哈密顿振幅方程的新的精确解

利用双曲函数法求出了一个新的哈密顿振幅方程ux＋utt＋2σ│u│^2u-εuxt=0的新的精确解。     

微细硅微粉的混酸法纯化条件研究

研究了盐酸一硫酸法对粒度为1-5μm的四川硅微粉的纯化方法.最佳工艺条件为盐酸浓度20％;液固比:盐酸 溶液:硅微粉为1.5:1:硫酸浓度25％:液固比:硫酸:硅微粉为2:1;硫酸浸溶时间为12h.纯化处理后硅微粉中铁 的含量<60μg·g-1.     

文学翻译之"同词异境异意"刍议

翻译过程中的文化差异,两种语言的文体、语体、语境等诸多因素的不同与制约,必然会造成内容、形式、意义等各方面的翻译流失。文章结合当前语言学中的最新前沿学科语用学的相关知识,按照翻译的一般认知规律与过程,初探文学翻译中“同词异境异意”现象,从翻译的语境认知转向探讨文学翻译。     

汞离子胁迫对茭白及其体内黑粉菌的影响

研究了Hg^2＋对茭白（Zizania latifolia Turcz．）体内共生的茭白黑粉菌（Ustilago dctdenta P．Henn．）的影响．对不同浓度Hg^2＋胁迫下茭白黑粉菌在幼茎中的分布状态进行了解剖观察，分析了在此过程中的MDA含量、可溶性蛋白含量、SOD活性和POD活性等一系列指标．试验结果表明，2mg／L以上的Hg^2＋胁迫能导致茭白黑粉菌的消亡，导致茭白茎尖不能膨大．     

油气分离器内油滴运动轨迹的数值模拟

在不考虑相间相互作用的条件下，气相采用RNG k-ε（重整化群）湍流模型，油滴相采用随机轨道模型，对油气分离器一次油分内的油气两相流动进行了数值模拟，揭示了油气分离器内的流动分布和油滴的运动轨迹及分离机理．计算结果表明：一次油分内的速度场分布不均匀，而且靠近挡板底面附近的气流速度很小，这样易使进入二次油分滤芯前较小的油滴分离；另外，不同粒径的油滴其运动轨迹差别很大，很明显，粒径大的油滴较容易分离；油滴入射的初始位置不同，其分离速度的差别较大，运动轨迹也明显不同．     

水泥石中羟钙石的X射线衍射定量分析

用x衍射法对水泥石中羟钙石作了定量分析,得到了比较准确的结果,这些实验结果对阐述羟钙石的形成机理和抗腐蚀机理有理论和实际意义。     

Multiple flavonoid-binding sites within multidrug resistance protein MRP1

Recombinant nucleotide-binding domains (NBDs) from human multidrug resistance protein MRP1 were overexpressed in bacteria and purified to measure their direct interaction with high-affinity flavonoids, and to evaluate a potential correlation with inhibition of MRP1-mediated transport activity and reversion of cellular multidrug resistance. Among different classes of flavonoids, dehydrosilybin exhibited the highest affinity for both NBDs, the binding to N-terminal NBD1 being prevented by ATP. Dehydrosilybin increased vanadate-induced 8-N₃-[-³²P]ADP trapping, indicating stimulation of ATPase activity. In contrast, dehydrosilybin strongly inhibited leukotriene C₄ (LTC₄) transport by membrane vesicles from MRP1-transfected cells, independently of reduced glutathione, and chemosensitized cell growth to vincristine. Hydrophobic C-isoprenylation of dehydrosilybin increased the binding affinity for NBD1, but outsite the ATP site, lowered the increase in vanadate-induced 8-N₃-[-³²P]ADP trapping, weakened inhibition of LTC₄ transport which became glutathione dependent, and induced some cross-resistance. The overall results indicate multiple binding sites for dehydrosilybin and its derivatives, on both cytosolic and transmembrane domains of MRP1.Received 1 May 2003; received after revision 18 June 2003; accepted 24 June 2003     

Trans allele methylation and paramutation-like effects in mice

In mammals, imprinted genes have parent-of-origin-specific patterns of DNA methylation that cause allele-specific expression. At Rasgrf1 (encoding RAS protein-specific guanine nucleotide-releasing factor 1), a repeated DNA element is needed to establish methylation and expression of the active paternal allele. At Igf2r (encoding insulin-like growth factor 2 receptor), a sequence called region 2 is needed for methylation of the active maternal allele. Here we show that replacing the Rasgrf1 repeats on the paternal allele with region 2 allows both methylation and expression of the paternal copy of Rasgrf1, indicating that sequences that control methylation can function ectopically. Paternal transmission of the mutated allele also induced methylation and expression in trans of the normally unmethylated and silent wild-type maternal allele. Once activated, the wild-type maternal Rasgrf1 allele maintained its activated state in the next generation independently of the paternal allele. These results recapitulate in mice several features in common with paramutation described in plants.     

Different domains of synaptotagmin control the choice between kiss-and-run and full fusion

Exocytosis-the release of the contents of a vesicle--proceeds by two mechanisms. Full fusion occurs when the vesicle and plasma membranes merge. Alternatively, in what is termed kiss-and-run, vesicles can release transmitter during transient contacts with the plasma membrane. Little is known at the molecular level about how the choice between these two pathways is regulated. Here we report amperometric recordings of catecholamine efflux through individual fusion pores. Transfection with synaptotagmin (Syt) IV increased the frequency and duration of kiss-and-run events, but left their amplitude unchanged. Endogenous Syt IV, induced by forskolin treatment, had a similar effect. Full fusion was inhibited by mutation of a Ca2+ ligand in the C2A domain of Syt I; kiss-and-run was inhibited by mutation of a homologous Ca2+ ligand in the C2B domain of Syt IV. The Ca2+ sensitivity for full fusion was 5-fold higher with Syt I than Syt IV, but for kiss-and-run the Ca2+ sensitivities differed by a factor of only two. Syt thus regulates the choice between full fusion and kiss-and-run, with Ca2+ binding to the C2A and C2B domains playing an important role in this choice.