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61.
Dictyostelium discoideum cells shed vesicles with associated DNA and vital stain Hoechst 33342 总被引:2,自引:0,他引:2
I. Tatischeff M. Bomsel C. de Paillerets H. Durand B. Geny D. Segretain E. Turpin A. Alfsen 《Cellular and molecular life sciences : CMLS》1998,54(5):476-487
Dictyostelium discoideum cells are highly resis tant to xenobiotics. We previously observed that these primitive eukaryotic cells contain a 170-kDa
P-glycoprotein, mediating multidrug resistance in mammalian cells, but nonfunctional in Dictyostelium cells. We show here that D. discoideum cells vitally stained with the DNA-specific dye, Hoechst 33342, release fluorescent material in their culture medium. Electron
microscopy and lipid analysis demonstrate the vesicular nature of this material. Moreover, nucleic acids associate with these
extracellular vesicles independently of Hoechst vital staining. The main vesicular DNA component exhibits a size >21 kb. Shedding
of microvesicles during cell growth is not concomitant with programmed cell death. We propose that these extracellular vesicles
are involved in a new cellular resistance mechanism against xenobiotics. Furthermore, since the association of DNA with vesicles
occurs in physiological growth conditions and independently of vital staining, the new shedding process might be involved
in a more general intercellular mechanism.
Received 14 November 1997; received after revision 16 March 1998; accepted 16 March 1998 相似文献
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P. Scarmato C. Cherqui-Eisenberg G. Durand J. Feger 《Cellular and molecular life sciences : CMLS》1989,45(5):480-482
Summary The binding of asialoglycoproteins by hepatic binding protein was studied in freshly isolated hepatocytes from genetically diabetic BB Wistar rats. The number of cell surface asialoglycoprotein receptors was dramatically decreased (58,000±38,000 for diabetic rats compared to 267,000±70,000 for normal rats), while the association equilibrium constant was not changed. These results parallel those obtained with streptozotocin-diabetic rats and support the hypothesis that insulin deprivation is responsible for the decrease in the receptor number. 相似文献
66.
R. Durand 《Cellular and molecular life sciences : CMLS》1982,38(3):341-342
Summary Illumination during the photoinhibited phase of the development ofCoprinus congregatus reduced fruiting. This inhibitory effect of light was dependent on the radiant flux density and on temperature. Radiant fluxes far lower than the flux provided by moonlight elicited photoresponses. 相似文献
67.
F Desnoyers G Durand 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1978,287(4):357-359
After thymidine-3H injection into pregnant Rat, histoautoradiographic studies showed that adipose cell proliferation in different subcutaneous and visceral sites, was very important in Rat foetuses about 24 h before birth and that lipid accumulation in the labelled cells was very fast during perinatal 48 h. 相似文献
68.
The binding of asialoglycoproteins by hepatic binding protein was studied in freshly isolated hepatocytes from genetically diabetic BB Wistar rats. The number of cell surface asialoglycoprotein receptors was dramatically decreased (58,000 +/- 38,000 for diabetic rats compared to 267,000 +/- 70,000 for normal rats), while the association equilibrium constant was not changed. These results parallel those obtained with streptozotocin-diabetic rats and support the hypothesis that insulin deprivation is responsible for the decrease in the receptor number. 相似文献