人鼻咽癌的发生可能与多种致癌因素的相互作用有关。     

管内流动换热过程的性能综合分析

基于管内流动换热方程,在最小初投资的条件下导出经济合理的管长、管径比与换热参数的关系式.同时对管内流动换热过程进行火用分析,得出火用损失率最小的最佳运行参数(Reopt),以及由换热准则关系式得出的最佳换热参数(Stopt),最后得到使管内流动换热过程的结构、流动与换热性能综合优化的管长管径比.