全文获取类型
收费全文 | 27858篇 |
免费 | 389篇 |
国内免费 | 677篇 |
专业分类
系统科学 | 752篇 |
丛书文集 | 705篇 |
教育与普及 | 500篇 |
理论与方法论 | 87篇 |
现状及发展 | 6283篇 |
研究方法 | 801篇 |
综合类 | 19274篇 |
自然研究 | 522篇 |
出版年
2022年 | 225篇 |
2021年 | 303篇 |
2015年 | 341篇 |
2014年 | 471篇 |
2013年 | 557篇 |
2012年 | 820篇 |
2011年 | 1371篇 |
2010年 | 721篇 |
2009年 | 779篇 |
2008年 | 966篇 |
2007年 | 1044篇 |
2006年 | 968篇 |
2005年 | 848篇 |
2004年 | 675篇 |
2003年 | 719篇 |
2002年 | 924篇 |
2001年 | 1042篇 |
2000年 | 923篇 |
1999年 | 907篇 |
1998年 | 528篇 |
1997年 | 459篇 |
1996年 | 406篇 |
1995年 | 391篇 |
1994年 | 336篇 |
1993年 | 303篇 |
1992年 | 481篇 |
1991年 | 424篇 |
1990年 | 418篇 |
1989年 | 387篇 |
1988年 | 338篇 |
1987年 | 302篇 |
1986年 | 247篇 |
1985年 | 257篇 |
1984年 | 240篇 |
1979年 | 445篇 |
1978年 | 340篇 |
1977年 | 367篇 |
1976年 | 264篇 |
1975年 | 322篇 |
1974年 | 446篇 |
1973年 | 379篇 |
1972年 | 360篇 |
1971年 | 421篇 |
1970年 | 484篇 |
1969年 | 425篇 |
1968年 | 407篇 |
1967年 | 442篇 |
1966年 | 384篇 |
1965年 | 301篇 |
1958年 | 231篇 |
排序方式: 共有10000条查询结果,搜索用时 62 毫秒
51.
To validate procedures of rational drug design, it is important to develop computational methods that predict binding sites between a protein and a ligand molecule. Many small molecules have been tested using such programs, but examination of protein-protein and peptide-protein interactions has been sparse. We were able to test such applications once the structures of both the maltose-binding protein (MBP) and the ligand-binding domain of the aspartate receptor, which binds MBP, became available. Here we predict the binding site of MBP to its receptor using a 'binary docking' technique in which two MBP octapeptide sequences containing mutations that eliminate maltose chemotaxis are independently docked to the receptor. The peptides in the docked solutions superimpose on their original positions in the structure of MBP and allow the formation of an MBP-receptor complex. The consistency of the computational and biological results supports this approach for predicting protein-protein and peptide-protein interactions. 相似文献
52.
Short alanine peptides, containing 16 or 17 residues, appear to form alpha-helices in aqueous solution. But the main spectroscopic analyses used on helical peptides (circular dichroism and nuclear magnetic resonance) cannot distinguish between an alpha-helix (in which the ith residue is hydrogen-bonded to residue i + 4; ref. 9) and the next most common peptide helix, the 3(10)-helix10 (i-->i + 3 hydrogen-bonding). To address this problem we have designed single and doubly spin-labelled analogues of alanine-based peptides in which the nitroxide spin label forms an unbranched side chain extending from the sulphur atom of a cysteine residue. Here we report the circular dichroism, Fourier-transform infrared and electron-spin resonance spectra of these peptides under helix-forming conditions. The infrared absorbance gives an amide I' band with a frequency that is substantially different from that observed for alpha-helices. The electron-spin resonance spectra of doubly labelled helices show that the ranking of distances between side chains, around a single turn (residues 4-8), is inconsistent with an alpha-helical structure. Our experiments suggest that the more likely peptide geometry is a 3(10)-helix. 相似文献
53.
模拟和验证了一种低成本的,以标准CMOS工艺为基础,无需对原工艺流程进行改动的高压工艺技术。讨论了低压器件中的各种击穿机理,相应提出了高压器件中所做出的改进,列举了该工艺技术中所用的特殊版图;对此工艺的应用性进行了二维的工艺和器件模拟;将模拟结果与实际测试结果进行了比较,验证了这种高压工艺技术的实用性。 相似文献
54.
A calcium sensor in the sodium channel modulates cardiac excitability. 总被引:11,自引:0,他引:11
Hanno L Tan Sabina Kupershmidt Rong Zhang Svetlana Stepanovic Dan M Roden Arthur A M Wilde Mark E Anderson Jeffrey R Balser 《Nature》2002,415(6870):442-447
Sodium channels are principal molecular determinants responsible for myocardial conduction and maintenance of the cardiac rhythm. Calcium ions (Ca2+) have a fundamental role in the coupling of cardiac myocyte excitation and contraction, yet mechanisms whereby intracellular Ca2+ may directly modulate Na channel function have yet to be identified. Here we show that calmodulin (CaM), a ubiquitous Ca2+-sensing protein, binds to the carboxy-terminal 'IQ' domain of the human cardiac Na channel (hH1) in a Ca2+-dependent manner. This binding interaction significantly enhances slow inactivation-a channel-gating process linked to life-threatening idiopathic ventricular arrhythmias. Mutations targeted to the IQ domain disrupted CaM binding and eliminated Ca2+/CaM-dependent slow inactivation, whereas the gating effects of Ca2+/CaM were restored by intracellular application of a peptide modelled after the IQ domain. A naturally occurring mutation (A1924T) in the IQ domain altered hH1 function in a manner characteristic of the Brugada arrhythmia syndrome, but at the same time inhibited slow inactivation induced by Ca2+/CaM, yielding a clinically benign (arrhythmia free) phenotype. 相似文献
55.
感应电会对人体造成伤害。从电磁感应和静电感应原理入手,分析了产生感应电的过程,并就如何防止感应电的危害提出了防护对策,有一定实用价值。 相似文献
56.
Abnormal pattern detected in fragile-X patients by pulsed-field gel electrophoresis. 总被引:26,自引:0,他引:26
The fragile-X syndrome is the most frequent inherited form of mental retardation, with an incidence of 1 in 1,500 males. It is characterized by the presence of a fragile site at Xq27.3 induced in vitro by folate deprivation or by inhibitors of deoxynucleotide synthesis. Its mode of inheritance is unusual for an X-linked trait, with incomplete penetrance in both males and females. Some phenotypically normal males transmit the mutation to all their daughters who rarely express any symptoms, but penetrance is high in sons and daughters of these carrier women. Genetic and physical mapping of the Xq27-q28 region has confirmed that the disease locus is located at or very near the fragile site. Hypotheses proposed to account for the abnormalities in the inheritance of the disease include sequence rearrangements by meiotic recombination or a mutation that affects reactivation of an inactive X chromosome during differentiation of female germ cells. To detect such rearrangements, or methylation changes that may reflect a locally inactive X chromosome, we used pulsed-field gel analysis of DNA from fragile-X patients with probes close to the fragile-X locus. The probe Do33 (DXS465) detected abnormal patterns in fragile-X patients, but not in normal controls or in non-expressing male transmitters. 相似文献
57.
介绍了国内外矫直技术与矫直设备的发展水平与方向 ,在简要介绍中厚板矫直机理的基础上 ,指出了目前我国中厚板矫直技术存在的一些差距与不足 ,为我国中厚板矫直设备的改造提供了一定的参考依据。 相似文献
58.
信息技术与学科的有效整合需要教师具备整合的基本能力,并成为研究型教师。指出教师要不断更新信息技术知识和技能,利用现代教学设施,依据教学实践开展研究,从而探索出有效的整合模式。 相似文献
59.
逐次超松弛迭代(SOR)法是求解代数方程组应用较为广泛和有效的方法之一。此文通过对雷诺方程的求解,对SO方法求解精度判据δ和松弛因子ω选取等问题进行若干深入探讨,并通过大量的数值试验对其进行了分析研究。 相似文献
60.
A. Gutnisky P. Glikman M. F. Gimeno A. L. Gimeno 《Cellular and molecular life sciences : CMLS》1982,38(1):116-117
Summary The administration of 15(R)-15-methyl prostaglandin E2 (15(R)-15-M-PGE2) in vivo significantly diminished the uptake of59Fe into blood, spleen, liver, femur and dried intestine of rats, whereas acetylsalicylic acid (ASA) increased the counts significantly. This effect of ASA was counteracted by 15(R)-15-M-PGE2. It is suggested that prostaglandins (PGs) might play an important role in inhibiting iron absorption at the intestinal level.This work was supported by grant No.6638 from CONICET (Argentina). The technical assistance of Mrs María E. Castro and Norma Rizzo is gratefully acknowleged. 相似文献