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91.
Mueller JL Mahadevaiah SK Park PJ Warburton PE Page DC Turner JM 《Nature genetics》2008,40(6):794-799
According to the prevailing view, mammalian X chromosomes are enriched in spermatogenesis genes expressed before meiosis and deficient in spermatogenesis genes expressed after meiosis. The paucity of postmeiotic genes on the X chromosome has been interpreted as a consequence of meiotic sex chromosome inactivation (MSCI)--the complete silencing of genes on the XY bivalent at meiotic prophase. Recent studies have concluded that MSCI-initiated silencing persists beyond meiosis and that most genes on the X chromosome remain repressed in round spermatids. Here, we report that 33 multicopy gene families, representing approximately 273 mouse X-linked genes, are expressed in the testis and that this expression is predominantly in postmeiotic cells. RNA FISH and microarray analysis show that the maintenance of X chromosome postmeiotic repression is incomplete. Furthermore, X-linked multicopy genes exhibit a similar degree of expression as autosomal genes. Thus, not only is the mouse X chromosome enriched for spermatogenesis genes functioning before meiosis, but in addition, approximately 18% of mouse X-linked genes are expressed in postmeiotic cells. 相似文献
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The epiphyseal cartilage from new-born mouse was treated with collagenase in two ways: either before fixation or after glutaraldehyde fixation. The electron dense granules of the matrix were not seen in the micrographs of cartilage treated with collagenase before fixation. It is concluded that collagen plays a definite role in the formation of the granules at the time of tissue fixation and that the granules are fixation artifacts. 相似文献
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Summary Intravenous injection of sodium succinate in dogs anesthetized with chloralosane produces a prolonged stimulation of respiration and decreases the duration of the apnea following pulmonary hyperventilation. 相似文献
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Summary Suppression of the chemo-receptors of the carotid sinus and aorta æreas prolongs the acapnic apnea markedly. Animals deprived of their chemo-receptors may even die in apnea.During hyperventilation, the expiratory position of the thorax increases. During the acapnic apnea the inspiratory position returns progressively to normal. During hyperventilation and acapnic apnea, rhythmic changes occur in the tonus of the respiratory muscles. These changes in tonus occur in normal animals as well as in animals deprived of their chemo-receptors and vagi nerves.In normal dogs, hyperventilation and acapnea do not induce a fall in arterial blood pressure. Arterial hypotension occurs, however, during hyperventilation in dogs deprived of their carotid sinus and aortic innervation. During acapnic apnea, the arterial blood pressure rises in animals deprived of their carotid sinus and aortic nerves. A secondary fall of arterial pressure occurs during prolonged acapnic apnea in these animals. 相似文献
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H Jakob P Dubois H Eisen F Jacob 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1978,286(1):109-111
HMBA induces differentiation in the whole population of some multipotential embryonic carcinoma cells. Morphological, biochemical and immunological changes can be observed even after short treatment. The cells lose the embryonal F9 antigen without acquiring H-2 antigens. 相似文献
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