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81.
Many natural habitats exist on privately owned land outside protected areas, but few governments can afford to enforce or subsidize conservation of this biodiversity. Even in some developed countries, conservation subsidy schemes have only achieved limited success. Fortunately, some landowners may be willing to accept management costs in return for other benefits, although this remains controversial when it involves the killing of charismatic species. For example, participants in British field sports, such as fox hunting and game-bird shooting, may voluntarily conserve important habitats that are required by quarry species. Here we report results from a multidisciplinary study that addressed this issue by focusing on three sites across central England. We found that landowners participating in field sports maintained the most established woodland and planted more new woodland and hedgerows than those who did not, despite the equal availability of subsidies. Therefore, voluntary habitat management appears to be important for biodiversity conservation in Britain. Current debates on the future of field sports in Britain, and similar activities globally, may benefit from considering their utility as incentives to conserve additional habitat on private land. 相似文献
82.
Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing 总被引:26,自引:0,他引:26
Yu G Nishimura M Arawaka S Levitan D Zhang L Tandon A Song YQ Rogaeva E Chen F Kawarai T Supala A Levesque L Yu H Yang DS Holmes E Milman P Liang Y Zhang DM Xu DH Sato C Rogaev E Smith M Janus C Zhang Y Aebersold R Farrer LS Sorbi S Bruni A Fraser P St George-Hyslop P 《Nature》2000,407(6800):48-54
Nicastrin, a transmembrane glycoprotein, forms high molecular weight complexes with presenilin 1 and presenilin 2. Suppression of nicastrin expression in Caenorhabditis elegans embryos induces a subset of notch/glp-1 phenotypes similar to those induced by simultaneous null mutations in both presenilin homologues of C. elegans (sel-12 and hop-1). Nicastrin also binds carboxy-terminal derivatives of beta-amyloid precursor protein (betaAPP), and modulates the production of the amyloid beta-peptide (A beta) from these derivatives. Missense mutations in a conserved hydrophilic domain of nicastrin increase A beta42 and A beta40 peptide secretion. Deletions in this domain inhibit A beta production. Nicastrin and presenilins are therefore likely to be functional components of a multimeric complex necessary for the intramembranous proteolysis of proteins such as Notch/GLP-1 and betaAPP. 相似文献
83.
Single-molecule studies of the effect of template tension on T7 DNA polymerase activity 总被引:8,自引:0,他引:8
T7 DNA polymerase catalyses DNA replication in vitro at rates of more than 100 bases per second and has a 3'-->5' exonuclease (nucleotide removing) activity at a separate active site. This enzyme possesses a 'right hand' shape which is common to most polymerases with fingers, palm and thumb domains. The rate-limiting step for replication is thought to involve a conformational change between an 'open fingers' state in which the active site samples nucleotides, and a 'closed' state in which nucleotide incorporation occurs. DNA polymerase must function as a molecular motor converting chemical energy into mechanical force as it moves over the template. Here we show, using a single-molecule assay based on the differential elasticity of single-stranded and double-stranded DNA, that mechanical force is generated during the rate-limiting step and that the motor can work against a maximum template tension of approximately 34 pN. Estimates of the mechanical and entropic work done by the enzyme show that T7 DNA polymerase organizes two template bases in the polymerization site during each catalytic cycle. We also find a force-induced 100-fold increase in exonucleolysis above 40 pN. 相似文献
84.
DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae 总被引:23,自引:0,他引:23
Heidelberg JF Eisen JA Nelson WC Clayton RA Gwinn ML Dodson RJ Haft DH Hickey EK Peterson JD Umayam L Gill SR Nelson KE Read TD Tettelin H Richardson D Ermolaeva MD Vamathevan J Bass S Qin H Dragoi I Sellers P McDonald L Utterback T Fleishmann RD Nierman WC White O Salzberg SL Smith HO Colwell RR Mekalanos JJ Venter JC Fraser CM 《Nature》2000,406(6795):477-483
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Summer food habits of coyotes ( Canis latrans ) were investigated on a 3100-km 2 area in central Wyoming, divided into one deer-use area and five non-deer areas. Analysis of 404 scats (fecal samples) revealed an overall average of 63 percent occurrence of native ungulates, 63 percent leporids, 46 percent rodents, 14 percent livestock, and 11 percent birds. Pronghorn ( Antilocapra Americana ) was the ungulate most frequently consumed, occurring in about 87 percent of the scats. Mule deer ( Odocoileus hemionus ) occurred in only 8 percent, and in 5 percent the native ungulate remains were not identifiable beyond order. This large percentage of big game in the diet is apparently unusual, because big game has been of minor importance in most coyote food-habit studies. The high incidence of leporids is consistent with other studies performed in arid intermountain areas. Although cricetines, especially deer mice ( Peromyscus maniculatus ), were trapped consistently in all habitats, months, and trapping areas, they were found in scats at a lower frequency than microtines and sciurids. This suggests a coyote hunting strategy that selected for the latter two groups. 相似文献
88.
K J Mikolajczak R V Madrigal J K Rupprecht Y H Hui Y M Liu D L Smith J L McLaughlin 《Experientia》1990,46(3):324-327
Sylvaticin (I), a new tetrahydroxy annonaceous acetogenin with nonadjacent tetrahydrofuran rings, has been isolated from the dried fruits of Rollinia sylvatica St. Hil. (Annonaceae). This compound is extremely cytotoxic to human tumor cells and shows promising insect control properties. 相似文献
89.
Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells 总被引:8,自引:0,他引:8
Human tumours often contain DNA sequences not found in normal tissues which are able to transform cultured NIH 3T3 cells. In some tumours the gene responsible for this transformation belongs to the cellular ras gene family. A specific type of mutation is responsible for converting the cellular proto-oncogene into a ras oncogene capable of inducing transformation. In a study of the function of a cellular ras gene, its protein product (produced in a bacterial cell) was microinjected into NIH 3T3 cells; the recipient cells became morphologically transformed and were induced to initiate DNA synthesis in the absence of added serum, but only when cellular ras protein was injected at much higher concentrations than required with protein of the transforming ras gene. To further analyse the function of the cellular ras gene, we have now injected monoclonal antibodies against ras proteins into NIH 3T3 cells. We report here that NIH 3T3 cells induced to divide by adding serum to the culture medium are unable to enter the S phase of the cell cycle after microinjection of anti-ras antibody, showing that the protein product of the ras proto-oncogene is required for initiation of the S-phase in NIH 3T3 cells. 相似文献
90.
To aid the conservation and management of Mountain Plovers ( Charadrius montanus ), we developed a spatially explicit model of breeding habitat for the shrub-steppe of western Wyoming. Points of Mountain Plover presence and absence were determined by field surveys conducted May through June 1999. At each point we measured topographic slope and a cover type suitability index related to vegetation height and density. Logistic regression revealed a strong negative relationship between presence and slope, and a weaker and positive relationship between presence and cover index. A multiple logistic regression model using slope and cover index to predict presence successfully classified 87% of the points in an independent data set covering much of western Wyoming. The spatial expression of this model will help managers target future surveys and identify currently unsuitable habitat that could be improved via vegetation management. Patches of suitable breeding habitat of the Mountain Plover in western Wyoming are probably functions of poor soil, low precipitation, and wind scour, and thus are likely rather persistent in space and time. Combined with the relatively large proportion of publicly owned land here, this may make conservation and recovery of the Mountain Plover easier in western Wyoming than in the more dynamic and privately controlled Great Plains. 相似文献