全文获取类型
收费全文 | 33389篇 |
免费 | 63篇 |
国内免费 | 71篇 |
专业分类
系统科学 | 208篇 |
丛书文集 | 718篇 |
教育与普及 | 79篇 |
理论与方法论 | 252篇 |
现状及发展 | 14656篇 |
研究方法 | 1484篇 |
综合类 | 15695篇 |
自然研究 | 431篇 |
出版年
2013年 | 214篇 |
2012年 | 515篇 |
2011年 | 962篇 |
2010年 | 235篇 |
2008年 | 628篇 |
2007年 | 608篇 |
2006年 | 665篇 |
2005年 | 663篇 |
2004年 | 596篇 |
2003年 | 623篇 |
2002年 | 630篇 |
2001年 | 993篇 |
2000年 | 914篇 |
1999年 | 615篇 |
1992年 | 585篇 |
1991年 | 477篇 |
1990年 | 504篇 |
1989年 | 499篇 |
1988年 | 499篇 |
1987年 | 518篇 |
1986年 | 504篇 |
1985年 | 614篇 |
1984年 | 507篇 |
1983年 | 412篇 |
1982年 | 358篇 |
1981年 | 348篇 |
1980年 | 476篇 |
1979年 | 1013篇 |
1978年 | 896篇 |
1977年 | 895篇 |
1976年 | 596篇 |
1975年 | 655篇 |
1974年 | 978篇 |
1973年 | 835篇 |
1972年 | 859篇 |
1971年 | 1056篇 |
1970年 | 1390篇 |
1969年 | 1049篇 |
1968年 | 986篇 |
1967年 | 1049篇 |
1966年 | 872篇 |
1965年 | 651篇 |
1964年 | 162篇 |
1959年 | 364篇 |
1958年 | 529篇 |
1957年 | 449篇 |
1956年 | 366篇 |
1955年 | 322篇 |
1954年 | 370篇 |
1948年 | 199篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
971.
CACP, encoding a secreted proteoglycan, is mutated in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. 总被引:3,自引:0,他引:3
J Marcelino J D Carpten W M Suwairi O M Gutierrez S Schwartz C Robbins R Sood I Makalowska A Baxevanis B Johnstone R M Laxer L Zemel C A Kim J K Herd J Ihle C Williams M Johnson V Raman L G Alonso D Brunoni A Gerstein N Papadopoulos S A Bahabri J M Trent M L Warman 《Nature genetics》1999,23(3):319-322
Altered growth and function of synoviocytes, the intimal cells which line joint cavities and tendon sheaths, occur in a number of skeletal diseases. Hyperplasia of synoviocytes is found in both rheumatoid arthritis and osteoarthritis, despite differences in the underlying aetiologies of the two disorders. We have studied the autosomal recessive disorder camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP; MIM 208250) to identify biological pathways that lead to synoviocyte hyperplasia, the principal pathological feature of this syndrome. Using a positional-candidate approach, we identified mutations in a gene (CACP) encoding a secreted proteoglycan as the cause of CACP. The CACP protein, which has previously been identified as both 'megakaryocyte stimulating factor precursor' and 'superficial zone protein', contains domains that have homology to somatomedin B, heparin-binding proteins, mucins and haemopexins. In addition to expression in joint synovium and cartilage, CACP is expressed in non-skeletal tissues including liver and pericardium. The similarity of CACP sequence to that of other protein families and the expression of CACP in non-skeletal tissues suggest it may have diverse biological activities. 相似文献
972.
Laping NJ 《Cellular and molecular life sciences : CMLS》1999,56(5-6):371-377
Hepatocyte growth factor (HGF) is an injury-released growth factor with diverse effects on epithelial and endothelial cells. These effects include proliferation, migration, extracellular matrix production and tubulogenesis. These activities allow HGF to function as an organizer of repair processes that bring about restoration of tubular function following renal injury. However, while HGF has been demonstrated to accelerate recovery of renal function after an acute insult, prolonged exposure to elevated levels of HGF can reduce renal function and may contribute to progressive renal disease. This review will describe the cellular activities of HGF, how they pertain to renal repair and the therapeutic application of regulating HGF activity in acute versus chronic renal disease. 相似文献
973.
A humanized model for multiple sclerosis using HLA-DR2 and a human T-cell receptor 总被引:14,自引:0,他引:14
Madsen LS Andersson EC Jansson L krogsgaard M Andersen CB Engberg J Strominger JL Svejgaard A Hjorth JP Holmdahl R Wucherpfennig KW Fugger L 《Nature genetics》1999,23(3):343-347
Multiple sclerosis (MS) is a complex chronic neurologic disease with a suspected autoimmune pathogenesis. Although there is evidence that the development of MS is determined by both environmental influences and genes, these factors are largely undefined, except for major histocompatibility (MHC) genes. Linkage analyses and association studies have shown that susceptibility to MS is associated with genes in the human histocompatibility leukocyte antigens (HLA) class II region, but the contribution of these genes to MS disease development less compared with their contribution to disorders such as insulin-dependent diabetes mellitus. Due to the strong linkage disequilibrium in the MHC class II region, it has not been possible to determine which gene(s) is responsible for the genetic predisposition. In transgenic mice, we have expressed three human components involved in T-cell recognition of an MS-relevant autoantigen presented by the HLA-DR2 molecule: DRA*0101/DRB1*1501 (HLA-DR2), an MHC class II candidate MS susceptibility genes found in individuals of European descent; a T-cell receptor (TCR) from an MS-patient-derived T-cell clone specific for the HLA-DR2 bound immunodominant myelin basic protein (MBP) 4102 peptide; and the human CD4 coreceptor. The amino acid sequence of the MBP 84-102 peptide is the same in both human and mouse MBP. Following administration of the MBP peptide, together with adjuvant and pertussis toxin, transgenic mice developed focal CNS inflammation and demyelination that led to clinical manifestations and disease courses resembling those seen in MS. Spontaneous disease was observed in 4% of mice. When DR2 and TCR double-transgenic mice were backcrossed twice to Rag2 (for recombination-activating gene 2)-deficient mice, the incidence of spontaneous disease increased, demonstrating that T cells specific for the HLA-DR2 bound MBP peptide are sufficient and necessary for development of disease. Our study provides evidence that HLA-DR2 can mediate both induced and spontaneous disease resembling MS by presenting an MBP self-peptide to T cells. 相似文献
974.
Hazan J Fonknechten N Mavel D Paternotte C Samson D Artiguenave F Davoine CS Cruaud C Dürr A Wincker P Brottier P Cattolico L Barbe V Burgunder JM Prud'homme JF Brice A Fontaine B Heilig B Weissenbach J 《Nature genetics》1999,23(3):296-303
Autosomal dominant hereditary spastic paraplegia (AD-HSP) is a genetically heterogeneous neurodegenerative disorder characterized by progressive spasticity of the lower limbs. Among the four loci causing AD-HSP identified so far, the SPG4 locus at chromosome 2p2-1p22 has been shown to account for 40-50% of all AD-HSP families. Using a positional cloning strategy based on obtaining sequence of the entire SPG4 interval, we identified a candidate gene encoding a new member of the AAA protein family, which we named spastin. Sequence analysis of this gene in seven SPG4-linked pedigrees revealed several DNA modifications, including missense, nonsense and splice-site mutations. Both SPG4 and its mouse orthologue were shown to be expressed early and ubiquitously in fetal and adult tissues. The sequence homologies and putative subcellular localization of spastin suggest that this ATPase is involved in the assembly or function of nuclear protein complexes. 相似文献
975.
Many hormones and neurotransmitters evoke Ca2+ release from intracellular stores, often triggering agonist-specific signatures of intracellular Ca2+ concentration. Inositol trisphosphate (InsP3) and cyclic adenosine 5'-diphosphate-ribose (cADPR) are established Ca2+-mobilizing messengers that activate Ca2+ release through intracellular InsP3 and ryanodine receptors, respectively. However, in pancreatic acinar cells, neither messenger can explain the complex pattern of Ca2+ signals triggered by the secretory hormone cholecystokinin (CCK). We show here that the Ca2+-mobilizing molecule nicotinic acid adenine dinucleotide phosphate (NAADP), an endogenous metabolite of beta-NADP, triggers a Ca2+ response that varies from short-lasting Ca2+ spikes to a complex mixture of short-lasting (1-2s) and long-lasting (0.2-1 min) Ca2+ spikes. Cells were significantly more sensitive to NAADP than to either cADPR or InsP3, whereas higher concentrations of NAADP selectively inactivated CCK-evoked Ca2+ signals in pancreatic acinar cells, indicating that NAADP may function as an intracellular messenger in mammalian cells. 相似文献
976.
Cytotoxic T-cell immunity to virus-infected non-haematopoietic cells requires presentation of exogenous antigen 总被引:16,自引:0,他引:16
Cytotoxic T lymphocytes (CTLs) are thought to detect viral infections by monitoring the surface of all cells for the presence of viral peptides bound to major histocompatibility complex (MHC) class I molecules. In most cells, peptides presented by MHC class I molecules are derived exclusively from proteins synthesized by the antigen-bearing cells. Macrophages and dendritic cells also have an alternative MHC class I pathway that can present peptides derived from extracellular antigens; however, the physiological role of this process is unclear. Here we show that virally infected non-haematopoietic cells are unable to stimulate primary CTL-mediated immunity directly. Instead, bone-marrow-derived cells are required as antigen-presenting cells (APCs) to initiate anti-viral CTL responses. In these APCs, the alternative (exogenous) MHC class I pathway is the obligatory mechanism for the initiation of CTL responses to viruses that infect only non-haematopoietic cells. 相似文献
977.
A spelling device for the paralysed 总被引:29,自引:0,他引:29
Birbaumer N Ghanayim N Hinterberger T Iversen I Kotchoubey B Kübler A Perelmouter J Taub E Flor H 《Nature》1999,398(6725):297-298
978.
Molecular basis of triclosan activity 总被引:19,自引:0,他引:19
Levy CW Roujeinikova A Sedelnikova S Baker PJ Stuitje AR Slabas AR Rice DW Rafferty JB 《Nature》1999,398(6726):383-384
979.
A capsaicin-receptor homologue with a high threshold for noxious heat 总被引:60,自引:0,他引:60
980.
Trans-gender induction of hair follicles 总被引:24,自引:0,他引:24