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51.
Metzger T Gache V Xu M Cadot B Folker ES Richardson BE Gomes ER Baylies MK 《Nature》2012,484(7392):120-124
The basic unit of skeletal muscle in all metazoans is the multinucleate myofibre, within which individual nuclei are regularly positioned. The molecular machinery responsible for myonuclear positioning is not known. Improperly positioned nuclei are a hallmark of numerous diseases of muscle, including centronuclear myopathies, but it is unclear whether correct nuclear positioning is necessary for muscle function. Here we identify the microtubule-associated protein ensconsin (Ens)/microtubule-associated protein 7 (MAP7) and kinesin heavy chain (Khc)/Kif5b as essential, evolutionarily conserved regulators of myonuclear positioning in Drosophila and cultured mammalian myotubes. We find that these proteins interact physically and that expression of the Kif5b motor domain fused to the MAP7 microtubule-binding domain rescues nuclear positioning defects in MAP7-depleted cells. This suggests that MAP7 links Kif5b to the microtubule cytoskeleton to promote nuclear positioning. Finally, we show that myonuclear positioning is physiologically important. Drosophila ens mutant larvae have decreased locomotion and incorrect myonuclear positioning, and these phenotypes are rescued by muscle-specific expression of Ens. We conclude that improper nuclear positioning contributes to muscle dysfunction in a cell-autonomous fashion. 相似文献
52.
Read KA Mahajan AS Carpenter LJ Evans MJ Faria BV Heard DE Hopkins JR Lee JD Moller SJ Lewis AC Mendes L McQuaid JB Oetjen H Saiz-Lopez A Pilling MJ Plane JM 《Nature》2008,453(7199):1232-1235
Increasing tropospheric ozone levels over the past 150 years have led to a significant climate perturbation; the prediction of future trends in tropospheric ozone will require a full understanding of both its precursor emissions and its destruction processes. A large proportion of tropospheric ozone loss occurs in the tropical marine boundary layer and is thought to be driven primarily by high ozone photolysis rates in the presence of high concentrations of water vapour. A further reduction in the tropospheric ozone burden through bromine and iodine emitted from open-ocean marine sources has been postulated by numerical models, but thus far has not been verified by observations. Here we report eight months of spectroscopic measurements at the Cape Verde Observatory indicative of the ubiquitous daytime presence of bromine monoxide and iodine monoxide in the tropical marine boundary layer. A year-round data set of co-located in situ surface trace gas measurements made in conjunction with low-level aircraft observations shows that the mean daily observed ozone loss is approximately 50 per cent greater than that simulated by a global chemistry model using a classical photochemistry scheme that excludes halogen chemistry. We perform box model calculations that indicate that the observed halogen concentrations induce the extra ozone loss required for the models to match observations. Our results show that halogen chemistry has a significant and extensive influence on photochemical ozone loss in the tropical Atlantic Ocean boundary layer. The omission of halogen sources and their chemistry in atmospheric models may lead to significant errors in calculations of global ozone budgets, tropospheric oxidizing capacity and methane oxidation rates, both historically and in the future. 相似文献
53.
54.
Soon after the crowd streamed on to London's Millennium Bridge on the day it opened, the bridge started to sway from side to side: many pedestrians fell spontaneously into step with the bridge's vibrations, inadvertently amplifying them. Here we model this unexpected and now notorious phenomenon--which was not due to the bridge's innovative design as was first thought--by adapting ideas originally developed to describe the collective synchronization of biological oscillators such as neurons and fireflies. Our approach should help engineers to estimate the damping needed to stabilize other exceptionally crowded footbridges against synchronous lateral excitation by pedestrians. 相似文献
55.
为选择更适用的太阳辐射计算模型进行桥梁截面积温度场分析,对国内外不同的太阳辐射计算模型进行归纳总结,主要包括桥梁截面温度场分析中必备的太阳总辐射、太阳直接辐射和太阳散射辐射.对比分析不同数值计算模型之间的异同点及其适用范围.分析发现基于日照时数的日太阳总辐射经验模型ngstr9m-Page模型应用最多,其线性表达式的计算量小且满足精度要求;日太阳总辐射可采用Collares-Pereira and Rabl模型换算为逐时太阳总辐射;逐时太阳直接辐射和逐时太阳散射辐射可分别采用Hottel模型和Liu and Jordan模型进行计算.所总结的逐时太阳辐射计算模型可作为边界条件开展桥梁截面温度场分析. 相似文献
56.
实地采集福建省8 座城市的太阳辐射数据,基于此提出一种适用的数值计算模型. 选取一座实桥,实地监测其主梁截面温度场数据,并采用MIDAS-FEA软件建立有限元模型进行模拟. 有限元模型计算值与实测值对比表明,夏季考虑太阳辐射的截面温度场计算曲线与实测曲线吻合较好;而不考虑太阳辐射的截面温度场计算曲线的峰值低于实测值,且其峰值出现的时间迟于实际值. 冬季太阳辐射对于桥梁截面温度场影响可以忽略不计. 相似文献
57.
Generally, the motion of fluids is smooth and laminar at low speeds but becomes highly disordered and turbulent as the velocity increases. The transition from laminar to turbulent flow can involve a sequence of instabilities in which the system realizes progressively more complicated states, or it can occur suddenly. Once the transition has taken place, it is generally assumed that, under steady conditions, the turbulent state will persist indefinitely. The flow of a fluid down a straight pipe provides a ubiquitous example of a shear flow undergoing a sudden transition from laminar to turbulent motion. Extensive calculations and experimental studies have shown that, at relatively low flow rates, turbulence in pipes is transient, and is characterized by an exponential distribution of lifetimes. They also suggest that for Reynolds numbers exceeding a critical value the lifetime diverges (that is, becomes infinitely large), marking a change from transient to persistent turbulence. Here we present experimental data and numerical calculations covering more than two decades of lifetimes, showing that the lifetime does not in fact diverge but rather increases exponentially with the Reynolds number. This implies that turbulence in pipes is only a transient event (contrary to the commonly accepted view), and that the turbulent and laminar states remain dynamically connected, suggesting avenues for turbulence control. 相似文献
58.
Sayrin C Dotsenko I Zhou X Peaudecerf B Rybarczyk T Gleyzes S Rouchon P Mirrahimi M Amini H Brune M Raimond JM Haroche S 《Nature》2011,477(7362):73-77
Feedback loops are central to most classical control procedures. A controller compares the signal measured by a sensor (system output) with the target value or set-point. It then adjusts an actuator (system input) to stabilize the signal around the target value. Generalizing this scheme to stabilize a micro-system's quantum state relies on quantum feedback, which must overcome a fundamental difficulty: the sensor measurements cause a random back-action on the system. An optimal compromise uses weak measurements, providing partial information with minimal perturbation. The controller should include the effect of this perturbation in the computation of the actuator's operation, which brings the incrementally perturbed state closer to the target. Although some aspects of this scenario have been experimentally demonstrated for the control of quantum or classical micro-system variables, continuous feedback loop operations that permanently stabilize quantum systems around a target state have not yet been realized. Here we have implemented such a real-time stabilizing quantum feedback scheme following a method inspired by ref. 13. It prepares on demand photon number states (Fock states) of a microwave field in a superconducting cavity, and subsequently reverses the effects of decoherence-induced field quantum jumps. The sensor is a beam of atoms crossing the cavity, which repeatedly performs weak quantum non-demolition measurements of the photon number. The controller is implemented in a real-time computer commanding the actuator, which injects adjusted small classical fields into the cavity between measurements. The microwave field is a quantum oscillator usable as a quantum memory or as a quantum bus swapping information between atoms. Our experiment demonstrates that active control can generate non-classical states of this oscillator and combat their decoherence, and is a significant step towards the implementation of complex quantum information operations. 相似文献
59.
Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota 总被引:8,自引:0,他引:8
D Bulgarelli M Rott K Schlaeppi E Ver Loren van Themaat N Ahmadinejad F Assenza P Rauf B Huettel R Reinhardt E Schmelzer J Peplies FO Gloeckner R Amann T Eickhorst P Schulze-Lefert 《Nature》2012,488(7409):91-95
The plant root defines the interface between a multicellular eukaryote and soil, one of the richest microbial ecosystems on Earth. Notably, soil bacteria are able to multiply inside roots as benign endophytes and modulate plant growth and development, with implications ranging from enhanced crop productivity to phytoremediation. Endophytic colonization represents an apparent paradox of plant innate immunity because plant cells can detect an array of microbe-associated molecular patterns (also known as MAMPs) to initiate immune responses to terminate microbial multiplication. Several studies attempted to describe the structure of bacterial root endophytes; however, different sampling protocols and low-resolution profiling methods make it difficult to infer general principles. Here we describe methodology to characterize and compare soil- and root-inhabiting bacterial communities, which reveals not only a function for metabolically active plant cells but also for inert cell-wall features in the selection of soil bacteria for host colonization. We show that the roots of Arabidopsis thaliana, grown in different natural soils under controlled environmental conditions, are preferentially colonized by Proteobacteria, Bacteroidetes and Actinobacteria, and each bacterial phylum is represented by a dominating class or family. Soil type defines the composition of root-inhabiting bacterial communities and host genotype determines their ribotype profiles to a limited extent. The identification of soil-type-specific members within the root-inhabiting assemblies supports our conclusion that these represent soil-derived root endophytes. Surprisingly, plant cell-wall features of other tested plant species seem to provide a sufficient cue for the assembly of approximately 40% of the Arabidopsis bacterial root-inhabiting microbiota, with a bias for Betaproteobacteria. Thus, this root sub-community may not be Arabidopsis-specific but saprophytic bacteria that would naturally be found on any plant root or plant debris in the tested soils. By contrast, colonization of Arabidopsis roots by members of the Actinobacteria depends on other cues from metabolically active host cells. 相似文献
60.
G. Tolone L. Bonasera R. S. Bruno 《Cellular and molecular life sciences : CMLS》1971,27(12):1472-1473
Résumé Étude comparative des proprietés biologiques des anticorps IgM, IgG1 et IgG2 du rat. Les résultats montrent que le pouvoir agglutinant et lytique des anticorps IgM est respectivement 37 et 100 fois supérieur à celui des deux classes d'anticorps IgG. Par contre, en ce qui concerne le phénomène d'Arthus, les anticorps IgM sont moins actifs que les anticorps IgG, si les rapports sont exprimés en poids. Si l'on calcule les rapports par nombre de molécules, les anticorps IgM sont aussi, dans ce cas, plus actifs que les IgG. 相似文献