Discovery of a flank caldera and very young glacial activity at Hecates Tholus, Mars

The majority of volcanic products on Mars are thought to be mafic and effusive. Explosive eruptions of basic to ultrabasic chemistry are expected to be common, but evidence for them is rare and mostly confined to very old surface features. Here we present new image and topographic data from the High Resolution Stereo Camera that reveal previously unknown traces of an explosive eruption at 30 degrees N and 149 degrees E on the northwestern flank of the shield volcano Hecates Tholus. The eruption created a large, 10-km-diameter caldera approximately 350 million years ago. We interpret these observations to mean that large-scale explosive volcanism on Mars was not confined to the planet's early evolution. We also show that glacial deposits partly fill the caldera and an adjacent depression. Their age, derived from crater counts, is about 5 to 24 million years. Climate models predict that near-surface ice is not stable at mid-latitudes today, assuming a thermo-dynamic steady state. Therefore, the discovery of very young glacial features at Hecates Tholus suggests recent climate changes. We show that the absolute ages of these very recent glacial deposits correspond very well to a period of increased obliquity of the planet's rotational axis.     

Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2

Autism spectrum disorders comprise a range of neurodevelopmental disorders characterized by deficits in social interaction and communication, and by repetitive behaviour. Mutations in synaptic proteins such as neuroligins, neurexins, GKAPs/SAPAPs and ProSAPs/Shanks were identified in patients with autism spectrum disorder, but the causative mechanisms remain largely unknown. ProSAPs/Shanks build large homo- and heteromeric protein complexes at excitatory synapses and organize the complex protein machinery of the postsynaptic density in a laminar fashion. Here we demonstrate that genetic deletion of ProSAP1/Shank2 results in an early, brain-region-specific upregulation of ionotropic glutamate receptors at the synapse and increased levels of ProSAP2/Shank3. Moreover, ProSAP1/Shank2(-/-) mutants exhibit fewer dendritic spines and show reduced basal synaptic transmission, a reduced frequency of miniature excitatory postsynaptic currents and enhanced N-methyl-d-aspartate receptor-mediated excitatory currents at the physiological level. Mutants are extremely hyperactive and display profound autistic-like behavioural alterations including repetitive grooming as well as abnormalities in vocal and social behaviours. By comparing the data on ProSAP1/Shank2(-/-) mutants with ProSAP2/Shank3αβ(-/-) mice, we show that different abnormalities in synaptic glutamate receptor expression can cause alterations in social interactions and communication. Accordingly, we propose that appropriate therapies for autism spectrum disorders are to be carefully matched to the underlying synaptopathic phenotype.     

Thermal radiation scanning tunnelling microscopy

In standard near-field scanning optical microscopy (NSOM), a subwavelength probe acts as an optical 'stethoscope' to map the near field produced at the sample surface by external illumination. This technique has been applied using visible, infrared, terahertz and gigahertz radiation to illuminate the sample, providing a resolution well beyond the diffraction limit. NSOM is well suited to study surface waves such as surface plasmons or surface-phonon polaritons. Using an aperture NSOM with visible laser illumination, a near-field interference pattern around a corral structure has been observed, whose features were similar to the scanning tunnelling microscope image of the electronic waves in a quantum corral. Here we describe an infrared NSOM that operates without any external illumination: it is a near-field analogue of a night-vision camera, making use of the thermal infrared evanescent fields emitted by the surface, and behaves as an optical scanning tunnelling microscope. We therefore term this instrument a 'thermal radiation scanning tunnelling microscope' (TRSTM). We show the first TRSTM images of thermally excited surface plasmons, and demonstrate spatial coherence effects in near-field thermal emission.     

Intelligent Choice of the Number of Clusters in K-Means Clustering: An Experimental Study with Different Cluster Spreads

The issue of determining “the right number of clusters” in K-Means has attracted considerable interest, especially in the recent years. Cluster intermix appears to be a factor most affecting the clustering results. This paper proposes an experimental setting for comparison of different approaches at data generated from Gaussian clusters with the controlled parameters of between- and within-cluster spread to model cluster intermix. The setting allows for evaluating the centroid recovery on par with conventional evaluation of the cluster recovery. The subjects of our interest are two versions of the “intelligent” K-Means method, ik-Means, that find the “right” number of clusters by extracting “anomalous patterns” from the data one-by-one. We compare them with seven other methods, including Hartigan’s rule, averaged Silhouette width and Gap statistic, under different between- and within-cluster spread-shape conditions. There are several consistent patterns in the results of our experiments, such as that the right K is reproduced best by Hartigan’s rule – but not clusters or their centroids. This leads us to propose an adjusted version of iK-Means, which performs well in the current experiment setting.     

Sperm storage induces an immunity cost in ants

Ant queens are among the most long-lived insects known. They mate early in adult life and maintain millions of viable sperm in their sperm storage organ until they die many years later. Because they never re-mate, the reproductive success of queens is ultimately sperm-limited, but it is not known what selective forces determine the upper limit to sperm storage. Here we show that sperm storage carries a significant cost of reduced immunity during colony founding. Newly mated queens of the leaf-cutting ant Atta colombica upregulate their immune response shortly after completing their nest burrow, probably as an adaptive response to a greater exposure to pathogens in the absence of grooming workers. However, the immune response nine days after colony founding is negatively correlated with the amount of sperm in the sperm storage organ, indicating that short-term survival is traded off against long-term reproductive success. The immune response was lower when more males contributed to the stored sperm, indicating that there might be an additional cost of mating or storing genetically different ejaculates.     

MYC inactivation uncovers pluripotent differentiation and tumour dormancy in hepatocellular cancer

Hepatocellular carcinoma is generally refractory to clinical treatment. Here, we report that inactivation of the MYC oncogene is sufficient to induce sustained regression of invasive liver cancers. MYC inactivation resulted en masse in tumour cells differentiating into hepatocytes and biliary cells forming bile duct structures, and this was associated with rapid loss of expression of the tumour marker alpha-fetoprotein, the increase in expression of liver cell markers cytokeratin 8 and carcinoembryonic antigen, and in some cells the liver stem cell marker cytokeratin 19. Using in vivo bioluminescence imaging we found that many of these tumour cells remained dormant as long as MYC remain inactivated; however, MYC reactivation immediately restored their neoplastic features. Using array comparative genomic hybridization we confirmed that these dormant liver cells and the restored tumour retained the identical molecular signature and hence were clonally derived from the tumour cells. Our results show how oncogene inactivation may reverse tumorigenesis in the most clinically difficult cancers. Oncogene inactivation uncovers the pluripotent capacity of tumours to differentiate into normal cellular lineages and tissue structures, while retaining their latent potential to become cancerous, and hence existing in a state of tumour dormancy.     

Principles of Quantitative Estimation of the Chaperone-Like Activity

IntroductionThe folding of most newly synthesized proteins inthe cell requires the interaction of a variety ofprotein cofactors known as molecular chaperones.These molecules recognize and bind to nascentpolypeptide chains and partially foldedintermediates of proteins,preventing theiraggregation and misfolding[1 7] .　 There are several classes of chaperones definedusing molecular size,cellular compartment andfunction. Thelargestchaperonefamilies are Hsp90(heat shock protein of molecular mass …     

A case of biodiversity overestimation in the Balkan Belgrandiella A. J. Wagner, 1927 (Caenogastropoda: Hydrobiidae): molecular divergence not paralleled by high morphological variation

Belgrandiella A. J. Wagner, 1927 is a genus comprising minute snails with conic or turriform shells that are facultative stygobionts, inhabiting subterranean waters and springs. Few specimens or even empty shells of this taxon can be found, so the taxonomy is based mostly on the shell morphology and geographic distribution. Anatomy is known for a few taxa, and its usefulness is restricted. This, coupled with a popular idea that isolation is the first factor shaping divergence and speciation of the spring/cave fauna, resulted in dozens of nominal species of Belgrandiella, known from shells at a given locality. Herein we present shells, anatomy and four molecular markers (nuclear H3, 18S and 28S and mitochondrial cytochrome oxidase subunit I) in Belgrandiella from 16 localities in Slovenia, northern Croatia, and Bosnia and Herzegovina. Five sites are type localities of Boleana umbilicata, Belgrandiella kusceri, Belgrandiella krupensis, Belgrandiella zermanica and Belgrandiella robusta, respectively. Considering the shell and geographic distribution, we have determined B. cf. robusta Belgrandiella cf. croatica, Belgrandiella cf. fontinalis, Belgrandiella cf. kuesteri, Belgrandiella cf. pageti and Belgrandiella cf. koprivnensis The shells of all, as well as penes and female reproductive organs of two putative, species have been illustrated. Molecular analysis indicates four distinct clades, most probably of species rank for B. kusceri, B. cf. kuesteri, B. cf. fontinalis and B. cf. koprivnensis. Four nominal species (B. krupensis, B. robusta, B. umbilicata and B. zermanica) and one genus (Boleana) must be synonymised with Belgrandiella kusceri, and there are no more than four valid, biological species in the studied Belgrandiella. The molecular divergence is completely unmirrored by high morphological variability. Hence the level of endemism is markedly overestimated in these snails.