Two levels of protection for the B cell genome during somatic hypermutation

Somatic hypermutation introduces point mutations into immunoglobulin genes in germinal centre B cells during an immune response. The reaction is initiated by cytosine deamination by the activation-induced deaminase (AID) and completed by error-prone processing of the resulting uracils by mismatch and base excision repair factors. Somatic hypermutation represents a threat to genome integrity and it is not known how the B cell genome is protected from the mutagenic effects of somatic hypermutation nor how often these protective mechanisms fail. Here we show, by extensive sequencing of murine B cell genes, that the genome is protected by two distinct mechanisms: selective targeting of AID and gene-specific, high-fidelity repair of AID-generated uracils. Numerous genes linked to B cell tumorigenesis, including Myc, Pim1, Pax5, Ocab (also called Pou2af1), H2afx, Rhoh and Ebf1, are deaminated by AID but escape acquisition of most mutations through the combined action of mismatch and base excision repair. However, approximately 25% of expressed genes analysed were not fully protected by either mechanism and accumulated mutations in germinal centre B cells. Our results demonstrate that AID acts broadly on the genome, with the ultimate distribution of mutations determined by a balance between high-fidelity and error-prone DNA repair.     

Essay review

Benjamin Franklin, the colonial American, maintained a now little-known interest in geological questions for more than sixty years. He began as a follower of English theorists, but soon assimilated some of their ideas with original speculations and discoveries, particularly regarding earthquakes. Though Franklin became famous for his experiments with electricity, he never attempted to explain earthquakes as if they were electrical phenomena; others, however, did. Through his access to American materials, Franklin contributed significantly to the work of several English and French geological theorists. Though some of his own theories were ultimately of limited value, Franklin played an important role in the international science of his time. In addition to his other accomplishments, he was colonial America's foremost student of geology.     

Greek angles from Babylonian numbers

Models of planetary motion as observed from Earth must account for two principal anomalies: the nonuniform speed of the planet as it circles the zodiac, and the correlation of the planet’s position with the position of the Sun. In the context of the geometrical models used by the Greeks, the practical difficulty is to somehow isolate the motion of the epicycle center on the deferent from the motion of the planet on its epicycle. One way to isolate the motion of the epicycle center is to determine the longitude and time of oppositions of the planet with the mean Sun. A Greek astronomer might have realized that the predictions of mean oppositions by Babylonian models could serve as useful proxies for real empirical observations. It is shown that a Greek astronomer with a reasonable understanding of Babylonian System A models for the outer planets and the Sun–Moon could have used those models to estimate approximate values for the eccentricity e and longitude of apogee A required for geometrical models. The same method would work for the inner planets if conjunctions were observable, but they are not, and the variation of the observable synodic events—first and last morning and evening visibilities—is dominated more by the motion of the planet in latitude than the nonuniform motion of the epicycle center.     

The bryozoan genus Conopeum (Electridae) in New Zealand,with description of a new species and discussion of the morphological and genetic characters of Conopeum seurati (Canu, 1928)

ABSTRACT

The status of Conopeum Gray, 1848 in New Zealand is clarified, confirming the presence of three living species – one a naturalised alien, one new to science, and a third that is also known in the Pleistocene. Conopeum seurati (Canu, 1928), a Mediterranean-European species, is naturalised at three localities in New Zealand – Opua, Whangarei Harbour and Whanganui Inlet. Conopeum antipodum n. sp., previously confused with C. seurati, is an estuarine species distributed from Kaipara Harbour to Lyttelton Harbour and is also found in Te Whanga Lagoon at Chatham Island (all New Zealand localities). Conopeum oretiensis Uttley, 1951, first described from Foveaux Strait, is known as far north as Kaipara and Manukau Harbours and is known as a Pleistocene fossil from Napier, New Zealand. There is one other solely fossil New Zealand species – Plio-Pleistocene Electra ongleyi Brown, 1952 is transferred to Conopeum. Sequence data from the 18SrDNA locus confirm that Conopeum antipodum n. sp. is resolved within Conopeum and is distinct from C. seurati.

http://www.zoobank.org/urn:lsid:zoobank.org:act:4FED6730-1C70-4420-B1DA-F1D9046221DF http://www.zoobank.org/urn:lsid:zoobank.org.pub:5C130A99-0869-44A2-885C-338005FBCE07     

老龄健康影响因素的跨学科研究国际动态

本文从四方面综述并讨论老龄健康跨学科研究的国际新动态与进展:(1)发达国家高度重视和不断加强对老龄健康跨学科研究及其研究策略选择;(2)老龄健康的社会行为和环境影响因素研究;(3)老龄健康相关遗传基因研究;(4)社会行为、环境、遗传因素的交互作用对老龄健康影响研究.同时阐述国际上关于老龄健康跨学科研究的若干案例以及我国关于老龄健康影响因素调查和健康长寿候选基因研究方面取得的一些可喜进展与仍然十分薄弱的现状.最后对今后如何开展老龄健康的跨自然与社会科学综合交叉研究提出思考与展望,认为积极推进我国老龄健康跨学科研究势在必行,而我国学者可望为人类应对人口老化严峻挑战做出突出贡献.