Immunological demonstration of large quantities of glycogen or of a glycogen-like substance in human embryonic colon cells and in colon carcinomas by means of rabbit antisera raised against a strain of Escherichia coli 013]

Rabbit antisera raised against a strain of E. coli 013, with a strong antiglycogen activity, were tested on human fetal and normal adult colons, on colon carcinomas, and on colon tumor cells in culture (HT29). Only very rare granules were present in adult normal colons when tested with the immunofluorescence method. In faetal colons, in 12 out of 14 carcinomas, and on HT29 cells, the immunofluorescent reactions were similar to those observed in normal liver. The reactions were negative after previous treatment with alpha-amylase. They were inhibited with glycogen, with phenol-alcohol, perchloric, and trichloroacetic extracts from faetal colons, and with a tumor trichloroacetic extract. The extracts precipitated with anti-E. coli 013 antisera. They had a strong inhibiting activity in a radioimmunoassay test with labeled glycogen. The extracts from normal adult colons did not precipitate with the antisera and they had no inhibiting activity in either immunofluorescence and radioimmunoassay tests.     

Induction of lymphomas by urethane in combination with diethylstilboestrol in CFLP mice.

The combination of urethane and the non-steroidal oestrogen diethylstilboestrol induced lymphomas in CFLP mice.     

The schizogony of Polychromophilus (Haemoproteidae), cosmopolitan parasite of Microchiroptera]

The schizogony of Polychromophilus was partially known by the findings of Schingarew, Mer and Goldblum who demonstrated microschizonts in the reticulo-endothelial tissue. The present work completes these data by showing the presence of macroschizonts in the lungs of both African and European species. These schizonts are characterized by their large size, the hypertrophy of the host cell and its nucleus and the evolution of the parasite apparently slow when young, becoming rapid later.     

Accumulation of metals and mineral particles in the soft tissues of the edible cockle, Cerastoderma edule (Mollusca. Eulamellibranchia). Relation to the environment]

There are two types of metallic accumulations. The first type is constant in localisation and composition. The second type is casually encountered, included in hemocyte nodules, changing with environment and some of its components are incorporated in inorganic particles.     

Increase in the oxygen available to the cerebral cortex after the administration of carbonic anhydrase inhibitors]

Oxygen tension (pO2) in cerebral cortex was measured by polarographic method in unanesthetized rabbits. Intravenous administration (25 mg/kg) of carbonic anhydrase inhibitors (acetazolamide, methazolamide, dichlorphenamide, sulthiame) induced an early important rise of cortical p O2, which is not dependent on increase of p O2 and p CO2 and decrease of pH in arterial blood. High dosage of acetazolamide (250 mg/kg) produced the same effect and did not suppress the increase of cortical p O2 under air-CO2 inhalation. This result suggests that CO2 might act specifically upon cerebral vessels.     

Serotoninergic innervation of the paraventricular organ in chick embryo and chick; histofluorescence and autoradiography study optic and electron microscopy]

Fluorescence microscopy (Falck and Hillarp technique) of 11th day old chick embryo shows aminergic neurons and processes in the paraventricular organ area. Administration of tritiated amines permits us to observe uptake and storage of serotonin only as early as the 10th day; this suggests a very early hypothalamic serotoninergic nervous system differentiation.