纳米级超显微硬度法对镉变形行为的研究

利用纳米级超显微硬度压痕法研究了具有六方密排结构纯Ｃｄ(９９９９％)的应力应变及加工硬化行为·根据载荷压痕贯穿深度的测量,证明了在Ｃｄ的压痕变形中存在弹性、弹塑性和完全塑性三个阶段,与每个阶段相对应在其载荷压痕深度曲线上都出现一个斜率的改变,从而可以近似确定金属开始塑性变形的应力范围·其加工硬化指数是随着施加的最大载荷由５ｍＮ时的０１增加到４０ｍＮ时的０３,这主要是和加载的速度有关并满足线性方程·     

Elucidating the principles of the molecular organization of heteropolymeric tight junction strands

Paracellular barrier properties of tissues are mainly determined by the composition of claudin heteropolymers. To analyze the molecular organization of tight junctions (TJ), we investigated the ability of claudins (Cld) to form homo- and heteromers. Cld1, -2, -3, -5, and -12 expressed in cerebral barriers were investigated. TJ-strands were reconstituted by claudin-transfection of HEK293-cells. cis-Interactions and/or spatial proximity were analyzed by fluorescence resonance energy transfer inside and outside of strands and ranked: Cld5/Cld5?>?Cld5/Cld1?>?Cld3/Cld1?>?Cld3/Cld3?>?Cld3/Cld5, no Cld3/Cld2. Classic Cld1, -3, and -5 but not non-classic Cld12 showed homophilic trans-interaction. Freeze-fracture electron microscopy revealed that, in contrast to classic claudins, YFP-tagged Cld12 does not form homopolymers. Heterophilic trans-interactions were analyzed in cocultures of differently monotransfected cells. trans-Interaction of Cld3/Cld5 was less pronounced than that of Cld3/Cld1, Cld5/Cld1, Cld5/Cld5 or Cld3/Cld3. The barrier function of reconstituted TJ-strands was demonstrated by a novel imaging assay. A model of the molecular organization of TJ was generated.     

The Forecasting Performance of a Finite Mixture Regime‐Switching Model for Daily Electricity Prices

Forecasting prices in electricity markets is a crucial activity for both risk management and asset optimization. Intra‐day power prices have a fine structure and are driven by an interaction of fundamental, behavioural and stochastic factors. Furthermore, there are reasons to expect the functional forms of price formation to be nonlinear in these factors and therefore specifying forecasting models that perform well out‐of‐sample is methodologically challenging. Markov regime switching has been widely advocated to capture some aspects of the nonlinearity, but it may suffer from overfitting and unobservability in the underlying states. In this paper we compare several extensions and alternative regime‐switching formulations, including logistic specifications of the underlying states, logistic smooth transition and finite mixture regression. The finite mixture approach to regime switching performs well in an extensive, out‐of‐sample forecasting comparison. Copyright © 2014 John Wiley & Sons, Ltd.     

Disruption of P2RY5, an orphan G protein-coupled receptor, underlies autosomal recessive woolly hair

The genetic determinants of hair texture in humans are largely unknown. Several human syndromes exist in which woolly hair comprises a part of the phenotype; however, simple autosomal recessive inheritance of isolated woolly hair has only rarely been reported. To identify a gene involved in controlling hair texture, we performed genetic linkage analysis in six families of Pakistani origin with autosomal recessive woolly hair (ARWH; OMIM 278150). All six families showed linkage to chromosome 13q14.2-14.3 (Z = 17.97). In all cases, we discovered pathogenic mutations in P2RY5, which encodes a G protein-coupled receptor and is a nested gene residing within intron 17 of the retinoblastoma 1 (RB1) gene. P2RY5 is expressed in both Henle's and Huxley's layers of the inner root sheath of the hair follicle. Our findings indicate that disruption of P2RY5 underlies ARWH and, more broadly, uncover a new gene involved in determining hair texture in humans.     

The mouse X chromosome is enriched for multicopy testis genes showing postmeiotic expression

According to the prevailing view, mammalian X chromosomes are enriched in spermatogenesis genes expressed before meiosis and deficient in spermatogenesis genes expressed after meiosis. The paucity of postmeiotic genes on the X chromosome has been interpreted as a consequence of meiotic sex chromosome inactivation (MSCI)--the complete silencing of genes on the XY bivalent at meiotic prophase. Recent studies have concluded that MSCI-initiated silencing persists beyond meiosis and that most genes on the X chromosome remain repressed in round spermatids. Here, we report that 33 multicopy gene families, representing approximately 273 mouse X-linked genes, are expressed in the testis and that this expression is predominantly in postmeiotic cells. RNA FISH and microarray analysis show that the maintenance of X chromosome postmeiotic repression is incomplete. Furthermore, X-linked multicopy genes exhibit a similar degree of expression as autosomal genes. Thus, not only is the mouse X chromosome enriched for spermatogenesis genes functioning before meiosis, but in addition, approximately 18% of mouse X-linked genes are expressed in postmeiotic cells.     

Sequence variants in the autophagy gene IRGM and multiple other replicating loci contribute to Crohn's disease susceptibility

A genome-wide association scan in individuals with Crohn's disease by the Wellcome Trust Case Control Consortium detected strong association at four novel loci. We tested 37 SNPs from these and other loci for association in an independent case-control sample. We obtained replication for the autophagy-inducing IRGM gene on chromosome 5q33.1 (replication P = 6.6 x 10(-4), combined P = 2.1 x 10(-10)) and for nine other loci, including NKX2-3, PTPN2 and gene deserts on chromosomes 1q and 5p13.     

Historicity and explanation

Some scientific explanations are distinctively historical. The aim of this paper is to say what gives such explanations their historical character. A secondary aim is to describe what makes an explanation a stronger or weaker historical explanation. We begin with a critical discussion of John Beatty's and Eric Desjardins' work on historicity and historical narrative. We then offer an alternative account of historical explanation that draws on the work of earlier philosophers (Gallie, Danto, Mink, and Hull). In that alternative account, we highlight four features of narrative explanation that Beatty and Desjardins underemphasize: central subjects; historical trajectories; the idea that historical narratives are known retrospectively; and criteria for determining what is a stronger or weaker historical narrative.     

Negligible glacial-interglacial variation in continental chemical weathering rates

Chemical weathering of the continents is central to the regulation of atmospheric carbon dioxide concentrations, and hence global climate. On million-year timescales silicate weathering leads to the draw-down of carbon dioxide, and on millennial timescales chemical weathering affects the calcium carbonate saturation state of the oceans and hence their uptake of carbon dioxide. However, variations in chemical weathering rates over glacial-interglacial cycles remain uncertain. During glacial periods, cold and dry conditions reduce the rate of chemical weathering, but intense physical weathering and the exposure of carbonates on continental shelves due to low sea levels may increase this rate. Here we present high-resolution records of the lead isotope composition of ferromanganese crusts from the North Atlantic Ocean that cover the past 550,000 years. Combining these records with a simple quantitative model of changes in the lead isotope composition of the deep North Atlantic Ocean in response to chemical weathering, we find that chemical weathering rates were two to three times lower in the glaciated interior of the North Atlantic Region during glacial periods than during the intervening interglacial periods. This decrease roughly balances the increase in chemical weathering caused by the exposure of continental shelves, indicating that chemical weathering rates remained relatively constant on glacial-interglacial timescales. On timescales of more than a million years, however, we suggest that enhanced weathering of silicate glacial sediments during interglacial periods results in a net draw-down of atmospheric carbon dioxide, creating a positive feedback on global climate that, once initiated, promotes cooling and further glaciation.