Global landscape of HIV-human protein complexes

Human immunodeficiency virus (HIV) has a small genome and therefore relies heavily on the host cellular machinery to replicate. Identifying which host proteins and complexes come into physical contact with the viral proteins is crucial for a comprehensive understanding of how HIV rewires the host's cellular machinery during the course of infection. Here we report the use of affinity tagging and purification mass spectrometry to determine systematically the physical interactions of all 18 HIV-1 proteins and polyproteins with host proteins in two different human cell lines (HEK293 and Jurkat). Using a quantitative scoring system that we call MiST, we identified with high confidence 497 HIV-human protein-protein interactions involving 435 individual human proteins, with ～40% of the interactions being identified in both cell types. We found that the host proteins hijacked by HIV, especially those found interacting in both cell types, are highly conserved across primates. We uncovered a number of host complexes targeted by viral proteins, including the finding that HIV protease cleaves eIF3d, a subunit of eukaryotic translation initiation factor 3. This host protein is one of eleven identified in this analysis that act to inhibit HIV replication. This data set facilitates a more comprehensive and detailed understanding of how the host machinery is manipulated during the course of HIV infection.     

Geophysical evidence from the MELT area for compositional controls on oceanic plates

Magnetotelluric and seismic data, collected during the MELT experiment at the southern East Pacific Rise, constrain the distribution of melt beneath this mid-ocean-ridge spreading centre and also the evolution of the oceanic lithosphere during its early cooling history. Here we focus on structures imaged at distances approximately 100 to 350 km east of the ridge crest, corresponding to seafloor ages of approximately 1.3 to 4.5 million years (Myr), where the seismic and electrical conductivity structure is nearly constant and independent of age. Beginning at a depth of about 60 km, we image a large increase in electrical conductivity and a change from isotropic to transversely anisotropic electrical structure, with higher conductivity in the direction of fast propagation for seismic waves. Conductive cooling models predict structure that increases in depth with age, extending to about 30 km at 4.5 Myr ago. We infer, however, that the structure of young oceanic plates is instead controlled by a decrease in water content above a depth of 60 km induced by the melting process beneath the spreading centre.     

Chaos-based communications at high bit rates using commercial fibre-optic links

Chaotic signals have been proposed as broadband information carriers with the potential of providing a high level of robustness and privacy in data transmission. Laboratory demonstrations of chaos-based optical communications have already shown the potential of this technology, but a field experiment using commercial optical networks has not been undertaken so far. Here we demonstrate high-speed long-distance communication based on chaos synchronization over a commercial fibre-optic channel. An optical carrier wave generated by a chaotic laser is used to encode a message for transmission over 120 km of optical fibre in the metropolitan area network of Athens, Greece. The message is decoded using an appropriate second laser which, by synchronizing with the chaotic carrier, allows for the separation of the carrier and the message. Transmission rates in the gigabit per second range are achieved, with corresponding bit-error rates below 10(-7). The system uses matched pairs of semiconductor lasers as chaotic emitters and receivers, and off-the-shelf fibre-optic telecommunication components. Our results show that information can be transmitted at high bit rates using deterministic chaos in a manner that is robust to perturbations and channel disturbances unavoidable under real-world conditions.     

Numerical Value Results OF Guassian Beam Focussing

1Lens is placed in beam waist We consider the case of a Gaussian beam that is incident at its waist on a thin lens of focal length f.To find the location of the waist of the output beam and the beam radius at that point,we start with the ABCD law.     

The evolution of comets in the Oort cloud and Kuiper belt

Comets are remnants from the time when the outer planets formed, approximately 4-4.5 billion years ago. They have been in storage since then in the Oort cloud and Kuiper belt-distant regions that are so cold and sparsely populated that it was long thought that comets approaching the Sun were pristine samples from the time of Solar System formation. It is now recognized, however, that a variety of subtle but important evolutionary mechanisms operate on comets during their long storage, so they can no longer be regarded as wholly pristine.     

P2X receptors as cell-surface ATP sensors in health and disease

P2X receptors are membrane ion channels activated by the binding of extracellular adenosine triphosphate (ATP). For years their functional significance was consigned to distant regions of the autonomic nervous system, but recent work indicates several further key roles, such as afferent signalling, chronic pain, and in autocrine loops of endothelial and epithelial cells. P2X receptors have a molecular architecture distinct from other ion channel protein families, and have several unique functional properties.     

Targeting abnormal DNA double strand break repair in cancer

A major challenge in cancer treatment is the development of therapies that target cancer cells with little or no toxicity to normal tissues and cells. Alterations in DNA double strand break (DSB) repair in cancer cells include both elevated and reduced levels of key repair proteins and changes in the relative contributions of the various DSB repair pathways. These differences can result in increased sensitivity to DSB-inducing agents and increased genomic instability. The development of agents that selectively inhibit the DSB repair pathways that cancer cells are more dependent upon will facilitate the design of therapeutic strategies that exploit the differences in DSB repair between normal and cancer cells. Here, we discuss the pathways of DSB repair, alterations in DSB repair in cancer, inhibitors of DSB repair and future directions for cancer therapies that target DSB repair.     

Time series forecasts and extra-model information

Often a forecaster has supplementary information (e.g. field reports or forecasts from another source) that cannot be included directly in a time series model. Especially interesting are cases where this information is given at time intervals that are different from those of the time series model forecasts. Previous authors have considered a numerical and a model-based statistical method for combining extra-model information of this type with ARIMA model forecasts. This paper extends both methods to vector ARMA model forecasts and dynamic regression (transfer function) model forecasts. It is also shown that a Lagrange multiplier numerical procedure arises as a special case of the model-based procedure. An empirical example is given.     

Forecasts of power-transformed series

Consider a time series transformed by an instantaneous power function of the Box-Cox type. For a wide range of fractional powers, this paper gives the relative bias in original metric forecasts due to use of the simple inverse retransformation when minimum mean squared error (conditional mean) forecasts are optimal. This bias varies widely according to the characteristics of the data. A fast algorithm is given to find this bias, or to find minimum mean squared error forecasts in the original metric. The results depend on the assumption that the forecast errors in the transformed metric are Gaussian. An example using real data is given.