Esculentin(1-21), an amphibian skin membrane-active peptide with potent activity on both planktonic and biofilm cells of the bacterial pathogen Pseudomonas aeruginosa

Pseudomonas aeruginosa is an opportunistic bacterial pathogen that forms sessile communities, named biofilms. The non-motile forms are very difficult to eradicate and are often associated with the establishment of persistent infections, especially in patients with cystic fibrosis. The resistance of P. aeruginosa to conventional antibiotics has become a growing health concern worldwide and has prompted the search for new anti-infective agents with new modes of action. Naturally occurring antimicrobial peptides (AMPs) represent promising future template candidates. Here we report on the potent activity and membrane-perturbing effects of the amphibian AMP esculentin(1-21), on both the free-living and sessile forms of P. aeruginosa, as a possible mechanism for biofilm disruption. Furthermore, the findings that esculentin(1-21) is able to prolong survival of animals in models of sepsis and pulmonary infection indicate that this peptide can be a promising template for the generation of new antibiotic formulations to advance care of infections caused by P. aeruginosa.     

High-resolution mapping of meiotic crossovers and non-crossovers in yeast

Meiotic recombination has a central role in the evolution of sexually reproducing organisms. The two recombination outcomes, crossover and non-crossover, increase genetic diversity, but have the potential to homogenize alleles by gene conversion. Whereas crossover rates vary considerably across the genome, non-crossovers and gene conversions have only been identified in a handful of loci. To examine recombination genome wide and at high spatial resolution, we generated maps of crossovers, crossover-associated gene conversion and non-crossover gene conversion using dense genetic marker data collected from all four products of fifty-six yeast (Saccharomyces cerevisiae) meioses. Our maps reveal differences in the distributions of crossovers and non-crossovers, showing more regions where either crossovers or non-crossovers are favoured than expected by chance. Furthermore, we detect evidence for interference between crossovers and non-crossovers, a phenomenon previously only known to occur between crossovers. Up to 1% of the genome of each meiotic product is subject to gene conversion in a single meiosis, with detectable bias towards GC nucleotides. To our knowledge the maps represent the first high-resolution, genome-wide characterization of the multiple outcomes of recombination in any organism. In addition, because non-crossover hotspots create holes of reduced linkage within haplotype blocks, our results stress the need to incorporate non-crossovers into genetic linkage analysis.     

意大利歌剧院的体形、体量及材料对声场的影响

针对意大利歌剧院进行研究，着重研究建筑设计对厅堂音质的影响．对两个剧院的常规音质参量进行了测量．这两座剧院是由同一建筑师Antonio Galli Bibiena设计的，分别是波仑亚的市立剧院和曼图亚的科学剧院．二者具有相当近似的形式，但尺寸不同，所用的材料也各畀，前者是用砖石建成，后者是用木料建成，因此在整修前采用了许多预应力钢筋混凝土结构．文中提供了不少数据并给出了对这些数据加以比较和分析的结果，特别是关于某些特征尺寸与扫频信号各可闻频率产生的空间听觉感受之间相关性的分析，以及若干有关材料和体型对IACC和EDT等音质参量的影响的思考．     

在脉冲响应频率分析比较中对客观声学参数EDT的新认识

对于客观参量EDT，或者更确切地说，对于稳态声场衰变曲线的初始斜率与包括所有简正模式在内的平均阻尼常数之间关系的研究工作，已有一些研究者做过，如H．库特鲁夫就曾指出此参量与所测试环境的几何细节有密切关系．因此，文中选择它作为分析工具，以一种较为深入的方式，通过对一个歌剧院的研究，来检验这种关系．通过对脉冲响应在频域上进行分析，这在前人的工作中也采用过．文中对EDT从倍频程以及1／3倍频程加以研究，并利用在Matlab环境下开发的一些工具来探讨乐池的简正模式与从频带中心频率导出的最大的时间值之间的相关关系．研完表明，在所考察的环境中简正模式与衰变曲线的初始斜率之间有良好的相关关系，与自相关系数（ACF）也有良好的相关关系；研究还证实，用于分析的参量本身既是时间的函数，也是脉冲响应频率变化的函数。     

Iron meteorites as remnants of planetesimals formed in the terrestrial planet region

Iron meteorites are core fragments from differentiated and subsequently disrupted planetesimals. The parent bodies are usually assumed to have formed in the main asteroid belt, which is the source of most meteorites. Observational evidence, however, does not indicate that differentiated bodies or their fragments were ever common there. This view is also difficult to reconcile with the fact that the parent bodies of iron meteorites were as small as 20 km in diameter and that they formed 1-2 Myr earlier than the parent bodies of the ordinary chondrites. Here we show that the iron-meteorite parent bodies most probably formed in the terrestrial planet region. Fast accretion times there allowed small planetesimals to melt early in Solar System history by the decay of short-lived radionuclides (such as 26Al, 60Fe). The protoplanets emerging from this population not only induced collisional evolution among the remaining planetesimals but also scattered some of the survivors into the main belt, where they stayed for billions of years before escaping via a combination of collisions, Yarkovsky thermal forces, and resonances. We predict that some asteroids are main-belt interlopers (such as (4) Vesta). A select few may even be remnants of the long-lost precursor material that formed the Earth.     

Reconciling the temperature dependence of respiration across timescales and ecosystem types

Ecosystem respiration is the biotic conversion of organic carbon to carbon dioxide by all of the organisms in an ecosystem, including both consumers and primary producers. Respiration exhibits an exponential temperature dependence at the subcellular and individual levels, but at the ecosystem level respiration can be modified by many variables including community abundance and biomass, which vary substantially among ecosystems. Despite its importance for predicting the responses of the biosphere to climate change, it is as yet unknown whether the temperature dependence of ecosystem respiration varies systematically between aquatic and terrestrial environments. Here we use the largest database of respiratory measurements yet compiled to show that the sensitivity of ecosystem respiration to seasonal changes in temperature is remarkably similar for diverse environments encompassing lakes, rivers, estuaries, the open ocean and forested and non-forested terrestrial ecosystems, with an average activation energy similar to that of the respiratory complex (approximately 0.65?electronvolts (eV)). By contrast, annual ecosystem respiration shows a substantially greater temperature dependence across aquatic (approximately 0.65?eV) versus terrestrial ecosystems (approximately 0.32?eV) that span broad geographic gradients in temperature. Using a model derived from metabolic theory, these findings can be reconciled by similarities in the biochemical kinetics of metabolism at the subcellular level, and fundamental differences in the importance of other variables besides temperature—such as primary productivity and allochthonous carbon inputs—on the structure of aquatic and terrestrial biota at the community level.     

CDK targets Sae2 to control DNA-end resection and homologous recombination

DNA double-strand breaks (DSBs) are repaired by two principal mechanisms: non-homologous end-joining (NHEJ) and homologous recombination (HR). HR is the most accurate DSB repair mechanism but is generally restricted to the S and G2 phases of the cell cycle, when DNA has been replicated and a sister chromatid is available as a repair template. By contrast, NHEJ operates throughout the cell cycle but assumes most importance in G1 (refs 4, 6). The choice between repair pathways is governed by cyclin-dependent protein kinases (CDKs), with a major site of control being at the level of DSB resection, an event that is necessary for HR but not NHEJ, and which takes place most effectively in S and G2 (refs 2, 5). Here we establish that cell-cycle control of DSB resection in Saccharomyces cerevisiae results from the phosphorylation by CDK of an evolutionarily conserved motif in the Sae2 protein. We show that mutating Ser 267 of Sae2 to a non-phosphorylatable residue causes phenotypes comparable to those of a sae2Delta null mutant, including hypersensitivity to camptothecin, defective sporulation, reduced hairpin-induced recombination, severely impaired DNA-end processing and faulty assembly and disassembly of HR factors. Furthermore, a Sae2 mutation that mimics constitutive Ser 267 phosphorylation complements these phenotypes and overcomes the necessity of CDK activity for DSB resection. The Sae2 mutations also cause cell-cycle-stage specific hypersensitivity to DNA damage and affect the balance between HR and NHEJ. These findings therefore provide a mechanistic basis for cell-cycle control of DSB repair and highlight the importance of regulating DSB resection.     

Terahertz semiconductor-heterostructure laser

Semiconductor devices have become indispensable for generating electromagnetic radiation in everyday applications. Visible and infrared diode lasers are at the core of information technology, and at the other end of the spectrum, microwave and radio-frequency emitters enable wireless communications. But the terahertz region (1-10 THz; 1 THz = 10(12) Hz) between these ranges has remained largely underdeveloped, despite the identification of various possible applications--for example, chemical detection, astronomy and medical imaging. Progress in this area has been hampered by the lack of compact, low-consumption, solid-state terahertz sources. Here we report a monolithic terahertz injection laser that is based on interminiband transitions in the conduction band of a semiconductor (GaAs/AlGaAs) heterostructure. The prototype demonstrated emits a single mode at 4.4 THz, and already shows high output powers of more than 2 mW with low threshold current densities of about a few hundred A cm(-2) up to 50 K. These results are very promising for extending the present laser concept to continuous-wave and high-temperature operation, which would lead to implementation in practical photonic systems.     

Demonstration of temporal cloaking

Recent research has uncovered a remarkable ability to manipulate and control electromagnetic fields to produce effects such as perfect imaging and spatial cloaking. To achieve spatial cloaking, the index of refraction is manipulated to flow light from a probe around an object in such a way that a 'hole' in space is created, and the object remains hidden. Alternatively, it may be desirable to cloak the occurrence of an event over a finite time period, and the idea of temporal cloaking has been proposed in which the dispersion of the material is manipulated in time, producing a 'time hole' in the probe beam to hide the occurrence of the event from the observer. This approach is based on accelerating the front part of a probe light beam and slowing down its rear part to create a well controlled temporal gap--inside which an event occurs--such that the probe beam is not modified in any way by the event. The probe beam is then restored to its original form by the reverse manipulation of the dispersion. Here we present an experimental demonstration of temporal cloaking in an optical fibre-based system by applying concepts from the space-time duality between diffraction and dispersive broadening. We characterize the performance of our temporal cloak by detecting the spectral modification of a probe beam due to an optical interaction and show that the amplitude of the event (at the picosecond timescale) is reduced by more than an order of magnitude when the cloak is turned on. These results are a significant step towards the development of full spatio-temporal cloaking.