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981.
XIA Chun XU Guangxian LIN Changyou HU Tuanjun YAN Ruoqian George F GAO 《自然科学进展(英文版)》2004,14(5):403-410
In order to clarify the molecular sequences,allelic polymorphism and the tertiary structure of grass carp (Ctenophayngodon idellus) MHC class I,and to further study their relationship with disease resistances,grass carp MHC class I gene (Ctid-MHC I) was cloned from a cDNA library and the allelic polymorphism in the population was investigated.The results showed that most of the variations exist in the peptide-binding domain (PBD) and high polymorphism was identified in the Ctid-MHC I allelic genes from 12 individuals.Based on the genetic distance,Ctid-MHC class I can be classified into 6 types (from Ctid-MHC I-UA to Ctid-MHC I-UF) which were subdivided into 9 lineages (from A to I).Comparison of the Ctid-MHC I among animals and humans showed that the key amino acids of the peptide binding sites are conserved.Analysis of the tertiary structure of the PBD between Grass carp and human crystallographic data of HLA-A2,the variation with insertion or deletion was found in eight regions (A~H).The phylogenetic tree of MHC class I indicates the evolution of MHC class I among grass carp,fish,amphibian,birds,higher vertebrates and humans. 相似文献
982.
983.
Clutton-Brock TH Hodge SJ Spong G Russell AF Jordan NR Bennett NC Sharpe LL Manser MB 《Nature》2006,444(7122):1065-1068
In most animals, the sex that invests least in its offspring competes more intensely for access to the opposite sex and shows greater development of secondary sexual characters than the sex that invests most. However, in some mammals where females are the primary care-givers, females compete more frequently or intensely with each other than males. A possible explanation is that, in these species, the resources necessary for successful female reproduction are heavily concentrated and intrasexual competition for breeding opportunities is more intense among females than among males. Intrasexual competition between females is likely to be particularly intense in cooperative breeders where a single female monopolizes reproduction in each group. Here, we use data from a twelve-year study of wild meerkats (Suricata suricatta), where females show high levels of reproductive skew, to show that females gain greater benefits from acquiring dominant status than males and traits that increase competitive ability exert a stronger influence on their breeding success. Females that acquire dominant status also develop a suite of morphological, physiological and behavioural characteristics that help them to control other group members. Our results show that sex differences in parental investment are not the only mechanism capable of generating sex differences in reproductive competition and emphasize the extent to which competition for breeding opportunities between females can affect the evolution of sex differences and the operation of sexual selection. 相似文献
984.
Studies of intracellular traffic in yeast and mammalian systems have implicated members of the Rab family of small GTP-binding proteins as regulators of membrane fusion. We have used the patch clamp technique to measure exocytotic fusion events directly and investigate the role of GTP-binding proteins in regulating exocytosis in mast cells. Intracellular perfusion of mast cells with GTP-gamma S is sufficient to trigger complete exocytotic degranulation in the absence of other intracellular messengers. Here we show that GTP is a potent inhibitor of GTP-gamma S-induced degranulation, indicating that sustained activation of a GTP-binding protein is sufficient for membrane fusion. We have found that synthetic oligopeptides, corresponding to part of the effector domain of Rab3a, stimulate complete exocytotic degranulation, similar to that induced by GTP-gamma S. The response is selective for Rab3a sequence and is strictly dependent on Mg2+ and ATP. This suggests that sustained activation of a Rab3 protein causes exocytotic fusion. The peptide response can be accelerated by GDP-beta S, suggesting that Rab3a peptides compete with endogenous Rab3 proteins for a binding site on a target effector protein, which causes fusion on activation. 相似文献
985.
研究了青石棉、温石棉、直闪石和铁石棉与不同浓度N,N-二甲基苯胺(DMA)在110℃的吸附氧化反应,测定了产物的紫外可见光谱、红外光谱、元素分析和热分析数据.结果表明,四种石棉均能使DMA氧化为甲基紫.质子可以提高石棉的氧化能力.此外,反应过程还生成一种紫外可见光最大吸收为340nm的物质,其结构有待进一步鉴定. 相似文献
986.
Holocene lake deposits of Bosten Lake, southern Xinjiang, China 总被引:9,自引:0,他引:9
B.Wünnemann 《科学通报(英文版)》2003,48(14):1429-1432
A 9.25-m-long sediment core from Bosten Lake,Xinjiang, provides detailed information about changes in the water budget and biological acticity over the last 8400 cal-endar years. The chronology is constructed from six AMS radiocarbon dates on the terrestrial plant remains. Based onanalyses of TOC, CO3, detrital compounds and biogenic SiO2,lake level fluctuations and periods of remarkably-negative water budget appeared at 8.4-8.2 cal ka, 7.38-7.25 cal ka,5.7-5.5 cal ka, 3.7-3.4 cal ka and 3.3-2.9 cal ka, respec-tively. As they are in-phase with low lake levels at Sumxl Co and Bangong Co in western Tibet Plateau and with paleo-lakes in Inner Mongolia, a climate-induced change to some-what drier and warmer conditions is inferred. A further drop in lake level after 1320 AD of about 200 yr duration may heattributed to a negative water balance prior to the main phase of the Little Ice Age. Deep and stable lake phases of 1500 yr and 1800 yr duration at 7.2-5.7 cal ka and 5.5-3.7cal ka coincide with maximum moisture during the Holocene Megathermai in China. The long term trend towards aridity since about 4.3 cal ka can dearly be recognised. The reduced water budget of Bosten Lake from 640-1200 AD may be attributed to local effects. 相似文献
987.
A voltage-gated ion channel model inferred from the crystal structure of alamethicin at 1.5-A resolution 总被引:31,自引:0,他引:31
The crystal structure of alamethicin in nonaqueous solvent has been determined, and refined at 1.5-A resolution. The molecular conformation of the three crystallographically independent molecules is largely alpha-helical with a bend in the helix axis at an internal proline residue. The helix structure is highly amphipathic as most of the solvent-accessible polar atoms lie on a narrow strip of surface parallel to the helix axis. Molecular models for the voltage-gated ion channel, with n-fold symmetry and based on the molecular conformations observed in the crystal, are characterized by strong surface complementarity, a hydrophilic interior and a hydrophobic exterior. The channel structures are stabilized by a hydrated annulus of hydrogen-bonded glutamine residues which produce the greatest restriction in the channel diameter. 相似文献
988.
989.
990.
Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses 总被引:8,自引:0,他引:8
RJ O'Connell MR Thon S Hacquard SG Amyotte J Kleemann MF Torres U Damm EA Buiate L Epstein N Alkan J Altmüller L Alvarado-Balderrama CA Bauser C Becker BW Birren Z Chen J Choi JA Crouch JP Duvick MA Farman P Gan D Heiman B Henrissat RJ Howard M Kabbage C Koch B Kracher Y Kubo AD Law MH Lebrun YH Lee I Miyara N Moore U Neumann K Nordström DG Panaccione R Panstruga M Place RH Proctor D Prusky G Rech R Reinhardt JA Rollins S Rounsley CL Schardl DC Schwartz N Shenoy K Shirasu UR Sikhakolli K Stüber 《Nature genetics》2012,44(9):1060-1065