Fluorescence of indole derivatives

Zusammenfassung Die Verwendung von 1N Natronlauge und Essigsäureanhydrid bei der Bestimmung von Indolderivaten mit Hilfe der Fluoreszenzmethode erlaubt eine gute Differenzierung zwischen 5-Methoxyindolderivaten (z.B. Melatonin) und anderen Indolen. Versuche zur Verwendung der neuen Methode in biologischem Material, z.B. Epiphysenschnitten, sind im Gange.     

Purification and properties of a heat-resistant exotoxin produced byMacrophomina phaseolina (Tassi) Goid in culture

Summary A partially purified preparation of a water-soluble, heat-resistant, nonspecific exotoxin produced by a strain ofMacrophomina phaseolina, isolated fromPhaseolus mungo L. could reduce Cu⁺⁺ ions and produced a red colour with 2,4-dinitrophenyl hydrazine reagent. It caused inhibition of seed germination, wilting of cut seedlings, stunted growth of young seedlings and loss of permeability of the cell membrane. Seedlings ofP. mungo, grown in presence of the toxin showed a slight increase in the contents of protein and total RNA over control, but a significant increase in the specific activities of F-1, 6-BP aldolase and G-6-P isomerase.     

Lactose binding to heat-labile enterotoxin revealed by X-ray crystallography.

Recognition of the oligosaccharide portion of ganglioside GM1 in membranes of target cells by the heat-labile enterotoxin from Escherichia coli is the crucial first step in its pathogenesis, as it is for the closely related cholera toxin. These toxins have five B subunits, which are essential for GM1 binding, and a single A subunit, which needs to be nicked by proteolysis and reduced, yielding an A1-'enzyme' and an A2-'linker' peptide. A1 is translocated across the membrane of intestinal epithelial cells, possibly after endocytosis, upon which it ADP-ribosylates the G protein Gs alpha. The mechanism of binding and translocation of these toxins has been extensively investigated, but how the protein is orientated on binding is still not clear. Knowing the precise arrangement of the ganglioside binding sites of the toxins will be useful for designing drugs against the diarrhoeal diseases caused by organisms secreting these toxins and in the development of oral vaccines against them. We present here the three-dimensional structure of the E. coli heat-labile enterotoxin complexed with lactose. This reveals the location of the binding site of the terminal galactose of GM1, which is consistent with toxin binding to the target cell with its A1 fragment pointing away from the membrane. A small helix is identified at the carboxy terminus of A2 which emerges through the central pore of the B subunits and probably comes into contact with the membrane upon binding, whereas the A1 subunit is flexible with respect to the B pentamer.     

A smart automated macroeconometric forecasting system

A smart, automated forecasting system is a kind of expert system for generating forecasts wholly or partly without human intervention. A pilot-scale system is reported in this paper. The interventions are made by a rulebase which describes the actual intervention procedures of the London Business School Centre for Economic Forecasting on one of the major macroeconometric forecasting models for the U.K. economy. The rulebase is sufficiently general as to be applicable to any forecasting model. One difference of the pilot model reported in this paper from conventional models is that policy behaviour is described entirely by rules rather than equations. This allows the use of thresholds, floors, ceilings, and discontinuities.