全文获取类型
收费全文 | 34533篇 |
免费 | 107篇 |
国内免费 | 144篇 |
专业分类
系统科学 | 147篇 |
丛书文集 | 497篇 |
教育与普及 | 65篇 |
理论与方法论 | 109篇 |
现状及发展 | 15085篇 |
研究方法 | 1467篇 |
综合类 | 16804篇 |
自然研究 | 610篇 |
出版年
2013年 | 239篇 |
2012年 | 495篇 |
2011年 | 1109篇 |
2010年 | 211篇 |
2008年 | 581篇 |
2007年 | 717篇 |
2006年 | 639篇 |
2005年 | 706篇 |
2004年 | 736篇 |
2003年 | 613篇 |
2002年 | 659篇 |
2001年 | 1091篇 |
2000年 | 1050篇 |
1999年 | 742篇 |
1994年 | 356篇 |
1992年 | 705篇 |
1991年 | 511篇 |
1990年 | 566篇 |
1989年 | 529篇 |
1988年 | 519篇 |
1987年 | 545篇 |
1986年 | 583篇 |
1985年 | 772篇 |
1984年 | 548篇 |
1983年 | 449篇 |
1982年 | 398篇 |
1981年 | 414篇 |
1980年 | 470篇 |
1979年 | 1123篇 |
1978年 | 873篇 |
1977年 | 808篇 |
1976年 | 670篇 |
1975年 | 658篇 |
1974年 | 914篇 |
1973年 | 760篇 |
1972年 | 850篇 |
1971年 | 945篇 |
1970年 | 1306篇 |
1969年 | 1000篇 |
1968年 | 901篇 |
1967年 | 885篇 |
1966年 | 877篇 |
1965年 | 636篇 |
1959年 | 320篇 |
1958年 | 624篇 |
1957年 | 426篇 |
1956年 | 328篇 |
1955年 | 299篇 |
1954年 | 344篇 |
1948年 | 237篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
951.
L Feliubadaló M Font J Purroy F Rousaud X Estivill V Nunes E Golomb M Centola I Aksentijevich Y Kreiss B Goldman M Pras D L Kastner E Pras P Gasparini L Bisceglia E Beccia M Gallucci L de Sanctis A Ponzone G F Rizzoni L Zelante M T Bassi A L George M Manzoni A De Grandi M Riboni J K Endsley A Ballabio G Borsani N Reig E Fernández R Estévez M Pineda D Torrents M Camps J Lloberas A Zorzano M Palacín 《Nature genetics》1999,23(1):52-57
952.
A I den Hollander J B ten Brink Y J de Kok S van Soest L I van den Born M A van Driel D J van de Pol A M Payne S S Bhattacharya U Kellner C B Hoyng A Westerveld H G Brunner E M Bleeker-Wagemakers A F Deutman J R Heckenlively F P Cremers A A Bergen 《Nature genetics》1999,23(2):217-221
Retinitis pigmentosa (RP) comprises a clinically and genetically heterogeneous group of diseases that afflicts approximately 1.5 million people worldwide. Affected individuals suffer from a progressive degeneration of the photoreceptors, eventually resulting in severe visual impairment. To isolate candidate genes for chorioretinal diseases, we cloned cDNAs specifically or preferentially expressed in the human retina and the retinal pigment epithelium (RPE) through a novel suppression subtractive hybridization (SSH) method. One of these cDNAs (RET3C11) mapped to chromosome 1q31-q32.1, a region harbouring a gene involved in a severe form of autosomal recessive RP characterized by a typical preservation of the para-arteriolar RPE (RP12; ref. 3). The full-length cDNA encodes an extracellular protein with 19 EGF-like domains, 3 laminin A G-like domains and a C-type lectin domain. This protein is homologous to the Drosophila melanogaster protein crumbs (CRB), and denoted CRB1 (crumbs homologue 1). In ten unrelated RP patients with preserved para-arteriolar RPE, we identified a homozygous AluY insertion disrupting the ORF, five homozygous missense mutations and four compound heterozygous mutations in CRB1. The similarity to CRB suggests a role for CRB1 in cell-cell interaction and possibly in the maintenance of cell polarity in the retina. The distinct RPE abnormalities observed in RP12 patients suggest that CRB1 mutations trigger a novel mechanism of photoreceptor degeneration. 相似文献
953.
The identification of genes that control susceptibility to testicular germ-cell tumours (TGCTs), the most common cancer affecting young men, has been difficult. In laboratory mice, TGCTs arise from primordial germ cells in only the 129 inbred strains, and susceptibility is under multigenic control. The spontaneously arising mutation Ter (ref. 5) on mouse chromosome 18 (Refs 6,7) increases TGCT frequency on a 129/Sv background. We originally used Ter in genetic crosses to identify loci that control tumorigenesis. A genome scan of tumour-bearing progeny from backcrosses between the 129/Sv-Ter/+ and MOLF/Ei strains provided modest evidence that MOLF-derived alleles on chromosome 19 enhance development of bilateral TGCTs (ref. 9). To obtain independent evidence for linkage to the MOLF chromosome, we made an autosomal chromosome substitution strain (CSS; or 'consomic strain') in which chromosome 19 of 129/Sv+/+ was replaced by its MOLF-derived homologue. The unusually high frequency of TGCTs in this CSS (even in the absence of the Ter mutation) provides evidence confirming the genome survey results, identifies linkage for a naturally occurring strain variant allele that confers susceptibility to TGCTs and illustrates the power of CSSs in complex trait analysis. 相似文献
954.
Minisatellites are tandemly repeated DNA sequences of 10-100-bp units. Some minisatellite loci are highly unstable in the human germ line, and structural analysis of mutant alleles has suggested that repeat instability results from a recombination-based process. To provide insights into the molecular mechanism of human minisatellite instability, we developed Saccharomyces cerevisiae strains carrying alleles of the most unstable human minisatellite locus, CEB1 (ref. 2). We observed that CEB1 is destabilized in meiosis, resulting in a variety of intra- and inter-allelic gains or losses of repeat units, similar to rearrangements described in humans. Using mutations affecting the initiation of recombination (spo11) or mismatch repair (msh2 pms1 ), we demonstrate that meiotic destabilization depends on the initiation of homologous recombination at nearby DNA double-strand break (DSBs) sites and involves a 'rearranged heteroduplex' intermediate. Most of the human and yeast data can be explained and unified in the context of DSB repair models. 相似文献
955.
采用形变热处理技术有效地提高改良型T91 耐热钢的高温回火抗力和室温综合力学性能。该钢经形变热处理后性能提高的主要机制是细晶强化和伴随晶粒细化而产生的马氏体组织的细化以及析出物的弥散分布。 相似文献
956.
Direct multicriteria clustering algorithms 总被引:1,自引:0,他引:1
In a multicriteria clustering problem, optimization over more than one criterion is required. The problem can be treated in
different ways: by reduction to a clustering problem with the single criterion obtained as a combination of the given criteria;
by constrained clustering algorithms where a selected critetion is considered as the clustering criterion and all others determine
the constraints; or by direct algorithms. In this paper two types of direct algorithms for solving multicriteria clustering
problem are proposed: the modified relocation algorithm, and the modified agglomerative algorithm. Different elaborations
of these two types of algorithms are discussed and compared. Finally, two applications of the proposed algorithms are presented.
Elaborated version of the talks presented at the First Conference of the International Federation of Classification Societies,
Aachen, 1987, at the International Conference on Social Science Methodology, Dubrovnik, 1988, and at the Second Conference
of the International Federation of Classification Societies, Charlottesville, 1989.
This work was supported in part by the Research Council of Slovenia. 相似文献
957.
Single linkage clusters on a set of points are the maximal connected sets in a graph constructed by connecting all points
closer than a given threshold distance. The complete set of single linkage clusters is obtained from all the graphs constructed
using different threshold distances. The set of clusters forms a hierarchical tree, in which each non-singleton cluster divides
into two or more subclusters; the runt size for each single linkage cluster is the number of points in its smallest subcluster.
The maximum runt size over all single linkage clusters is our proposed test statistic for assessing multimodality. We give
significance levels of the test for two null hypotheses, and consider its power against some bimodal alternatives.
Research partially supported by NSF Grant No. DMS-8617919. 相似文献
958.
The spontaneously hypertensive rat (SHR) is an important animal model of human essential hypertension. During the first month of life, increased retention of sodium is present in the SHR which appears to be mediated by the renin-angiotensin system. The present review will discuss the role that increased activity of the renin-angiotensin system plays in sodium/body fluid regulation during early development. It is hypothesized that disordered regulation of sodium/body fluid homeostasis during this stage leads to pathological cardiovascular regulation in adulthood. Through an understanding of the relationship between sodium/body fluid balance in the young and cardiovascular function in the adult insights may be gained into both the pathological state of hypertension and the critical role played by early development in shaping homeostatic mechanisms in adulthood. 相似文献
959.
S. Steghaus-Kovâc U. Maschwitz A. B. Attygalle R. T. S. Frighetto N. Frighetto O. Vostrowsky H. J. Bestmann 《Cellular and molecular life sciences : CMLS》1992,48(7):690-694
Behavioral tests carried out with the four stereoisomers of 4-methyl-3-heptanol revealed thatLeptogenys diminuta ants respond specifically only to the (3R, 4S)-isomer. 相似文献
960.
Y. Sakagami K. Taki T. Matsuhisa S. Marumo 《Cellular and molecular life sciences : CMLS》1992,48(8):793-795
2-Deoxyecdysone was purified from the miteTyrophagus putrescentiae and monitored by a radioimmunoassay. The compound was identified by GC-MS and LC-MS. This is the first report of an endogenous ecdysteroid from a mite. 相似文献