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471.
The essential light chains constitute part of the active site of smooth muscle myosin 总被引:5,自引:0,他引:5
Myosin, a major contractile protein, characteristically possesses a long coiled-coil alpha-helical tail and two heads. Each head contains both an actin binding site and an ATPase site and is formed from the NH2-terminal half of one of the two heavy chains (relative molecular mass, Mr, 200,000) and a pair of light chains; the so-called regulatory and essential light chains of approximately Mr 20,000 each. Recently we have identified Trp 130 of the myosin heavy chain from rabbit skeletal muscle as an active-site amino-acid residue after labelling with a new photoaffinity analogue of ADP, N-(4-azido-2-nitrophenyl)-2-aminoethyl diphosphate (NANDP). Nonspecific labelling was eliminated by first trapping NANDP at the active site with thiol crosslinking agents. Exclusive labelling of the heavy chains with no labelling of the light chains agreed with previous findings that the heavy chains alone contain the actin-activated Mg-ATPase activity of rabbit skeletal myosin. Here we report similar photolabelling experiments with smooth muscle myosin (chicken gizzard) in which 3H-NANDP is trapped at the active site with vanadate and which show that both the heavy chains and the essential light chains are labelled. The results indicate that both chains contribute to the ATP binding site and represent the first direct evidence for participation of the essential light chains in the active site of any type of myosin. 相似文献
472.
There is now good evidence that cytoplasmic pH (pHi) may have an important role in the metabolic activation of quiescent cells. In particular, growth stimulation of mammalian fibroblasts leads to a rapid increase in pHi (refs 3-6), due to activation of a Na+/H+ exchanger in the plasma membrane, and this alkalinization is necessary for the initiation of DNA synthesis. However, the mechanism by which mitogens activate the Na+/H+ exchanger to raise pHi is not known, although an increase in cytoplasmic free Ca2+ ([Ca2+]i) has been postulated as the primary trigger. We now present data suggesting that the Na+/H+ exchanger is set in motion through protein kinase C, a phospholipid- and Ca2+-dependent enzyme normally activated by diacylglycerol produced from inositol phospholipids in response to external stimuli. Using newly developed pH microelectrodes and fluorimetric techniques, we show that a tumour promoting phorbol ester and synthetic diacylglycerol, both potent activators of kinase C (refs 12-15), mimic the action of mitogens in rapidly elevating pHi in different cell types. Furthermore, we demonstrate that, contrary to previous views, an early rise in [Ca2+]i is not essential for the activation of Na+/H+ exchange and the resultant increase in pHi. Finally, we suggest that an alkaline pHi shift, mediated by Na+/H+ exchange, may be a common signal in the action of those hormones which elicit the breakdown of inositol phospholipids. 相似文献
473.
G. Planz J. Bieber H. J. Mencke G. v. Bernuth 《Cellular and molecular life sciences : CMLS》1983,39(5):497-498
Summary Plasma catecholamine concentrations in 46 children of various ages were determined by a sensitive radioenzymatic assay. Noradrenaline levels were found to be in the same range as in adults, whereas adrenaline levels in a few of the children were abnormally high. 相似文献
474.
Molecular structure of r(GCG)d(TATACGC): a DNA--RNA hybrid helix joined to double helical DNA 总被引:20,自引:0,他引:20
A H Wang S Fujii J H van Boom G A van der Marel S A van Boeckel A Rich 《Nature》1982,299(5884):601-604
The molecule r(GCG)d(TATACGC) is self-complementary and forms two DNA--RNA hybrid segments surrounding a central region of double helical DNA; its molecular structure has been solved by X-ray analysis. All three parts of the molecule adopt a conformation which is close to that seen in the 11-fold RNA double helix. The conformation of the ribonucleotides is partly determined by water molecules bridging between the ribose O2' hydroxyl group and cytosine O2. The hybrid-DNA duplex junction contains no structural discontinuities. However, the central DNA TATA sequence has some structural irregularities. 相似文献
475.
J. B. Gibson A. V. Wilks G. K. Chambers 《Cellular and molecular life sciences : CMLS》1982,38(6):653-654
Summary A 3rd allele at theAdh locus,Adh
FCh.D., has been found at polymorphic frequencies in natural populations ofD. melanogaster. The ADH-FChD enzyme has properties distinct from those of the 2 more common forms of ADH. TheAdh polymorphism should now be analyzed as a triallelic system. 相似文献
476.
477.
Summary Stimulation of aminopyrine demethylation induced in rats by oral or i.p. administration of phenobarbital was partially inhibited in animals receiving daily treatments of 2×200 mg/kg gum Arabic p.o. 相似文献
478.
G Barchewitz F Binon A Cier H Tuchmann-Duplessis 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1980,291(4):409-411
When orally administered, 5-(3-diméthylamino propyliden)-benzofuro [2,3-c] benzoxepin-1, which antagonizes several biogenic amines, is slightly sedative and has also endocrinological properties in the Rat, induces the appearance of localized lesions of uterine stroma in 2 to 16% of the animals. The cytological picture could suggest a tumorogenesis. In fact, these lesions represent a non-tumoral reactional hyperplasia with nuclear abnormalities due to a hormonal stimulation. 相似文献
479.
Effect of conditioned media on nerve cell differentiation 总被引:2,自引:0,他引:2
M. Sensenbrenner G. G. Jaros G. Moonen B. J. Meyer 《Cellular and molecular life sciences : CMLS》1980,36(6):660-662
Summary Glial conditioned medium strongly stimulates the morphological maturation of cultured neuronal cells, while fibroblast and meningeal conditioned media have weaker effects.Acknowledgment. We acknowledge the expert technical assistance of Mrs M.F. Knoetgen. 相似文献
480.
G. H. Rossmanith J. Unsworth R. D. Bell 《Cellular and molecular life sciences : CMLS》1980,36(1):51-53
Summary Small-amplitude sinusoidal displacements, in the frequency rang 4–100 Hz, were applied to intact whole frog sartorius muscle whilst in a state of tetanus. At low frequencies the muscle, was observed to do oscillatory work, while at higher frequencies it tended towards elastic behaviour. Frequency-response plots obtained were compared with those from other muscle preparations. Results were interpreted in terms of mechano-chemical transduction properties of muscle.The authors wish to thank the Australian Research Grants Commission for provision of the laser, and L. I. Patterson for construction of the mechanical apparatus. 相似文献