Chemotherapeutic effects of 4-isothiocyanato-4′-nitrodiphenylamine (C9333-Go/CGP 4540) on infections withNematospiroides dubius,Hymenolepis diminuta,Hymenolepis nana andSpirometra mansonoides

Summary 4-Isothiocyanato-4-nitrodiphenylamine possessed anthelmintic activity againstNippostrongylus dubius andHymenolepis nana in mice andH. diminuta in rats as determined by both egg counts and recovery of parasites at autopsy. No activity was detected against the cestode,Spirometra mansonoides, in cats.This investigation was supported in part by grants AI-10512 and AI-09483 from the National Institutes of Health. The authors are indebted to Dr P.P. Weinstein for providing animals infected withSpirometra mansonoides.     

Plasma growth hormone in the rabbit fetus. Relation to maturation of the liver and lung]

Day 25 after insemination is a date of peculiar importance in the maturation of several organs in the Rabbit fetus. From day 25 onward the fetal liver stores increasing amounts of glycogen and the lung stores increasing amounts of lecithins, concomitant with sudden rise in the activity of lung phosphatidic-acid phosphohydrolase. Earlier studies on decapitated fetuses established that glycogen storage in the liver is dependent on a dual hormonal control, comprising a pituitary hormone like growth hormone or prolactin (some placental hormones share the same activity) and corticosteroids (Jost, 1961). Since the variations in endogenous corticosteroids do not seem to herald these liver or lung changes (Mulay et al., 1973), a study was made of growth hormone. Plasma immunoreactive growth hormone--determined with a heterologous Rat system (Kervran et al., 1976)--increases eightfold between days 23 and 25. During the same time plasma prolactin does not change according to McNeily and Friesen, 1978, and to unpublished data obtained with Dr McNeilly. In preliminary assays, Rat growth hormone was seen to increase phosphorylase "a" activity in the lung of 18.5 day-old Rat fetuses, thus anticipating normal development. We suggest that growth hormone plays a role in initiating liver and lung maturation.     

Field bioassay of male Douglas-fir beetle compound 3-methylcyclohex-3-en-1-one

Summary Synthetic 3-methylcyclohex-3-en-1-one, or 3,3-MCH, decreased by 77% the flight aggregation ofDendroctonus pseudotsugae (Scolytidae) to sticky traps containing attractant (frontalin, -pinene, and resin). However, windthrown trees were protected by 3,3-MCH much less than is known to occur with the isomer 3,2-MCH.This research was supported in part by NSF grants BMS 72-02522 and DEB 77-11361 and the O.S.U. School of Forestry. We thank the administration of the Siuslaw National Forest for study sites, and Dr L.M. Libbey for GC/MS of 3,3-MCH. Oregon State University Ag. Exp. Sta. Tech. Paper No. 4313.     

Effects of D-α-aminoadipate on physiologically evoked responses of cat dorsal horn neurones

Summary Microelectrophoretic administration of D--aminoadipate reversibly reduced excitatory responses of cat dorsal horn neurones evoked by iontophoretic glutamate and non-noxious peripheral stimuli but did not influence similar responses evoked by noxious peripheral stimuli or iontophoretic substance P.     

Diurnal rhythm of active calcium transport in rat intestine

Summary The active calcium transport along the small intestine of adult rat was shown to exhibit daily rhythmic variations of considerable amplitude. Both the light-dark cycle and the time of food presentation acted as synchronizers of the rhythm observed.This work has been supported by Polish Academy of Sciences within the project MR II.1.1.6.     

Impaired amylase release from the parotid gland of rats treated with reserpine.

Using an automated system for the analysis of amylase, the release of this enzyme was compared in superfused parotid gland segments from control and reserpine treated rats. Stimulant-evoked amylase release was delayed and of smaller magnitude in the glands of the treated animals and a reduction of the transmembrane K+ gradient caused a smaller and short lasting reduction in Ach-evoked release of amylase in the glands from these animals.     

A prostaglandin-like activity in small intestine and postirradiation gastrointestinal syndrome

Summary A correlation between the postirradiation increase of the small intestine motility and the prostaglandin-like activity in this organ during gastrointestinal syndrome was observed. Indomethacin decreased the elevated motility of intestine and reduced the prostaglandin-like activity in this syndrome.Acknowledgments. The work was supported by the Polish Academy of Sciences.     

The effect of acidic polysaccharides and prostaglandin-like substances isolated from Propionibacterium acnes on granulocyte chemotaxis.

Three acidic polysaccharide (AP) fractions and the prostaglandin-like substances (PLS) isolated from P. acnes were investigated regarding their chemotactic activities on polymorphonuclear leukocytes. Both AP's and PLS induced a significant chemotatic response, which suggests their involvement in inflammatory acne vulgaris.     

Effect of insulin on immunological phagocytosis by macrophages.

It was shown that, in physiological concentrations, insulin enhances, in vitro, the immunological phagocytosis of sensitized sheep erythrocytes by cultured mouse peritoneal macrophages. Insulin seems to stimulate macrophage phagocytosis as a cholinomimetic agonist by increasing the intracellular levels of cyclic GMP.     

PPD-induced blastogenesis is auto-regulated by suppressor cells generated in vitro.

Suppressor cell induction can be demonstrated during antigen specific blastogenesis by using the same methods which have shown induction of suppressor cells by Con A. Since suppressor cells are rapidly generated during antigen specific blastogenesis, they must regulate the final level of blastogenesis induced during the seven day in vitro incubation.