全文获取类型
收费全文 | 431篇 |
免费 | 2篇 |
国内免费 | 2篇 |
专业分类
系统科学 | 13篇 |
丛书文集 | 1篇 |
理论与方法论 | 19篇 |
现状及发展 | 77篇 |
研究方法 | 56篇 |
综合类 | 249篇 |
自然研究 | 20篇 |
出版年
2021年 | 4篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2016年 | 8篇 |
2015年 | 5篇 |
2014年 | 3篇 |
2013年 | 8篇 |
2012年 | 32篇 |
2011年 | 47篇 |
2010年 | 17篇 |
2009年 | 4篇 |
2008年 | 38篇 |
2007年 | 45篇 |
2006年 | 31篇 |
2005年 | 25篇 |
2004年 | 21篇 |
2003年 | 33篇 |
2002年 | 25篇 |
2001年 | 4篇 |
2000年 | 6篇 |
1999年 | 2篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1994年 | 3篇 |
1992年 | 1篇 |
1991年 | 4篇 |
1990年 | 4篇 |
1989年 | 7篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1977年 | 5篇 |
1976年 | 2篇 |
1975年 | 5篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1971年 | 2篇 |
1970年 | 2篇 |
1968年 | 2篇 |
1967年 | 3篇 |
1966年 | 1篇 |
1962年 | 2篇 |
1961年 | 1篇 |
1960年 | 1篇 |
1946年 | 1篇 |
排序方式: 共有435条查询结果,搜索用时 0 毫秒
21.
22.
23.
Loss-of-function mutations in LEMD3 result in osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis 总被引:11,自引:0,他引:11
Hellemans J Preobrazhenska O Willaert A Debeer P Verdonk PC Costa T Janssens K Menten B Van Roy N Vermeulen SJ Savarirayan R Van Hul W Vanhoenacker F Huylebroeck D De Paepe A Naeyaert JM Vandesompele J Speleman F Verschueren K Coucke PJ Mortier GR 《Nature genetics》2004,36(11):1213-1218
Osteopoikilosis, Buschke-Ollendorff syndrome (BOS) and melorheostosis are disorders characterized by increased bone density. The occurrence of one or more of these phenotypes in the same individual or family suggests that these entities might be allelic. We collected data from three families in which affected individuals had osteopoikilosis with or without manifestations of BOS or melorheostosis. A genome-wide linkage analysis in these families, followed by the identification of a microdeletion in an unrelated individual with these diseases, allowed us to map the gene that is mutated in osteopoikilosis. All the affected individuals that we investigated were heterozygous with respect to a loss-of-function mutation in LEMD3 (also called MAN1), which encodes an inner nuclear membrane protein. A somatic mutation in the second allele of LEMD3 could not be identified in fibroblasts from affected skin of an individual with BOS and an individual with melorheostosis. XMAN1, the Xenopus laevis ortholog, antagonizes BMP signaling during embryogenesis. In this study, LEMD3 interacted with BMP and activin-TGFbeta receptor-activated Smads and antagonized both signaling pathways in human cells. 相似文献
24.
25.
26.
Rawat UB Zavialov AV Sengupta J Valle M Grassucci RA Linde J Vestergaard B Ehrenberg M Frank J 《Nature》2003,421(6918):87-90
Protein synthesis takes place on the ribosome, where genetic information carried by messenger RNA is translated into a sequence of amino acids. This process is terminated when a stop codon moves into the ribosomal decoding centre (DC) and is recognized by a class-1 release factor (RF). RFs have a conserved GGQ amino-acid motif, which is crucial for peptide release and is believed to interact directly with the peptidyl-transferase centre (PTC) of the 50S ribosomal subunit. Another conserved motif of RFs (SPF in RF2) has been proposed to interact directly with stop codons in the DC of the 30S subunit. The distance between the DC and PTC is approximately 73 A. However, in the X-ray structure of RF2, SPF and GGQ are only 23 A apart, indicating that they cannot be at DC and PTC simultaneously. Here we show that RF2 is in an open conformation when bound to the ribosome, allowing GGQ to reach the PTC while still allowing SPF-stop-codon interaction. The results indicate new interpretations of accuracy in termination, and have implications for how the presence of a stop codon in the DC is signalled to PTC. 相似文献
27.
Jayantha Arnold Arvind Sangwaiya Vijay Manglam Frank Geoghegan Mark Thursz Mark Busbridge 《河南科技》2010,(3)
AIM: To examine body fluids such as ascitic fluid (AF),saliva,bile and pleural effusions for the presence of hepcidin using a novel radioimmunoassay (RIA).METHODS: Serum samples were collected from 25 healthy volunteers (mean age: 36 ± 11.9 years,11 males,14 females).In addition bile was obtained from 12 patients undergoing endoscopic retrograde cholangiopancreatography (mean age: 66.9 ± 16.7 years,M:F = 5:7).Saliva was collected from 17 healthy volunteers (mean age: 35 ± 9.9 years,M:F = 8:9).Pleural and AF... 相似文献
28.
29.
Khajetoorians AA Chilian B Wiebe J Schuwalow S Lechermann F Wiesendanger R 《Nature》2010,467(7319):1084-1087
An individual magnetic atom doped into a semiconductor is a promising building block for bottom-up spintronic devices and quantum logic gates. Moreover, it provides a perfect model system for the atomic-scale investigation of fundamental effects such as magnetism in dilute magnetic semiconductors. However, dopants in semiconductors so far have not been studied by magnetically sensitive techniques with atomic resolution that correlate the atomic structure with the dopant's magnetism. Here we show electrical excitation and read-out of a spin associated with a single magnetic dopant in a semiconductor host. We use spin-resolved scanning tunnelling spectroscopy to measure the spin excitations and the magnetization curve of individual iron surface-dopants embedded within a two-dimensional electron gas confined to an indium antimonide (110) surface. The dopants act like isolated quantum spins the states of which are governed by a substantial magnetic anisotropy that forces the spin to lie in the surface plane. This result is corroborated by our first principles calculations. The demonstrated methodology opens new routes for the investigation of sample systems that are more widely studied in the field of spintronics-that is, Mn in GaAs (ref. 5), magnetic ions in semiconductor quantum dots, nitrogen-vacancy centres in diamond and phosphorus spins in silicon. 相似文献
30.
Genome sequence and analysis of the tuber crop potato 总被引:11,自引:0,他引:11
Potato Genome Sequencing Consortium Xu X Pan S Cheng S Zhang B Mu D Ni P Zhang G Yang S Li R Wang J Orjeda G Guzman F Torres M Lozano R Ponce O Martinez D De la Cruz G Chakrabarti SK Patil VU Skryabin KG Kuznetsov BB Ravin NV Kolganova TV Beletsky AV Mardanov AV Di Genova A Bolser DM Martin DM Li G Yang Y Kuang H Hu Q Xiong X Bishop GJ Sagredo B Mejía N Zagorski W Gromadka R Gawor J Szczesny P Huang S Zhang Z Liang C He J Li Y He Y Xu J Zhang Y Xie B Du Y Qu D Bonierbale M Ghislain M 《Nature》2011,475(7355):189-195
Potato (Solanum tuberosum L.) is the world's most important non-grain food crop and is central to global food security. It is clonally propagated, highly heterozygous, autotetraploid, and suffers acute inbreeding depression. Here we use a homozygous doubled-monoploid potato clone to sequence and assemble 86% of the 844-megabase genome. We predict 39,031 protein-coding genes and present evidence for at least two genome duplication events indicative of a palaeopolyploid origin. As the first genome sequence of an asterid, the potato genome reveals 2,642 genes specific to this large angiosperm clade. We also sequenced a heterozygous diploid clone and show that gene presence/absence variants and other potentially deleterious mutations occur frequently and are a likely cause of inbreeding depression. Gene family expansion, tissue-specific expression and recruitment of genes to new pathways contributed to the evolution of tuber development. The potato genome sequence provides a platform for genetic improvement of this vital crop. 相似文献