全文获取类型
收费全文 | 124篇 |
免费 | 0篇 |
国内免费 | 1篇 |
专业分类
系统科学 | 3篇 |
理论与方法论 | 1篇 |
现状及发展 | 31篇 |
研究方法 | 27篇 |
综合类 | 59篇 |
自然研究 | 4篇 |
出版年
2021年 | 1篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2015年 | 2篇 |
2014年 | 4篇 |
2013年 | 6篇 |
2012年 | 16篇 |
2011年 | 19篇 |
2010年 | 5篇 |
2009年 | 1篇 |
2008年 | 11篇 |
2007年 | 12篇 |
2006年 | 6篇 |
2005年 | 10篇 |
2004年 | 3篇 |
2003年 | 5篇 |
2002年 | 7篇 |
1988年 | 2篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1971年 | 1篇 |
1963年 | 1篇 |
1961年 | 1篇 |
排序方式: 共有125条查询结果,搜索用时 15 毫秒
121.
The mammalian olfactory system detects and discriminates thousands of odorants using many different receptors expressed by sensory neurons in the nasal epithelium. Axonal projections from these neurons to the main olfactory bulbs form reproducible patterns of glomeruli in two widely separated regions of each bulb, creating two mirror-symmetric maps of odorant receptor projections. To investigate whether odorant receptors organize neural circuitry in the olfactory bulb, we have examined a genetically modified mouse line, rI7 --> M71, in which a functionally characterized receptor, rI7, has been substituted into the M71 receptor locus. Here we show that despite their ectopic location the resulting glomeruli are responsive to known ligands of the rI7 receptor, attract postsynaptic innervation by mitral/tufted cell dendrites, and endow these cells with responses that are characteristic of the rI7 receptor. External tufted cells receiving input from rI7 --> M71 glomeruli form precise intrabulbar projections that link medial and lateral rI7 --> M71 glomeruli anatomically, thus providing a substrate for coordinating isofunctional glomeruli. We conclude that odorant receptor identity in epithelial neurons determines not only glomerular convergence and function, but also functional circuitry in the olfactory bulb. 相似文献
122.
Ptprj is a candidate for the mouse colon-cancer susceptibility locus Scc1 and is frequently deleted in human cancers 总被引:21,自引:0,他引:21
123.
A lentivirus-based system to functionally silence genes in primary mammalian cells,stem cells and transgenic mice by RNA interference 总被引:87,自引:0,他引:87
124.
Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation 总被引:1,自引:0,他引:1
Rocap G Larimer FW Lamerdin J Malfatti S Chain P Ahlgren NA Arellano A Coleman M Hauser L Hess WR Johnson ZI Land M Lindell D Post AF Regala W Shah M Shaw SL Steglich C Sullivan MB Ting CS Tolonen A Webb EA Zinser ER Chisholm SW 《Nature》2003,424(6952):1042-1047
The marine unicellular cyanobacterium Prochlorococcus is the smallest-known oxygen-evolving autotroph. It numerically dominates the phytoplankton in the tropical and subtropical oceans, and is responsible for a significant fraction of global photosynthesis. Here we compare the genomes of two Prochlorococcus strains that span the largest evolutionary distance within the Prochlorococcus lineage and that have different minimum, maximum and optimal light intensities for growth. The high-light-adapted ecotype has the smallest genome (1,657,990 base pairs, 1,716 genes) of any known oxygenic phototroph, whereas the genome of its low-light-adapted counterpart is significantly larger, at 2,410,873 base pairs (2,275 genes). The comparative architectures of these two strains reveal dynamic genomes that are constantly changing in response to myriad selection pressures. Although the two strains have 1,350 genes in common, a significant number are not shared, and these have been differentially retained from the common ancestor, or acquired through duplication or lateral transfer. Some of these genes have obvious roles in determining the relative fitness of the ecotypes in response to key environmental variables, and hence in regulating their distribution and abundance in the oceans. 相似文献
125.
Römer W Berland L Chambon V Gaus K Windschiegl B Tenza D Aly MR Fraisier V Florent JC Perrais D Lamaze C Raposo G Steinem C Sens P Bassereau P Johannes L 《Nature》2007,450(7170):670-675
Clathrin seems to be dispensable for some endocytic processes and, in several instances, no cytosolic coat protein complexes could be detected at sites of membrane invagination. Hence, new principles must in these cases be invoked to account for the mechanical force driving membrane shape changes. Here we show that the Gb3 (glycolipid)-binding B-subunit of bacterial Shiga toxin induces narrow tubular membrane invaginations in human and mouse cells and model membranes. In cells, tubule occurrence increases on energy depletion and inhibition of dynamin or actin functions. Our data thus demonstrate that active cellular processes are needed for tubule scission rather than tubule formation. We conclude that the B-subunit induces lipid reorganization that favours negative membrane curvature, which drives the formation of inward membrane tubules. Our findings support a model in which the lateral growth of B-subunit-Gb3 microdomains is limited by the invagination process, which itself is regulated by membrane tension. The physical principles underlying this basic cargo-induced membrane uptake may also be relevant to other internalization processes, creating a rationale for conceptualizing the perplexing diversity of endocytic routes. 相似文献