Approaches to identify and characterize microProteins and their potential uses in biotechnology

MicroProteins are small proteins that contain a single protein domain and are related to larger, often multi-domain proteins. At the molecular level, microProteins act by interfering with the formation of higher order protein complexes. In the past years, several microProteins have been identified in plants and animals that strongly influence biological processes. Due to their ability to act as dominant regulators in a targeted manner, microProteins have a high potential for biotechnological use. In this review, we present different ways in which microProteins are generated and we elaborate on techniques used to identify and characterize them. Finally, we give an outlook on possible applications in biotechnology.     

First Pleistocene jumping mouse ( Zapus , Zapodinae, Rodentia) from Utah

Two of the Little Dell Dam fossil localities produced the 1st Pleistocene records of the jumping mouse Zapus from Utah. We describe these teeth in detail and compare their morphology with both extinct and extant jumping mouse taxa. Although it is not possible to confidently assign these specimens to a particular species, the Little Dell Dam fossils are clearly distinct from the only living jumping mouse ( Zapus princeps ) currently known from Utah. The paracone is attached to the rest of the occlusal surface of the upper 1st and 2nd molars in modern Z. princeps from Utah; the paracone is isolated in the molars from Little Dell Dam. The fossils from Little Dell Dam are the 1st reported records of Pleistocene Zapus west of the Rocky Mountains.     

Base excision repair and design of small molecule inhibitors of human DNA polymerase β

Base excision repair (BER) can protect a cell after endogenous or exogenous genotoxic stress, and a deficiency in BER can render a cell hypersensitive to stress-induced apoptotic and necrotic cell death, mutagenesis, and chromosomal rearrangements. However, understanding of the mammalian BER system is not yet complete as it is extraordinarily complex and has many back-up processes that complement a deficiency in any one step. Due of this lack of information, we are unable to make accurate predictions on therapeutic approaches targeting BER. A deeper understanding of BER will eventually allow us to conduct more meaningful clinical interventions. In this review, we will cover historical and recent information on mammalian BER and DNA polymerase β and discuss approaches toward development and use of small molecule inhibitors to manipulate BER. With apologies to others, we will emphasize results obtained in our laboratory and those of our collaborators.     

Surfing the Third Wave: Experiential Reflections on New Working Practices

Systemic Practice and Action Research - This paper deals with issues and presents changes in practices relating to the new working as realized in the developing e-working world. The paper begins by...     

Sequence of the human insulin gene

The human insulin gene contains two intervening sequences, one is within the region transcribed into the 5'-untranslated segment of the mRNA and the other interrupts the C-peptide encoding region. A comparison of the human with the rat insulin genes indicates potential regulatory regions in the DNA segment preceding the gene and suggests that the ancestral form of the insulin gene had two intervening sequences.     

Learning with participatory monitoring and evaluation in Dir district,northwest frontier province,Pakistan

This paper is concerned with the development of a monitoring and evaluation system for the second phase of a United Nations Drug Control Programme (UNDCP) in Pakistan and the manner in which participatory structures are developed and built on in a complex development context. Initially the paper introduces the context. The project is concerned with rural development issues as part of a structured programme to substitute opium poppy growing with a range of agricultural and social innovations. During the first phase of the project (up to 1993) the main focus of attention for the project was measured in terms of physical progress with road building, electricity supply, irrigation works and concentration on improved agricultural practices. Phase II has set out to build on this, most specifically in terms of gaining the greater participation of local people in the project activity. The paper describes the monitoring and evaluation (M&E) structure for the project and notes that it is intended that M&E further encourage participation and beneficiary analysis (analysis of project benefits by those who are intended to derive most from the project outputs). One of the primary means set out for achieving this was by inducing the professional staff working in the project to adopt new mind sets, adjusting their focus from being technical experts supplying undoubted technical improvements to being co-operative partners, bringing ideas to the area but equally being willing to work through these ideas and their implications and learn from the communities. The process for the development of the monitoring system is described, and the methodology ‘TeamUp’ discussed. Links are made to related areas of study—most specifically the literature of Rapid and Participatory Rural Development (RRA and PRA). Finally, the paper discusses the results of the research to date and the likely extension of the ideas to other projects.     

Book Review: Rational Analysis for a Problematic World Revisited: Problem Structuring Methods for Complexity,Uncertainty, and Conflict. Jonathan Rosenhead and John Mingers. Wiley,Chichester, United Kingdom. 2001, 365 pp., Chaps. 15, £22.50 (Amazon)

Systemic Practice and Action Research -