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901.
木槿愈伤组织耐盐细胞系是在含NaCl的培养基上通过屡代选择产生的,在无盐培养其中进行几代继代培养后的愈伤组织,由其分化的芽以及脱分化增殖的愈伤组织均表现了耐盐性,木槿耐盐细胞由于对NaCl的反应比未经选择的细胞积累了更多的N^+,K^+,而且细胞体积较小。 相似文献
902.
Spectral analysis of phylogenetic data 总被引:12,自引:0,他引:12
The spectral analysis of sequence and distance data is a new approach to phylogenetic analysis. For two-state character sequences,
the character values at a given site split the set of taxa into two subsets, a bipartition of the taxa set. The vector which
counts the relative numbers of each of these bipartitions over all sites is called a sequence spectrum. Applying a transformation
called a Hadamard conjugation, the sequence spectrum is transformed to the conjugate spectrum. This conjugation corrects for
unobserved changes in the data, independently from the choice of phylogenetic tree. For any given phylogenetic tree with edge
weights (probabilities of state change), we define a corresponding tree spectrum. The selection of a weighted phylogenetic
tree from the given sequence data is made by matching the conjugate spectrum with a tree spectrum. We develop an optimality
selection procedure using a least squares best fit, to find the phylogenetic tree whose tree spectrum most closely matches
the conjugate spectrum. An inferred sequence spectrum can be derived from the selected tree spectrum using the inverse Hadamard
conjugation to allow a comparison with the original sequence spectrum.
A possible adaptation for the analysis of four-state character sequences with unequal frequencies is considered. A corresponding
spectral analysis for distance data is also introduced. These analyses are illustrated with biological examples for both distance
and sequence data. Spectral analysis using the Fast Hadamard transform allows optimal trees to be found for at least 20 taxa
and perhaps for up to 30 taxa.
The development presented here is self contained, although some mathematical proofs available elsewhere have been omitted.
The analysis of sequence data is based on methods reported earlier, but the terminology and the application to distance data
are new. 相似文献
903.
Three polyhydroxylated sterol hemiacetals, pectinoacetals A-C (1–3) have been isolated as their acetyl derivatives (4–6) from the acetic anhydride treated organic extract of the Indo-Pacific gorgonianCtenocella pectinata. These natural products were found to undergo very rapid epimerization at the C-18 chiral center and thus exist only as an equilibrium mixture of two diastereomers. The structure assignments are based on spectral studies and chemical modifications of the natural products. 相似文献
904.
Treatment with the co-transport inhibitor, furosemide decreased36Cl– flux across perfused Malpighian tubules ofLocusta. However, exclusion of36Cl– from the bathing medium had not effect on22Na+ flux whereas substitution of bathing medium Na+ by K+ increased36Cl– flux. Diuretic extract of corpora cardiaca increased22Na+ (by 106%) and36Cl– (by 335%) fluxes differentially. 相似文献
905.
Sexual isolation betweenDrosophila melanogaster females andD. simulans males. Male mating propensities versus success in hybridization 总被引:1,自引:0,他引:1
J. A. Jamart M. C. Carracedo P. Casares 《Cellular and molecular life sciences : CMLS》1993,49(6-7):596-598
Genetic variation has been found in males of aD. simulans population for their eagerness to hybridize withD. melanogaster females. In a search for traits involved in this hybridization, males ofD. simulans were tested for mating speed and sexual vigour. Between-male differences were detected in both sexual traits, but no relationship was noticed between them, nor with the frequency of hybridization. Thus male mating propensities appear to be unrelated to the breakdown of sexual isolation between these sibling species. 相似文献
906.
There are many examples of patterned developing systems which are size invariant: if the total size of the system is reduced, then the pattern responds by changing its scale in such a way that the number of pattern elements remains constant. This phenomenon is one of the bases which underaly the formulation of the concept of positional information, one of the great unifying ideas in developmental biology. However, there are less common examples of patterns which are size dependent. In these, alterations of overall size lead to a reduction in pattern elements. Such size-dependent patterns are therefore of theoretical interest. Here we describe how the number of feather germs along the wing bud of the developing chick embryo responds to shortening of the limb, and consider the implications of these observations. 相似文献
907.
Flavins generate oxidants during metabolism and when exposed to light. Here we report that the photoreceptor layer of retinas from black-eyed rats is reduced in size by a dietary regime containing excess riboflavin. The effect of excess riboflavin was dose-dependent and was manifested by a decrease in photoreceptor length. This decrease was due in part to a reduction in the thickness of the outer nuclear layer, a structure formed from stacked photoreceptor nuclei. These changes were accompanied by an increase in photoreceptor outer segment autofluorescence following illumination at 328 nm, a wavelength that corresponds to the excitation maxima of oxidized lipopigments of the retinal pigment epithelium. 相似文献
908.
R. J. Capon K. Elsbury M. S. Butler C. C. Lu J. N. A. Hooper J. A. P. Rostas K. J. O'Brien L. -M. Mudge A. T. R. Sim 《Cellular and molecular life sciences : CMLS》1993,49(3):263-264
The Antarctic marine spongeTedania charcoti has been shown to contain extraordinarily high natural concentrations of cadmium and zinc, which have in turn been correlated to the ability of the crude ethanol extract to modulate protein phosphorylation in chicken forebrain and to inhibit the growth of several test bacteria. 相似文献
909.
C. Brugnara 《Cellular and molecular life sciences : CMLS》1993,49(2):100-109
The cellular concentration of Hb S plays a central role in the kinetic of Hb S polymerization and cell sickling. Blood of patients with homozygous sickle cell (SS) anemia contains a variable fraction of cells which are markedly dehydrated and have increased Hb S concentration. Since a decrease in cellular Hb S concentration reduces Hb S polymerization and sickling, the study of the processes leading to sickle cell dehydration has important pathophysiological and therapeutic implications. Sickle cell dehydration is due to cellular loss of K and Cl. K loss in sickle cells can take place via either the Ca2+-activated K+ channel, or the K?Cl cotransport, or the combined effect of oxidative damage and deformation of the red cell membrane. Inhibitors of K transport through these pathways could be used to prevent dehydration of sickle cells in vivo, provided that they can be administered safely. 相似文献
910.
Sex in Caenorhabditis elegans is determined by a regulatory cascade of seven interacting autosomal genes controlled by three X-linked genes in response to the X chromosome-to-autosome (X/A) ratio. XX animals (high X/A) develop as self-fertile hermaphrodites, and XO animals (low X/A) develop as males. The activity of the first gene in the sex-determining cascade, her-1, is required for male sexual development. XO her-1 loss-of-function mutants develop as self-fertile hermaphrodites, whereas XX her-1 gain-of-function mutants develop as masculinized intersexes. By genetic mosaic analysis using a fused free duplication linking her-1 to a cell-autonomous marker gene, we show here that her-1 expression in a sexually dimorphic cell is neither necessary nor sufficient for that cell to adopt a male fate. Our results suggest that her-1 is expressed in many, possibly all, cells and that its gene product can function non-autonomously through cell interactions to determine male sexual development. 相似文献