Antisense expression of a rice cellular apoptosis susceptibility gene (OsCAS) alters the height of transgenic rice

Cellular apoptosis susceptibility (CAS) gene plays important roles in mitosis, development and export of importin α from the nucleus, but its function in plant is unknown. In this study, a rice CAS ortholog (OsCAS), which encodes a predicted protein of 983 amino acids with 62% similarity to human CAS, was identified. DNA gel blot analysis revealed a single copy of OsCAS in the rice genome. A 973 bp fragment at the 3′ end of OsCAS cDNA was cloned from rice cDNA library and transferred into rice in the antisense direction under the control of CaMV 35S promoter via Agrobacterium-mediated transformation method, 105 transgenic lines were obtained. Expression of OsCAS was suppressed in the antisense transgenic lines as revealed by semi-quantitative RT-PCR. The antisense transgenic lines showed dwarf phenotypes. The results indicated that OsCAS was involved in culm development of rice.     

泥膜对软土泥水盾构隧道施工的影响

泥水盾构施工中有三种机理对泥膜的形成起作用.泥膜阻止隧道掘进流体自由流入剪切区域,造成泥浆粘度增高和含泥浆的土的渗透性降低.不管泥膜的贯入深度大小,覆盖泥浆的饱和土所需的切削力比无泥浆覆盖的饱和土所需的切削力比无泥浆覆盖的饱和土所需的切削力至少高一倍.气窝也更容易在覆盖泥浆的饱和土内出现.     

Double Star TC-1 observation of the earthward flowing plasmoids in the near magnetotail

We analyze Double Star TC-1 magnetic field data from July to September in 2004 and find that plas-moids exist in the very near-Earth magnetotail. It is the first time that TC-1 observes the plasmoids inthe magnetotail at X > ?13 RE. According to the difference of the magnetic field structure in plasmoids,we choose two typical cases for our study: the magnetic flux rope on August 6 with the open magneticfield and the magnetic loop on September 14 with the closed magnetic field. Both of the cases are as-sociated with the high speed earthward flow and the magnetic loop is related to a strong substorm. Theions can escape from the magnetic flux rope along its open field line, but the case of the closed mag-netic loop can trap the ions. The earthward flowing plasmoids observed by TC-1 indicate that the mul-tiple X-line magnetic reconnection occurs beyond the distance of X=?10 RE from the earth.     

Meridional seesaw-like distribution of the Meiyu rainfall over the Changjiang-Huaihe River Valley and characteristics in the anomalous climate years

Although Meiyu rainfall has its in-phase spatial variability over the Changjiang-Huaihe River Valley (CHRV) in most years, it is distributed in some years like a seesaw to the north and south of the Changjiang River, when the precipitation tends to be nearly normal throughout the valley, which would inevitably increase difficulties of making short-term prediction of the rainfall. For this reason, EOF analysis is made on 15 related stations’ precipitation from June to July during 1951─2004, revealing that the EOF2 mode shows largely a north-south seesaw-like pattern, and thereby classifying Meiyu patterns into two types: "northern drought and southern flood (NDSF)" and "northern flood and southern drought (NFSD)". Afterwards, the authors investigated ocean-atmospheric characteristics when these two anomalous types occured using the NCEP reanalysis (version 1) and the extended reconstructed SSTs (version 2). The results show that in the NDSF years, the low-level frontal area and moisture convergence center lie more southward, accompanied by weaker subtropical summer mon- soon over East Asia, with the western Pacific subtropical high and 200 hPa South Asia High being more southward. Both the Northern and Southern Hemisphere Annular Modes are stronger than normal in preceding February; SST is higher off China during boreal winter and spring and the opposite happens in the NFSD years. Also, this seesaw-form Meiyu rainfall distribution might be affected to some degree by the previous ENSO event.     

Synthesis and characterization of wormhole-like mesoporous Ce0.6Zr0.35Y0.05O2 solid solutions

Nanoparticles of Ce0.6Zr0.35Y0.05O2 (CZY) solid solution have been prepared by the CTAB (hexadecyl-trimethyl ammonium bromide), CTAB-EG (ethylene glycol) templating, and CTAB-EG-NaCl (in which the pores of the precursor synthesized by the CTAB-EG method is filled by a certain amount of NaCl) method, respectively. The physical properties of these materials were characterized by means of tech-niques such as X-ray diffraction (XRD), high resolution scanning electron microscopy (HRSEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), and N2 adsorp-tion-desorption measurements. The CZY samples synthesized by the above three methods display wormhole-like mesoporous morphology and cubic crystal structures. The materials are narrow in pore size distribution (averaged pore diameter = 5.3―7.1 nm), high in surface areas (95―119 m2/g), and large in pore volumes (0.16―0.18 cm3/g). It has been demonstrated that the introduction of NaCl is capable of retaining the pore structures of solid nanomaterials at high-temperature calcination.     

Molecular phylogeny and biogeography of the highly specialized grade schizothoracine fishes （Teleostei： Cyprinidae） inferred from cytochrome b sequences

We recovered the phylogenetic relationships among 23 species and subspecies of the highly special-ized grade schizothoracine fishes distributing at 36 geographical sites in the Tibetan Plateau and its surrounding regions by analyzing sequences of cytochrome b genes. Furthermore, we estimated the possible divergent times among lineages based on a historical geological isolation event in the Tibetan Plateau. The molecular data revealed that the highly specialized grade schizothoracine fishes were not a monophyletic group, but were the same as genera Gymnocypris and Schizogypsis. Our results indi-cated that the molecular phylogenetic relationships apparently reflected their geographical and historical associations with drainages, namely species from the same and adjacent drainages clustered together and had close relationships. The divergence times of different lineages were well consistent with the rapid uplift phases of the Tibetan Plateau in the late Cenozoic, suggesting that the origin and evolution of schizothoracine fishes were strongly influenced by environment changes resulting from the upheaval of the Tibetan Plateau.     

DNA addition using linear self-assembly

This paper presents a DNA algorithm which adds two nonnegative binary integers using self-assembly in constant steps. The approach has the benefit of greater experimental simplicity when compared with previous DNA addition algorithms. For the addition of two binary n-bit integers, O（n） is different from DNA strands and only O（1） biochemical experimental procedures are required.     

Effects of Land Use Change on the Ecosystem Services Value in the Dry-Hot Valley

The objective of the study reported here was to determine whether LANDSAT TM images could be used to quantify changes in land-use and ecosystem services in Yuanmou County. The sizes of six land use/land cover （LUCC） categories were estimated in Yuanmou County according to the LANDSAT TM images in the summer of 1986 and 2005. Coefficients published by Xie Gaodi and co-workers in 2003 were used to value changes in ecosystem services delivered by each land use/land cover category, and the ecosystem services sensitivity analysis was conducted to determine the effect of manipulating these coefficients on the estimated values. The important results are summarized as followings. （1） The estimated size of cultivated land, pasture land, water area and unused land decreased by 6.39%, 1.35%, 2.25% and 10.67% respectively between 1986 and 2005. By contrast, the estimated size of forest land and construction land increased by about 2.23% and 71.15% respectively between 1986 and 2005. （2） The total ecosystem services value （EVS） of the study area increased from 2 142 132 609.46 yuan to 2 146 416 621.00 yuan, with the net increase of 4 284 011.54 yuan during the 20-year time period. （3） The coefficient sensitivity （CS） of the study are less than unity in all cases （CS 〈 1）. This indicates that the total ecosystem values estimated for the study area are relatively inelastic with respect to the ecosystem service coefficients. While this implies that our estimates are robust and the coefficient is reasonable, highly under or over valued coefficients can substantially affect the veracity of estimated changes in ecosystem service values overtime even when the CS are less than unity（CS 〈 1）.     

Effects of deposition parameters on the properties of VO2 thin films

The vanadium oxide thin films are deposited for microbolometers by radio frequency reactive sputtering method at room temperature. The effects of the oxygen partial pressure on the deposition rate, electrical properties and compositions of the films are discussed. The as-deposited VO_x thin films with x value of nearly 2 are deposited by adjusting the oxygen partial pressure. After oxidation annealing of these films in air, the VO₂ films with high temperature coefficients of resistivity (about -4%/℃) and low resistivity can be obtained. The square resistances of the films are in the range of 100 kΩ/squ?300 kΩ/squ. All films are deposited at room temperature and annealed at 400℃, in which the compatibility between VO_x deposition process and MEMS (micro electromechanical systems) is greatly improved.     

Electron field emission from nano-crystalline Si films deposited by inductively coupled plasma CVD at room temperature

ELECTRON FIELD EMISSION IS ONE OF THE INTERESTING PER- FORMANCES OF NANO-SCALE MATERIALS [1-3]. CARBON NANO- TUBES HAVE BEEN DEMONSTRATED TO BE THE MOST FASCINAT- ING MATERIAL OF ELECTRON FIELD EMISSION, BUT THEIR INSTA- BILITY OF EMISSION DUE TO THE AGING EFFECT LIMITS THEIR APPLICATION IN VACUUM ELECTRONICS [4]. SI TIPS HAVE MORE FAVORITE GEOMETRIC FEATURE AND MORE STABLE C…