Cytotoxicity of new cytochalasans fromChaetomium globosum

Zusammenfassung Nachweis, dass CHCl₃-Rohextrakte aus Myzelien und Filtraten der Zuchtmedien vonChaetomium globosum Kunze ex Fries einen akuten toxischen Effect auf HeLa-Zellen in vitro, sowie auf Mäuse zeigten. Chaetoglobosine die aus Mycelium-Rohextrakt isoliert wurden und chemisch zu den Cytochalasanen gehören, induzierten in HeLa-Zellen eine Polynukleierung und multipolare Kernverteilung. Auch beim Chaetogobosin wird eine Mikrofilament-Schädigung angenommen.     

Differentiation antigens in keratinocytes]

By means of indirect immunofluorescence with human sera it has been demonstrated that cytoplasmic components of the basal cell layer showed antigenic differences as compared to the upper cell layers. Moreover there were differences whether the cells were involved in a mucous, a parakeratinized or an orthokeratinized differentiation.     

Immunochemical identification of vitellogenin in the serum of the newtTriturus cristatus

Summary The hematic yolk precursor — vitellogenin — has been identified immunochemically in the serum of estrogenized females of the newtTriturus cristatus by employing an antiserum prepared against yolk proteins.     

HLA-restricted T-cell recognition of Epstein-Barr virus-infected B cells

In mice the cytotoxic T-cell response to several types of virus is influenced by genes within the major histocompatibility complex; in particular, genetic control is exercised at the effector cell level through a requirement that virus-specific cytotoxic T cells recognise viral antigens in association with H-2K and H=2D region gene products on the surface of infected cells. In man the restriction which the analogous HLA-A, -B and -C-region gene products might place on virus-specific T-cell function is still in dispute. The earliest and most controversial evidence concerns the Epstein-Barr virus (EBV), a B lymphotropic agent which causes infectious mononucleosis (IM) and which induces an unusually vigorous T-cell response; cytotoxic T cells from IM patients' blood were shown to be EBV-specific yet, in contrast to mouse systems, apparently free of any obvious HLA restriction. Since then T-cell recognition of EBV-infected B cells has assumed particular significance as a model system for the study of cytotoxic T-cell function in man. This report describes the results of a new approach clearly indicating that HLA-A and -B region products do indeed have a role in this system.     

Effect of lesions of the locus coeruleus complex on the circadian rhythm of plasma corticosterone in the mouse

Summary An apparently transient elevation of basal morning (08.00 h) plasma corticosterone levels in male mice was found 48 h after bilateral electrolytic lesions of the brainstem locus coeruleus complex but was not observed 6 weeks after lesioning.Supported by Alfred P. Sloan Foundation research fellowships awarded to W.G. Luttge and S.F. Zornetzer.     

The effects of oxamate on the contractility of isolated rat atria

Zusammenfassung Oxamat, das die Lactat-Dehydrogenase hemmt, erniedrigt die Kontraktionsfähigkeit des Vorhofs mit und ohne Anwesenheit von Glucose im Medium und bei Hinzufügung von 2-DG oder Atropin. Hohe Konzentration von Pyruvat erhöht die Depression von Oxamat auf den Vorhof im Glucose-Milieu. Oxamat sensibilisiert das Gewebe für funktionellen Abbau durch Glycolyse.

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This research was supported by grants No. 1441 and No. HE-994801 from Consejo Nacional de Investigaciones de la República Argentina, and from the National Institute of Health, respectively.

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Research Fellow from the Consejo Nacional de Investigaciones de la República Argentina.

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Established Investigator from the Consejo Nacional de Investigaciones de la República Argentina.