Effect of hydrogen peroxide on the binding of Merocyanine 540 to human erythrocytes

The fluorescent dye Merocyanine 540 (MC540) is often used as a probe to monitor the molecular packing of phospholipids in the outer leaflet of biomembranes. In a previous study we showed that the increased staining of erythrocytes with a perturbed membrane structure was mainly due to an increase in the fluorescence yield of cell-bound MC540, rather than to an increase of the number of bound molecules. Erythrocytes and ghosts exposed to continuous fluxes of H₂O₂ exhibited pronounced lipid peroxidation. Further, red blood cells subjected to this form of oxidative stress also showed increased staining with MC540. It appeared that this was caused by a strong increase in binding of MC540, together with a slight red shift of the fluorescence emission maximum and a small increase in the fluorescence yield of bound MC540. The changed MC540 binding characteristics were not observed when lipid peroxidation was suppressed by the presence of the antioxidant BHT in the incubation medium. However, open ghosts exposed to H₂O₂ showed no increase of MC540 binding, excluding a direct involvement of lipid peroxidation. Measurement of fluorescence emission spectra and gel filtration studies showed that MC540 can bind to H₂O₂-exposed hemoglobin. Experiments with erythrocytes lysed in hypotonic medium after exposure to H₂O₂ revealed that peroxidation of lipids with H₂O₂ induced a non-specific permeabilization of the plasma membrane to MC540, thereby allowing MC540 to bind to the oxidatively denatured, more hydrophobic hemoglobin. These results indicate that conclusions about packing of phospholipids in the outer leaflet of the membrane based on increased MC540-staining should be drawn with care. Received 27 September 1996; received after revision 5 November 1996; accepted 27 November 1996     

平台用钢微观组织对其分层面上解理断裂应力的影响

利用专门设计的厚度方向缺口四点弯曲试样，结合相应的弹塑性有限元计算结果，在液氮温度下测取了一种海洋平台用钢的６种微观组织分层面上的解理断裂应力σ＊ｆ．结果显示，均匀细小的铁素体＋贝氏体组织具有比铁素体＋带状珠光体为高的σ＊ｆ值．对于同一终轧温度，轧后冷速愈高，其得到的微观组织之σ＊ｆ值愈高．而对于同一轧后冷却制度，则终轧于Ａｒ３与再结晶停止温度之间者其得到的微观组织具有最高的σ＊ｆ值．讨论了各微观组织组成相的作用     

幂律流体不稳定径向流的数值解法

对微可压缩非牛顿幂律流体在均质多孔介质中的非稳定径向渗流方程进行了回顾和对比，用有限差分方法建立了微分方程的数值解法．研究了时间步长、空间步长对数值解精度和稳定性的影响，并结合算例绘出了不同流性指数的瞬时压力曲线．该研究结果可用于非牛顿流体渗流机理研究及试井分析．     

随机冰载作用下海上固定平台管节点的疲劳寿命估算

根据冰激振动理论，分析了海冰对海上钻井平台的不同破坏形式．为了解决海洋石油钢结构在低温冰载作用下的疲劳性能问题，进行了室温与低温下随机冰载作用对钢结构疲劳裂纹扩展的试验研究．根据试验结果，计算了平台管节点的疲劳累积损伤和随机冰载的等效应力，提出了综合计算等效应力幅的新方法，给出了管节点的疲劳寿命曲线及其计算参数．这种新的计算方法，考虑了冰载相对时间和相对冰厚的随机性，能提高寿命估算的精确度     

微型裂解法评价乙烯原料技术

对一种可在生产现场使用的简易快速的裂解原料评价技术进行了综合评述．其原理是将最大限度微型化了的裂解炉与气相色谱仪串联或并联，采用脉冲进料，裂解后的有用产物全部进入色谱仪进行分析．调整微型裂解炉的反应条件，可以使裂解产物分布十分接近工业炉的结果，可分析预测Ｃ１～Ｃ４全部气体产物的工业炉产率、Ｃ６～Ｃ８芳烃工业炉产率和原料的相对结焦趋势．所消耗的人力、物力和时间少于原料性质的常规分析，预测成本仅为模拟试验炉的１／１０．该技术还可用于裂解基础研究．     

不同介质PCVD涂层对结构钢耐磨性的影响

旨在探索不同介质的ＰＣＶＤ涂层对结构钢表面硬度及耐磨性的影响。实验采用两种不同的金属有机溶液（（Ｃ３Ｈ７Ｏ）４Ｔｉ；（ＣＨ３）６ＳｉＮ）对４２ＣｒＭｏ钢进行表面处理。通过测量显微硬度及耐磨试验，得出以下结果：钛酸丙脂处理，其表面硬度及耐磨性均较好；而氮－硅烷处理，虽表面硬度较高，但耐磨性较差。这两种介质的表面处理都优于常规的离子渗氮     

熔盐Cr-Ti碳化物复合层涂覆技术

对表面镀铬、渗铬的钢件进行硼砂熔盐浸渍渗钛处理，可形成多元碳化物复合层。其抗冲击疲劳及抗介质腐蚀性能均明显优于单一渗钛层。应用Ｘ－ｒａｙ衍射及电子探针技术，确定了复合层的结构。     

Spectrum characteristics and pump wavelengths of Nd3+-doped double-clad fiber

Conclusion The optimum wavelength of Nd³⁺-doped double-clad fiber laser or amplifier made in China is near 830 nm. The efficiency of Nd³⁺-doped double-clad fiber devices pumped by 820-nm laser is low due to ESA. In order to increase the photochemical stability of the outer cladding, the material of the outer cladding must be advanced.