A new chromatographic method for fast separation of active proteins

A new method with only a single chromatographic column to accomplish the separation of intact protein molecules by the combination of ion exchange chromatography and hydrophobic interaction chromatography is firstly presented. By selecting twice a suitable stationary phase, mobile phase, buffer exchange condition, and sample injection, some proteins usually required to separate with two-dimensional chromatography can now be accomplished only using the single column in one hour. In addition, the separated proteins can maintain their original three-or four-dimensional molecular structure. It would be expected that this method can also provide a new thought for sample pre-fractionation in proteomic investigation, especially, for the limitation of sample source.     

U-Pb dating of detrital zircons from the Wudangshan Group in the South Qinling and its geological significance

U-Pb dating was made by the LA-ICP-MS method for detrital zircons from the Wudangshan volcanic-sedimentary succession in the South Qinling. Samples comprise quartz sandstones of the Wudangshan Group collected from the base of the Yangping Formation and an upper layer of the Shuangtai Formation overlain its volcanic sequence, and two river-sand collections from the drainage systems cutting across the two formations, respectively. The results show that the Yangping detrital zircons are dominated by 830–780 Ma grains with a minor population of ~1.0–0.84 Ga and sporadic grains of ~2.6, ~2.4 and 2.0 Ga, whereas the Shuangtai zircons yield an upper intercept age of 763±33 Ma, identical to the timing of the Wudangshan volcanism within error, with few concordant grains of 1.9 and 0.86 Ga. Age spectra for the two river-sand samples are similar to those of the Yangping and Shuangtai Formations, respectively. It thus suggests that the Wudangshan strata are less than 780 Ma, whereas their major detrital zircon populations of 1.0–0.85 Ga and 830–780 Ma are consistent with timing of the Hannan magmatic activities along the northwestern margin of the South China Block. This suggests a Hannan or adjacent area provenance for the Wudangshan strata. The Wudang area is characterized by rift-related igneous events at ~755 and ~680 Ma, respectively, pointing to a tectono- magmatic history different from the Hannan area. It is inferred that the ~755 Ma magmatism is likely to indicate a separation of the South China Block from the supercontinent Rodinia, while the ~680 Ma event suggests a further split between the South Qinling and some unknown continent.     

Interaction of aloe-emodin with human serum albumin

The presence of several high affinity binding sites on human serum albumin (HSA) makes it a possible target for many drugs. This study is designed to examine the effect of aloe-emodin on HSA by fluo-rescence, CD spectroscopy and molecular modeling. The results of fluorescence measurements sug-gested that the hydrophobic interaction was the predominant intermolecular force stabilizing the AE-HSA complex, which was in good agreement with the result of molecular modeling study. And the enthalpy change ΔH0 and the entropy change ΔS0 were calculated to be -7.041 kJ·mol-1 and 76.619 J·mol-1·K-1 according to the Van't Hoff equation. The alterations of protein secondary structure in the presence of AE in aqueous solution were quantitatively calculated from CD spectra, and the content of α-helices obviously increased.     

Applications of membrane distillation technology in energy transformation process-basis and prospect

Membrane distillation technology is a new type of efficient separation technology that combines traditional distillation technology and membrane separation technology. In the study, applications of membrane distillation technology in thermal engineering and refrigerating engineering with typical energy transformation process were presented. Desorption and regeneration process of saline solution by vacuum membrane distillation was proposed on the basis of the concentration and separation properties of membrane distillation. Membrane distillation technology could be used in lithium bromide absorption refrigeration system, energy storage system, and the regeneration process of liquid desiccant solution in temperature-humidity independent control air-conditioning system. The aim of the applications was to use the low-grade energy such as waste heat, solar energy and geothermal energy adequately and to improve the available temperature difference of heat source. According to latent heat transfer and thermal conduction across the membrane in direct contact membrane distillation process, a novel membrane heat exchanger with both heat transfer and mass transfer processes was proposed. The heat exchanger could be used as the solution heat exchanger of lithium bromide absorption refrigeration system and as the special heat exchanger that recovered heat and pure water simultaneously. Some feasible process flows about the applications of membrane distillation technology to energy transformation process were listed and analyzed. Finally, future research emphases were indicated.     

Valence electron structure analysis of refining mechanism of Sc and Ti additions on aluminum

The mechanism of the difference of refining effect between Sc and Ti adding to aluminum can not be explained substantially with traditional theory. Valence electron structures of Al-Ti and Al-Sc alloys have been studied by using the empirical electron theory of solids and molecules (EET). The covalent bond electron numbers and interfacial electron density differences are calculated. The conclusion is that, in the two alloys, different covalent bond electron numbers of nucleation particles, and different electron densities on the interface between the second phase particles and the matrix, fundamentally lead to the difference of refining effect between Sc and Ti adding to aluminum. Supported by the Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20050003042)     

Inhibiting effect of melittin on pathogens of crops

The spectrum of antimicrobial effects of melittin were investigated on 19 pathogens by using a cylin-der-plate method with serial dilutions. Bacteriostatic efficiency and possible mechanistic effects were monitored via growth curves. The mechanism of inhibition was further analyzed by SDS-PAGE, flow cytometry and electron microscopy. Melittin had a wide inhibition spectrum and killed pathogens ef-fectively, and bacteriostatic action was influenced by factors such as pH and temperature. We eluci-dated three inhibitory mechanisms: melittin integrated with the cell membrane causing cell bursting and cytoplasm release, inhibited the synthesis of proteins and caused the cytoplasm to condense, and delayed pathogens in phase I (or phase G1) so that they could not complete the cell cycle. These re-sults suggest that melittin could serve as a broad-spectrum biological pesticide with fast-action and high-efficiency.     

Batching Scheduling in a Two-Level Supply Chain with Earliness and Tardiness Penalties

This paper studies the batch sizing scheduling problem with earliness and tardiness penalties which is closely related to a two-level supply chain problem. In the problem, there are K customer orders, where each customer order consisting of some unit length jobs has a due date. The jobs are processed in a common machine and then delivered to their customers in batches, where the size of each batch has upper and lower bounds and each batch may incur a fixed setup cost which can also be considered a fixed delivery cost. The goal is to find a schedule which minimizes the sum of the earliness and tardiness costs and the setup costs incurred by creating a new batch. The authors first present some structural properties of the optimal schedules for single-order problem with an additional assumption (a): The jobs are consecutively processed from time zero. Based on these properties, the authors give a polynomial-time algorithm for single-order problem with Assumption (a). Then the authors give dynamic programming algorithms for some special cases of multiple-order problem with Assumption (a). At last, the authors present some structural properties of the optimal schedules for single-order problem without Assumption (a) and give a polynomial-time algorithm for it.     

Orthogonal Arrays Robust to a Specified Set of Nonnegligible Effects

This paper considers experimental situations where the interested effects have to be orthogonal to a set of nonnegligible effects.It is shown that various types of orthogonal arrays with mixed strength are A-optimal for estimating the parameters in ANOVA high dimension model representation.Both cases including interactions or not are considered in the model.In particularly,the estimations of all main effects are A-optimal in a mixed strength(2,2)_3 orthogonal array and the estimations of all main effects and two-factor interactions in G_1×G_2 are A-optimal in a mixed strength(2,2)_4 orthogonal array.The properties are also illustrated through a simulation study.     

Approximating the Spectral Abscissa for Switched Linear Systems via Coordinate Transformations

In this paper,an approach of square coordinate transformation is proposed to approximate the spectral abscissa for continuous-time switched linear systems.By applying elementary transformations iteratively,a series of minimums of least μ1 matrix set measures are obtained,which are utilized to approximate the spectral abscissa of the switched system.The approach is developed into tractable numerical algorithms that provide upper bound estimates of the spectral abscissa.Numerical simulations show the effectiveness of the proposed method.     

Empirical likelihood for partially linear models under negatively associated errors

This paper proposes to use the blockwise empirical likelihood(EL) method to construct the confidence regions for the regression vector β in a partially linear model under negatively associated errors. It is shown that the blockwise EL ratio statistic for β is asymptotically χ~2 distributed. The result is used to obtain an EL-based confidence region for β. Results of a simulation study on the finite sample performance of the proposed confidence regions are reported.