Cloning, construction of prokaryotic expression vector and expression ofEscherichia coli cytosine deaminase gene

Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank.     

极性晶体膜中极化子的性质

使用格林函数法研究在极性晶体膜中极化子自能的一些性质．对ＧａＡｓ进行了计算,结果表明：在绝对零度时,极性晶体膜中电子与体纵光学声子相互作用能的绝对值随极性晶体膜的厚度的增加而增加,随后几乎保持不变．     

海浪统计资料对船舶波浪弯矩设计值的影响

对船舶特别是超规范船舶进行结构的直接计算设计时,设计人员应合理选择船舶航行海域的海浪长期统计资料,根据船舶的装载工况及实际可能达到的航速,借用线性理论或非线性理论进行波浪弯矩的长期预报来确定设计值．文中就一艘大型集装箱船舶,按五个典型海域的海浪统计资料,对波浪弯矩及波浪合成弯矩作了长期预报计算,并与ＩＡＣＳ的统一纵强度要求ＵＲ－Ｓ１１的结果作比较和分析,给出了对船舶结构直接计算设计有指导意义的几点结论．     

金电极的逐层自组装修饰

本文运用自组装技术用ω-巯基己酸化学修饰金电极形成第一层修饰层,ω-巯基己酸暴露在外表面的羧基活性基团还可在气相条件下进行酰胺化反应键接上乙二胺,从而实现逐层自组装修饰金电极.同时对此修饰电极也进行了电化学表征.     

一种基于H∞技术的新型鲁棒励磁控制器

论述了微机励磁控制器硬件电路设计及控制规律,微机励磁控制器硬件采用模块化设计,四种控制规律：比例式、比例式＾＋电力系统稳定器、线性最优控制、基于Ｈ∞技术鲁棒性控制。通过对单机无穷在系统小干扰、大干扰仿真表明,线性最优控制和基于Ｈ∞技术鲁棒控制较前两种控制规律为优,它们不仅能有效的提高系统的静态性能,而且在大干扰情况下,也能够为系统提供阻尼,改善系统的动态品质。     

前驱体溶液pH值对溶胶凝胶制备钛酸铋薄膜的影响

采用溶胶凝胶技术在石英玻璃基片上制备钛酸铋薄膜,讨论了前驱体溶液的酸碱度（ｐＨ值）对薄膜结构和结晶性的影响．ｐＨ＜２和ｐＨ＞３５的前驱体溶液所制备的薄膜在晶化过程中出现Ｂｉ２Ｔｉ２Ｏ７和Ｂｉ２Ｔｉ４Ｏ１１杂相．ｐＨ＝２５～３５的前驱体溶液制备的薄膜是钙钛矿Ｂｉ４Ｔｉ３Ｏ１２结构的取向薄膜     

Bi4Ti3O12铁电薄膜溶胶—凝胶法制备过程中Bi—Ti溶胶的匀胶规律研究

介绍了Ｓｏｌｇｅｌ法制备Ｂｉ４Ｔｉ３Ｏ１２薄膜的工艺过程,研究了ＢｉＴｉ溶胶在Ｓｉ基片上的匀胶规律;用ＴＧＤＴＡ和ＴＥＭ技术研究了ＢｉＴｉ干凝胶的形态,并成功地在Ｐｔ／Ｔｉ／Ｓｉ基片上制备了ｃ轴取向的Ｂｉ４Ｔｉ３Ｏ１２薄膜．     

Dissection of the pheromone-modulated flight of moths using single-pulse response as a template

The upwind flight of male moths to conspecific females is mediated by the chemical and structural characteristics of a pheromone plume. We describe the reaction of maleCadra cautella, the almond moth, to the interception of single pulses of sex pheromone, the smallest structural units of odour plumes. Following loss of a pheromone plume, males cast, that is fly a crosswind course without progressing upwind. The response of casting males to interception of a pulse of 0.25 s duration was, after a delay of 0.21±0.07 s, to turn and briefly fly straighter upwind, resulting in average net upwind displacements of 18 cm in a 50 cm s^–1 wind. Upwind progress in the single-pulse response was the result of steering more upwind and an increase in airspeed, although average ground speed remained unchanged. During the last third of the surge, males turned crosswind, returning to casting flight. These behavioural reactions to pheromone contact and loss support the phasic-tonic model of odour-modulated flight, in which an underlying tonic counterturning rhythm, expressed upon pheromone loss, is briefly overridden by phasic upwind surges, expressed upon interception of the pheromone filament. The surge portion of the cast-surge-cast response was diminished and more crosswind if individual pulses were shorter (0.02 s), probably due to sub-optimal contact with pheromone. The cast-surge-cast response to interception of a single 0.25 s pulse was used as a template to interpret the form of flight tracks in plumes of known structure. The template matched portions of flight tracks of males flying in plumes of low pheromone pulse frequency, thus reflecting the male's pattern of pulse encounter. In plumes ensuring a high frequency of pulse interception, only the upwind surge portion of the template was expressed, resulting in nearly straight upwind flight tracks. Similar nearly straight upwind flight tracks occurred in flights along plumes of low pulse frequency with large volume. Thus flight tracks of maleC. cautella to point sources of pheromone depend on both the frequency and the size of filaments encountered.     

苦楝果实活性物质对植物生长调节作用

应用生物活性鉴定技术,ＴＬＣ及化学成分检验法,论证了苦楝果实中含有对植物生长促进的活性性质,并初步证明这些活性物质为极性较大的萜类化合物,笔者将这些活性物质为“苦楝生长素类”。     

Transplantation of human cortex with Alzheimer's disease into rat occipital cortex; a model for the study of Alzheimer disease

Summary Senile dementia of the Alzheimer type (SDAT) is a major problem in the human senescent population. As this pathology cannot be reproduced in animals, research into its development is greatly impeded. The technique of implantation of the nervous tissue has been utilized in order to establish an animal model and to test the possible existence of a transmissible agent. When human temporal cortex with Alzheimer's disease is implanted in the occipital cortex of 7-week-old rats, human cerebral tissue containing abundant tangles induces in the receiver cortex a reactive fibrous gliosis. In the processes of the astrocytes, twisted filaments are evident among bundles of normal filaments. These alterations could be induced by the metabolising of abnormal filament subunits or by some infectious agent introduced by the implant.This study is supported by grant No. 2.4517.82 of Fonds de la Recherche Fondamentale Collective of Belgium.