Rebasing the annual mid-year population estimates for England and Wales

The ONS produces mid-year population estimates annually, which are based on updating from the most recent census. Therefore, whenever results become available from a census, a new base is created for the population estimates. This has implications for historic series, which need to be revised to be consistent with both the past and the most recent census. This article describes the methodology that will be used for this rebasing of the mid-year population estimates following the availability of results from the 2001 Census. Census results also provide a unique opportunity to assess the accuracy of the population estimates that are based on the previous census and this article also describes the approach that will be taken to the assessment of accuracy.     

Contemporary fisherian life-history evolution in small salmonid populations

The relative importance of natural selection and random drift in phenotypic evolution has been discussed since the introduction of the first population genetic models. The empirical evidence used to evaluate the evolutionary theories of Fisher and Wright remains obscure because formal tests for neutral divergence or sensitive attempts to separate the effects of selection and drift are scarce, subject to error, and have not been interpreted in the light of well-known population demography. We combined quantitative genetic and microsatellite DNA analyses to investigate the determinants of contemporary life-history evolution in isolated populations of grayling (Thymallus thymallus, Salmonidae) that originated from a common source 80-120 years ago. Here we show that natural selection was the dominant diversifying agent in the evolution of the quantitative traits. However, the populations were founded by a small number of individuals, exhibit very low microsatellite-based effective sizes and show genetic imprints of severe 'bottlenecks'; which are conditions often suggested to constrain selection and favour drift. This study demonstrates a very clear case of fisherian evolution in small natural populations across a contemporary timescale.     

Plasmodium, human and Anopheles genomics and malaria

The Plasmodium spp. parasites that cause malaria are transmitted to humans by Anopheles spp. mosquitoes. Scientists have now amassed a great body of knowledge about the parasite, its mosquito vector and human host. Yet this year there will be 300-500 million new malaria infections and 1-3 million deaths caused by the disease. We believe that integrated analyses of genome sequence, DNA polymorphisms, and messenger RNA and protein expression profiles will lead to greater understanding of the molecular basis of vector-human and host-parasite interactions and provide strategies to build upon these insights to develop interventions to mitigate human morbidity and mortality from malaria.     

Some relationships of black-tailed prairie dogs to livestock grazing

Reproductive ecology of black-tailed prairie dogs ( Cynomys ludovicianus ) was studied on the Charles M. Russell National Wildlife Refuge in northeastern Montana (1978–1980 and 1985). Breeding took place from early March through early April in most years. Persistent snow cover and below normal temperatures in February and March of 1978 delayed the start of breeding. Litter size averaged 4.4 but varied significantly among years. Average yearly litter size was correlated (r 2 = 0.986) with summer (June-September) precipitation prior to the breeding season. Largest average yearly litter size (5.0) followed record precipitation, while the smallest average yearly litter size (3.8) followed extreme drought. More than half the yearling females failed to breed while 88% of the females two years and older bred. Testes weights were greatest early in the breeding season and regressed rapidly during April.     

Managing a complex exotic vegetation program in Yellowstone National Park

The number of documented exotic plants in Yellowstone National Park has increased from 85 known in 1986 to over 185 today. Exotic plants are substantially impacting the parks natural and cultural resources and are a high management priority. We have adopted an integrated weed management approach with regard to exotic vegetation, emphasizing prevention, education, early detection and eradication, control, and, to a lesser degree, monitoring. The program involves over 140 staff with program expenditures averaging approximately $190,000 annually. Prevention actions include requiring approved gravel on construction projects; banning hay in the backcountry and allowing transport of only certified weed-seed-free hay through Yellowstone; requiring construction equipment to be pressurecleaned prior to entering the park; and native species revegetation after road, housing, and other construction projects have disturbed ground. Over 4500 acres, primarily along roadsides and in developed areas, are surveyed annually in early detection efforts with emphasis placed on eradicating small, new infestations of highly invasive species such as sulfur cinquefoil ( Potentilla recta L.) and leafy spurge ( Euphorbia esula L.). Control efforts focus on about 30 priority species, such as spotted knapweed ( Centaurea maculosa Lam.), oxeye daisy ( Chrysanthemum leucanthemum L.), and hoary cress ( Cardaria draba [L.] Desv.) using chemical, mechanical, and cultural techniques. A total of 2027 acres were treated during 1998, whereas control efforts for 12 species occurred on 2596 acres during the previous 3-year period, 1995-1997. Strong and expanding partnerships with other federal, state, and local agencies and private companies contribute to management efforts within the park. Future program goals emphasize increases in base funding to ensure continued weed management efforts as well as expanding survey, monitoring, and reclamation efforts. Ultimately, a more rigorous assessment of program effectiveness is desired.     

Nicotinic acetylcholine receptors in attention circuitry: the role of layer VI neurons of prefrontal cortex

Cholinergic modulation of prefrontal cortex is essential for attention. In essence, it focuses the mind on relevant, transient stimuli in support of goal-directed behavior. The excitation of prefrontal layer VI neurons through nicotinic acetylcholine receptors optimizes local and top-down control of attention. Layer VI of prefrontal cortex is the origin of a dense feedback projection to the thalamus and is one of only a handful of brain regions that express the α5 nicotinic receptor subunit, encoded by the gene chrna5. This accessory nicotinic receptor subunit alters the properties of high-affinity nicotinic receptors in layer VI pyramidal neurons in both development and adulthood. Studies investigating the consequences of genetic deletion of α5, as well as other disruptions to nicotinic receptors, find attention deficits together with altered cholinergic excitation of layer VI neurons and aberrant neuronal morphology. Nicotinic receptors in prefrontal layer VI neurons play an essential role in focusing attention under challenging circumstances. In this regard, they do not act in isolation, but rather in concert with cholinergic receptors in other parts of prefrontal circuitry. This review urges an intensification of focus on the cellular mechanisms and plasticity of prefrontal attention circuitry. Disruptions in attention are one of the greatest contributing factors to disease burden in psychiatric and neurological disorders, and enhancing attention may require different approaches in the normal and disordered prefrontal cortex.     

Initial genome sequencing and analysis of multiple myeloma

Multiple myeloma is an incurable malignancy of plasma cells, and its pathogenesis is poorly understood. Here we report the massively parallel sequencing of 38 tumour genomes and their comparison to matched normal DNAs. Several new and unexpected oncogenic mechanisms were suggested by the pattern of somatic mutation across the data set. These include the mutation of genes involved in protein translation (seen in nearly half of the patients), genes involved in histone methylation, and genes involved in blood coagulation. In addition, a broader than anticipated role of NF-κB signalling was indicated by mutations in 11 members of the NF-κB pathway. Of potential immediate clinical relevance, activating mutations of the kinase BRAF were observed in 4% of patients, suggesting the evaluation of BRAF inhibitors in multiple myeloma clinical trials. These results indicate that cancer genome sequencing of large collections of samples will yield new insights into cancer not anticipated by existing knowledge.