Aspartate 112 is the selectivity filter of the human voltage-gated proton channel

The ion selectivity of pumps and channels is central to their ability to perform a multitude of functions. Here we investigate the mechanism of the extraordinary selectivity of the human voltage-gated proton channel, H(V)1 (also known as HVCN1). This selectivity is essential to its ability to regulate reactive oxygen species production by leukocytes, histamine secretion by basophils, sperm capacitation, and airway pH. The most selective ion channel known, H(V)1 shows no detectable permeability to other ions. Opposing classes of selectivity mechanisms postulate that (1) a titratable amino acid residue in the permeation pathway imparts proton selectivity, or (2) water molecules 'frozen' in a narrow pore conduct protons while excluding other ions. Here we identify aspartate 112 as a crucial component of the selectivity filter of H(V)1. When a neutral amino acid replaced Asp?112, the mutant channel lost proton specificity and became anion-selective or did not conduct. Only the glutamate mutant remained proton-specific. Mutation of the nearby Asp?185 did not impair proton selectivity, indicating that Asp?112 has a unique role. Although histidine shuttles protons in other proteins, when histidine or lysine replaced Asp?112, the mutant channel was still anion-permeable. Evidently, the proton specificity of H(V)1 requires an acidic group at the selectivity filter.     

Benthos recovery after an episodic sediment release into a Colorado Rocky Mountain river

During late September 1996, approximately 7000 m 3 of clay- to gravel-sized sediment was flushed from Halligan Reservoir, Larimer County, Colorado, into the North Fork Cache la Poudre River during dam inspections. Approximately 9.6 km of this river was partially or completely affected by this episodic sediment release. Pools up to 3.2 km downstream from the dam lost 50% of their volume. Hess samples taken from October 1996 to September 1997, 100 m downstream from the dam (site 1) and 3.2 km downstream (site 2), revealed effects of sediment on recovery patterns of benthic communities. A 2-way ANOVA was used to determine significant interactions using site and date as main factors. Pairwise differences were then compared using least squares means to determine significant dates within and between sites. Ten days after the sediment release, both density and taxa richness at site 1 (55 organisms per m 2 , 5 taxa) were significantly lower ( P 2 , 25 taxa). These differences remained until June when species richness and densities increased. Plecoptera and Trichoptera colonized from June to September after being eliminated at site 1 and reduced at site 2. No permanently flowing tributaries exist within the study area; therefore, passive downstream drift from such inputs apparently did not influence recovery. Increased densities of taxa such as Baetidae, Hydroptilidae, Hydropsychidae, Chironomidae, Simuliidae, and Oligochaeta occurred plausibly by rapid reproduction. Based on pre-event data, community function completely changed at site 2 from a scraper community to one dominated by collector-gatherers.     

A new antiandrogen. 6α-Bromo-17β-hydroxy-17α-methyl-4-oxa-5α-androstan-3-one

Zusammenfassung Es wird gezeigt, dass 6-Brom-17-hydroxy-17-methyl-4-oxa-5-androstan-3-one beträchtliche antiandrogene Wirkung besitzt. Isomere dieser Verbindung mit verschiedener Konfiguration an C-5 und C-6 wurden als inaktiv befunden.     

Additive two-mode clustering: The error-variance approach revisited

The additive clustering approach is applied to the problem of two-mode clustering and compared with the recent error-variance approach of Eckes and Orlik (1993). Although the schemes of the computational algorithms look very similar in both of the approaches, the additive clustering has been shown to have several advantages. Specifically, two technical limitations of the error-variance approach (see Eckes and Orlik 1993, p. 71) have been overcome in the framework of the additive clustering. The research was supported by the Office of Naval Research under grant number N0014-93-1-0222 to Rutgers University. The authors are indebted both to Fionn Murtagh, who served as Acting Editor, and to anonymous Referees for thoughtful and constructive reviews.     

Location and age of Mesolithic coastal occupation sites on Oronsay, Inner Hebrides

The existence of at least five Mesolithic occupation sites on the island of Oronsay, Inner Hebrides, has now been established. Preliminary results of recent excavation of two of the sites were reported in 1971 (ref. 1). Since then further excavations have been made and the locations and ages of the sites in relation to the position of the shore line of the sea at the maximum of the Holocene marine transgression have been investigated. The results obtained suggest that the ages of some of the sites in relation to the former shore line cannot be established solely on stratigraphical and altitudinal evidence but require supplementary information based on radiocarbon dating. It will be some time before the full details will be published, and so a short account of the results so far obtained is presented now.     

切尔卡斯基及其中国现代诗歌的翻译与研究

本文全面系统地介绍了Л.Е.切尔卡斯基(1925—2003)其人及其中国现代诗歌的翻译与研究。他生于俄国的一个医生之家,中学毕业后,先从军学医,后入莫斯科军译学院攻读汉语。他的最大志趣是中国现代诗歌的翻译与研究。60年代初获苏联科学院中国学研究所副博士学位;70年代初获苏联科学院东方研究所博士学位。其中国现代诗歌翻译与研究学术成果甚丰,在当今世界,堪称中国现代诗歌研究的权威汉学家。     

Matter in Z3

In this paper, I will discuss a certain conception of matter that Aristotle introduces in Metaphysics Z3. It is often assumed that Aristotle came to distinguish between matter and form only in his physical writings, and that this lead to a conflict with the doctrine of primary substances in the Categories that he tries to resolve in Z3. I will argue that there is no such conflict. In Z3, Aristotle seems to suggest that matter is what is left over when we strip a thing of all its properties. I take it that he does not want us to strip away these properties by physical means or in our imagination. Rather, we are asked to strip a referring noun phrase of all its predicative parts. We are thus not supposed to be able to refer to something that has no qualities whatsoever, but to construct a phrase that refers to something that has properties without referring to its having them, and without implying which properties it has. The idea that there might be a way of referring to something definite without mentioning any of its qualities is platonic and it still underlies modern predicate logic. In Z3, Aristotle argues against this conception and thus against the basic idea of predicate logic. According to him, matter is at best an inseparable aspect of a primary substance, which substance is best referred to as a compound τóδε τι (“this such”). Matter is what the τóδε refers to as part of this phrase. But it cannot exist in separation from form, and we cannot refer to it by a separated term, without also referring to the substantial form of the substance of which it is an aspect.