全文获取类型
收费全文 | 5314篇 |
免费 | 15篇 |
国内免费 | 32篇 |
专业分类
系统科学 | 31篇 |
丛书文集 | 57篇 |
教育与普及 | 4篇 |
理论与方法论 | 19篇 |
现状及发展 | 2617篇 |
研究方法 | 264篇 |
综合类 | 2300篇 |
自然研究 | 69篇 |
出版年
2017年 | 34篇 |
2012年 | 77篇 |
2011年 | 151篇 |
2008年 | 110篇 |
2007年 | 131篇 |
2006年 | 116篇 |
2005年 | 105篇 |
2004年 | 109篇 |
2003年 | 92篇 |
2002年 | 112篇 |
2001年 | 156篇 |
2000年 | 173篇 |
1999年 | 104篇 |
1992年 | 91篇 |
1991年 | 71篇 |
1990年 | 70篇 |
1989年 | 64篇 |
1988年 | 51篇 |
1987年 | 70篇 |
1986年 | 69篇 |
1985年 | 127篇 |
1984年 | 76篇 |
1983年 | 68篇 |
1982年 | 60篇 |
1981年 | 61篇 |
1980年 | 69篇 |
1979年 | 131篇 |
1978年 | 124篇 |
1977年 | 133篇 |
1976年 | 121篇 |
1975年 | 134篇 |
1974年 | 145篇 |
1973年 | 110篇 |
1972年 | 102篇 |
1971年 | 149篇 |
1970年 | 229篇 |
1969年 | 151篇 |
1968年 | 138篇 |
1967年 | 163篇 |
1966年 | 124篇 |
1965年 | 82篇 |
1964年 | 48篇 |
1962年 | 37篇 |
1959年 | 67篇 |
1958年 | 82篇 |
1957年 | 81篇 |
1956年 | 52篇 |
1955年 | 48篇 |
1954年 | 54篇 |
1948年 | 41篇 |
排序方式: 共有5361条查询结果,搜索用时 31 毫秒
441.
M. Gumińska M. B. Stachurska B. Christensen V. Tromholt J. Kieler Cz. Radzikowski D. Duś 《Cellular and molecular life sciences : CMLS》1989,45(6):571-574
Summary It was found that a decrease in electrophoretic mobility of pyruvate kinase (PK) isoenzyme, and an increase of the sensitivity of this enzyme to L-cysteine, were markers of immortalization and tumorigenic properties, respectively, in human urothelial cell lines characterized by different grades of transformation (TGr) in vitro. 相似文献
442.
A sex pheromone produced by male cabbage looper moths, Trichoplusia ni (Hübner), has been isolated from the genital scent brushes and identified as 2-phenylethanol. It is shown conclusively to elicit specific behavioural responses in the female (such as wing vibration and abdominal elevation), as determined by a novel behavioural laboratory bioassay. This is taken as further evidence that the male pheromone of T. ni acts as a sexual stimulant (aphrodisiac) prior to mating. 2-Phenylethanol represents the first identification of a genital scent brush pheromone in the family Noctuidae, and of a male pheromone in the subfamily Plusiinae. 相似文献
443.
444.
The ability of dog renal cortex slices to accumulate beta-methyl-glucoside or glycine is enhanced by the flavonoid (+)-catechin at a concentration of 3.5 mM. This stimulatory effect is apparently due to a decreased rate of efflux of either substrate. On the other hand, the uptake of p-amino-hippuric acid and N1-methyl-nicotinamide is inhibited by (+)-catechin. The drug at the same concentration is without action on amino-acid transport by guinea-pig intestine in vitro. 相似文献
445.
446.
Distribution of calcitonin cells in the thyroid glands of normal adult rhesus monkey Macaca mulatta.
The calcitonin cells of adult rhesus monkey Macaca mulatta are located in the central region of thyroid along the median axis. The anterior and posterior poles, the isthmus and peripheral regions of thyroid are completely devoid of C cells. The parathyroid also lacks C cells. 相似文献
447.
The colour centre in the cerebral cortex of man 总被引:23,自引:0,他引:23
C J Lueck S Zeki K J Friston M P Deiber P Cope V J Cunningham A A Lammertsma C Kennard R S Frackowiak 《Nature》1989,340(6232):386-389
Anatomical and physiological studies have shown that there is an area specialized for the processing of colour (area V4) in the prestriate cortex of macaque monkey brain. Earlier this century, suggestive clinical evidence for a colour centre in the brain of man was dismissed because of the association of other visual defects with the defects in colour vision. However, since the demonstration of functional specialization in the macaque cortex, the question of a colour centre in man has been reinvestigated, based on patients with similar lesions in the visual cortex. In order to study the colour centre in normal human subjects, we used the technique of positron emission tomography (PET), which measures increases in blood flow resulting from increased activity in the cerebral cortex. A comparison of the results of PET scans of subjects viewing multi-coloured and black-and-white displays has identified a region of normal human cerebral cortex specialized for colour vision. 相似文献
448.
Summary Two new C-18-oxygenated sterols possessing a spiroketal function (1 and2) have been isolated from a soft coral of the genusSinularia. The proposed structures are based on spectral data. 相似文献
449.
Cryopreservation of Drosophila melanogaster embryos 总被引:3,自引:0,他引:3
P L Steponkus S P Myers D V Lynch L Gardner V Bronshteyn S P Leibo W F Rall R E Pitt T T Lin R J MacIntyre 《Nature》1990,345(6271):170-172
There is an urgent need to preserve the ever-increasing number (greater than 30,000) of different genetic strains of D. melanogaster that are maintained in national and international stock centres and in the laboratories of individual investigators. In all cases, the stocks are maintained as adult populations and require transfer to fresh medium every two to four weeks. This is not only costly in terms of materials, labour and space, but unique strains are vulnerable to accidental loss, contamination, and changes in genotype that can occur during continuous culture through mutation, genetic drift or selection. Although cryopreservation of Drosophila germ-plasm would be an enormous advantage, many attempts using conventional procedures have been unsuccessful. D. melanogaster embryos are refractory to conventional cryopreservation procedures because of the contravening conditions required to minimize mortality resulting from both intracellular ice formation and chilling injury at subzero temperatures. To overcome these obstacles, we have developed a vitrification procedure that precludes intracellular ice formation so that the embryos can be cooled and warmed at ultra-rapid rates to minimize chilling injury, and have recovered viable embryos following storage in liquid nitrogen. In a series of 53 experiments, a total of 3,711 larvae emerged from 17,280 eggs that were cooled in liquid nitrogen (18.4 +/- 8.8%). Further, using a subset from this population, approximately 3% of the surviving larvae (24/800) developed into adults. These adults were fertile and produced an F1 generation. 相似文献
450.
Telomere-telomere recombination provides an express pathway for telomere acquisition 总被引:32,自引:0,他引:32
DNA termini from Tetrahymena and Oxytricha, which bear C4A2 and C4A4 repeats respectively, can support telomere formation in Saccharomyces cerevisiae by serving as substrates for the addition of yeast telomeric C1-3A repeats. Previously, we showed that linear plasmids with 108 base pairs of C4A4 DNA (YLp108CA) efficiently acquired telomeres, whereas plasmids containing 28-64 base pairs of C4A4 DNA also promoted telomere formation, but with reduced efficiency. Although many of the C4A4 termini on these plasmids underwent recombination with a C4A2 terminus, the mechanism of telomere-telomere recombination was not established. We now report the sequence of the C4A4 ends from the linear plasmids. The results provide strong evidence for a novel recombination process involving a gene conversion event that requires little homology, occurs at or near the boundary of telomeric and nontelomeric DNA, and resembles the recombination process involved in bacteriophage T4 DNA replication. 相似文献