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991.
李莹 《辽宁师专学报(自然科学版)》2014,(2):20-22
通过对辽阳职业技术学院生物制药专业2010级、2011级学生顶岗实习情况的调查分析,构建"五化三落实"顶岗实习管理机制,提出顶岗实习是培养高技能人才的重要环节,是充分就业的有效途径. 相似文献
992.
993.
Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line. 总被引:114,自引:0,他引:114
A significant aspect of primary hepatic carcinoma in man is the high positive correlation of hepatocellular carcinoma with infection with hepatitis B virus (HBV)1. Analysis of the relationship between HBV infection and oncogenesis is difficult because natural infection with HBV is limited to man and experimental infection has been achieved only in chimpanzees and gibbons. Furthermore, because HBV has not been successfully propagated in cell culture, basic study of virus-cell interaction of the aetiological agent of one of the most widespread infections of man has been impossible. Recently, however, a cell line (PLC/PRF/5) derived from a human hepatoma biopsy was described which produces the HRV surface antigen (HBsAg) and so provides a tool for the experimental investigation of HBV in viro. We now report the derivation and characterisation of two additional cell lines primary liver carcinomas. In contrast to the PLC/PRF/5 cell line, these cell lines retain the capacity to synthesise many human plasma proteins, including both albumin and alpha-fetoprotein (AFP). One of these lines also produces BHsAg. We also present evidence that HBsAg synthesis and secretion in this cell line are correlated with the growth state of the culture. This finding is in contrast to the continuous HBsAg production found in the PLC/PRF/5 cell line. 相似文献
994.
High resolution studies on the crystal structure of glycogen phosphorylase b have identified the catalytic site to which the substrate glucose-1-phosphate binds strongly with some local conformational changes. The site is situated 8 A (phosphate-to-phosphate distance) from pyridoxal phosphate, an essential cofactor of all glycogen phosphorylases. The catalytic site is 33 A from the site in the N-terminal portion of the molecule to which adenine nucleotides bind. In contrast to phosphorylase a (the active form of the enzyme which is phosphorylated at Ser 14), the positions of the first 19 residues of phosphorylase b are not well defined. 相似文献
995.
996.
杨月红 《西安联合大学学报》1999,(2)
综述了近年来国内外在昆虫精子获能与顶体反应方面的研究概况,并系统地简述了昆虫精子顶体复合体基本结构,以及目前用于动物精子获能与顶体反应方面先进的方法和技术,同时对昆虫受精机理的研究进行了展望 相似文献
997.
中祁连地块东段元古宙基底湟源群沉积构造环境 总被引:6,自引:0,他引:6
通过对中祁连地块东段元古宙基底湟源群原岩建造及其地球化学特征的研究,认为:湟源群主要由各种千枚岩、片岩、变粒岩、大理岩、石英岩和斜长角闪岩等组成的中浅变质的表壳岩组合;其原岩为一套由陆源碎屑岩、不纯碳酸盐岩、中基性大山岩等组成的火山岩沉积岩组合;湟源群中变质沉积岩系中沉积韵律、沉积条带构造的广泛发育以及浊积层的存在等,指示了其原岩建造具有复理石或类复理石建造的特征;其中变质杂砂岩的地球化学特征类似于大陆岛弧或安底斯岛弧型杂砂岩的特征;湟源群变质中基性火山岩的地球化学特征类似于岛弧或活动大陆边缘的钙碱性火山岩的特征。经综合分析得出,湟源群沉积构造环境可能为陆缘弧后盆地且靠近大陆一侧。 相似文献
998.
选择水、乙醇作为助剂,利用六甲基二硅氮烷(HMDS)在气固流化床中对纳米SiO2进行表面改性处理,采用FT—IR、TEM等多种手段表征了HMDS对纳米SiO2的改性效果。水和乙醇的引入能有效提高HMDS对纳米SiO2的改性效果及纳米SiO2的疏水程度,改善其在有机相中的分散性。极性小分子助剂在HMDS改性纳米SiO2中的作用机理分析表明:水可以促进HMDS水解。又可以补充纳米SiO2的表面羟基;乙醇一方面与纳米SiO2发生酯化反应,另一部分作为分散HMDS的溶剂。 相似文献
999.
Menstrual cycle alters face preference. 总被引:23,自引:0,他引:23
I S Penton-Voak D I Perrett D L Castles T Kobayashi D M Burt L K Murray R Minamisawa 《Nature》1999,399(6738):741-742
1000.
Immunization with amyloid-beta attenuates Alzheimer-disease-like pathology in the PDAPP mouse. 总被引:121,自引:0,他引:121
D Schenk R Barbour W Dunn G Gordon H Grajeda T Guido K Hu J Huang K Johnson-Wood K Khan D Kholodenko M Lee Z Liao I Lieberburg R Motter L Mutter F Soriano G Shopp N Vasquez C Vandevert S Walker M Wogulis T Yednock D Games P Seubert 《Nature》1999,400(6740):173-177
Amyloid-beta peptide (Abeta) seems to have a central role in the neuropathology of Alzheimer's disease (AD). Familial forms of the disease have been linked to mutations in the amyloid precursor protein (APP) and the presenilin genes. Disease-linked mutations in these genes result in increased production of the 42-amino-acid form of the peptide (Abeta42), which is the predominant form found in the amyloid plaques of Alzheimer's disease. The PDAPP transgenic mouse, which overexpresses mutant human APP (in which the amino acid at position 717 is phenylalanine instead of the normal valine), progressively develops many of the neuropathological hallmarks of Alzheimer's disease in an age- and brain-region-dependent manner. In the present study, transgenic animals were immunized with Abeta42, either before the onset of AD-type neuropathologies (at 6 weeks of age) or at an older age (11 months), when amyloid-beta deposition and several of the subsequent neuropathological changes were well established. We report that immunization of the young animals essentially prevented the development of beta-amyloid-plaque formation, neuritic dystrophy and astrogliosis. Treatment of the older animals also markedly reduced the extent and progression of these AD-like neuropathologies. Our results raise the possibility that immunization with amyloid-beta may be effective in preventing and treating Alzheimer's disease. 相似文献