Circadian rhythm of bile secretion in the rat.

In rats the bile flow and the estimated bile acid independant flow (BAIF) were significantly lower at 17.00 h than at 08.00 and 24.00 h. The decrease in BAIF paralleled the decrease in liver weight. Bile acid excretion was not different.     

Stimulation of Autonomic Nerves to thie urinary bladder of the rat

Zusammenfassung In der Harnblase von Ratten wurde der Druck während einseitiger und zweisceitiger Pelvicuserizung gemessen. Nach Injektion von Eserin wurde bereits bei einseitiger Reizung eine maximale Blasenkontraktion erhalten. Zusätzlike Reizung der Nn. hypogastrici führt-ohne funktonelle Überlappungzu weiterer Kontraktionssteigerung.

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This work was supported by grants from the Medical Faculty, University of Lund.     

Effects of mistletoe (Viscum album L.) extracts on cultured tumor cells

Bacterially fermented mistletoe preparations (BFMP) were tested on rat hepatoma tissue culture (HTC) cells and human leukemia Molt 4 cells. A dose-dependent inhibition of the growth rate of the cells was observed. For both cell lines, cytostatic concentrations, expressed in weight of fresh plant, were 0.5 mg/ml culture medium for oak BFMP and 1 mg/ml for apple tree BFMP. However, the action of the two preparations was markedly different on each cell line. Non-viable HTC cells were not stained by trypan blue while non-viable Molt 4 cells were fully colored by this reagent. A lysis of cellular membranes of HTC cells was observed by electron microscopy. Furthermore, oak BFMP inhibited the growth of virus transformed 3T3-SV40 cells more than that of non-transformed 3T3 cells. In contrast to BFMP, non-fermented extracts and a purified mistletoe lectin showed a greater inhibition of the growth of Molt 4 cells than of HTC cells. Samples withdrawn at different times during fermentation gradually lost their inhibitory effect on the growth of Molt 4 cells while their action on HTC cells increased up to the 4th day of fermentation. These results are discussed in relation to the cytotoxic substances of mistletoe already characterized.     

Purification de la S-adénosyl-L-homocystéine hydrolase du foie de rat par chromatographie d'affinité

Summary S-adenosyl-L-homocysteine hydrolase (EC 3.3.1.1) has been purified 240-fold from rat liver by affinity column chromatography on aminohexyl sepharose bound 6-mercaptopurine 9 D-riboside. The purified enzyme was homogeneous by gel electrophoresis.

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Ce travail a bénéficié de l'aide du CNRS (ERA 560) et de l'INSERM (FRA 5).