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101.
Characterization and relative abundance of maxi-chloride channels in Epstein-Barr virus (EBV) producer: B95-8 cells 总被引:2,自引:0,他引:2
T. H. Yeh M. C. Tsai S. Y. Lee M. M. Hsu 《Cellular and molecular life sciences : CMLS》1996,52(8):818-826
Several Epstein-Barr virus (EBV)-transformed cell lines were used to investigate the pathogenesis of lymphoproliferative diseases and nasopharyngeal carcinoma. The studies focus on the events occurring inside the membrane. On only one occasion, the cell membrane of EBV-transformed B lymphocytes from a cystic fibrosis patient was found to express defective Cl channels (CFTR; Cystic Fibrosis Transmembrane conductance Regulator), as in the airway epithelial cell. No other type of channel in EBV-transformed cells has so far been investigated. In this study, the cell membrane of the B95-8 cell was examined by the patch-clamp technique and compared to the non-EBV-infected BJAB cell. The high conductance (300 pS) maxi-chloride (Cl) channel activity was the most frequently observed event in inside-out configurations. Under similar experimental conditions, we have found a significantly higher probability of detecting maxi-Cl channel activity on the cell membrane of B95-8 cells (69%) than on BJAB cells (27%), or as previously reported on resting murine B lymphocytes (38%) or intact human T lymphocytes (37%). The relative abundance of the maxi-Cl channel on B95-8 cells may be linked to EBV infection and/or secretory ability. 相似文献
102.
ZHOU HuaiChun HUANG ZhiFeng CHENG Qiang LU We QIU Kui CHEN Chen HSU Pei-feng 《科学通报(英文版)》2011,56(10):962-968
The inferior mirage from road surfaces is a common phenomenon, which can be easily seen in everyday life. It has been recognized
in the literature as a light refraction phenomenon due to the refractive index gradient caused by the temperature gradient
in the air strata above the road surfaces. However, it was also suggested that the mirage is just a phenomenon of specular
reflection at grazing incidence. Because of the lack of reasonable and quantitative evidence, the generally accepted light
refraction theory has not yet been refuted. Here we show some mirror-like reflection images captured from a road surface stretch
in Yujiashan North Road, Wuhan, China, when there was no obvious temperature gradient on or above the road, measured on a
winter day in December 2009. This provided direct evidence to doubt the temperature induced light refraction mechanism of
the inferior mirage. Furthermore, the critical grazing angle of about 0.2° to the road plane where the mirror-like reflection
appears could not make the rough surface scatter incident light as a smooth surface according to the Rayleigh criterion. Therefore
the phenomenon is a mirrorlike observation effect of scattering from the surface, which cannot be entirely explained by light
refraction via air strata. The results demonstrate that the image-formation mechanism and the observer-based-analysis method
shown here potentially offer a means of understanding a wide range of scattering phenomena from rough surfaces at grazing
angle; for example, the superior mirages of unusual brightness occasionally observed over frozen lakes and the off-specular
reflection phenomenon. 相似文献