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971.
972.
Astrocytes induce blood-brain barrier properties in endothelial cells 总被引:53,自引:0,他引:53
The highly impermeable tight junctions between endothelial cells forming the capillaries and venules in the central nervous system (CNS) of higher vertebrates are thought to be responsible for the blood-brain barrier that impedes the passive diffusion of solutes from the blood into the extracellular space of the CNS. The ability of CNS endothelial cells to form a blood-brain barrier is not intrinsic to these cells but instead is induced by the CNS environment: Stewart and Wiley demonstrated that when avascular tissue from 3-day-old quail brain is transplanted into the coelomic cavity of chick embryos, the chick endothelial cells that vascularize the quail brain grafts form a competent blood-brain barrier; on the other hand, when avascular embryonic quail coelomic grafts are transplanted into embryonic chick brain, the chick endothelial cells that invade the mesenchymal tissue grafts form leaky capillaries and venules. It is, however, not known which cells in the CNS are responsible for inducing endothelial cells to form the tight junctions characteristic of the blood-brain barrier. Astrocytes are the most likely candidates since their processes form endfeet that collectively surround CNS microvessels. In this report we provide direct evidence that astrocytes are capable of inducing blood-brain barrier properties in non-neural endothelial cells in vivo. 相似文献
973.
974.
Inequality in mutation rates of the two strands of DNA 总被引:4,自引:0,他引:4
As the mechanisms for replicating the two strands of duplex DNA differ it is, in principle, possible for the mutation rates to differ depending on which strand is being copied. In the absence of selection this would lead to a difference in the measured rate of a particular base substitution, such as T to C, depending on which DNA strand was analysed to determine the rate. Thus a change such as T to C on one DNA strand results from either a direct T-to-C mutation on that strand or an A-to-G mutation on the complementary strand; for the other strand the situation is reversed, and it can be seen that different processes are responsible for the two cases, allowing for asymmetry in substitution rate. We have tested whether such asymmetry indeed occurs by studying equivalent sequences from the beta-globin complexes of six species of primate. Our results reveal an asymmetry in substitution rates consistent with predictions based on strand-inequalities in mutation rates. Our sequence comparisons also allow us to make predictions about the positions of replication origins and the replication error rates of one strand relative to the other. 相似文献
975.
Protein kinases mediate nitric oxide-induced apoptosis in the insect cell line IPLB-LdFB 总被引:2,自引:0,他引:2
The involvement of protein kinases (PKA, PKC and PKB) in nitric oxide (NO)-induced apoptosis with sodium nitroprusside plus
N-acetyl-L-cysteine in the IPLB-LdFB cell line from the insect Lymantria dispar was investigated. The presence of protein kinase-like molecules was demonstrated by western blot analysis. The role of the
kinases in programmed cell death was analysed in cytofluorimetric experiments by incubating the insect cells with H-89 (a
specific inhibitor of PKA), calphostin C (an inhibitor of PKC) or wortmannin (an inhibitor of phosphatidylinositol 3-kinase).
The results show that PKA is correlated with the induction and PKC and PKB with the prevention of NO-induced insect cell death.
Moreover, NO-induced apoptosis involves the release of cytochrome c.
Received 15 March 2002; accepted 25 March 2002 相似文献
976.
RAGE is a multiligand receptor of the immunoglobulin superfamily: implications for homeostasis and chronic disease 总被引:19,自引:0,他引:19
Bucciarelli LG Wendt T Rong L Lalla E Hofmann MA Goova MT Taguchi A Yan SF Yan SD Stern DM Schmidt AM 《Cellular and molecular life sciences : CMLS》2002,59(7):1117-1128
Receptor for AGE (RAGE) is a member of the immunoglobulin superfamily that engages distinct classes of ligands. The biology of RAGE is driven by the settings in which these ligands accumulate, such as diabetes, inflammation, neurodegenerative disorders and tumors. In this review, we discuss the context of each of these classes of ligands, including advance glycation end-products, amyloid beta peptide and the family of beta sheet fibrils, S100/calgranulins and amphoterin. Implications for the role of these ligands interacting with RAGE in homeostasis and disease will be considered. 相似文献
977.
Plant thioredoxins: the multiplicity conundrum 总被引:4,自引:0,他引:4
Thioredoxins are small proteins distinguished by the presence of a conserved dicysteine active site. In oxidized thioredoxin,
the two cysteines form a disulfide bond that is targeted by the enzyme thioredoxin reductase. Together with an electron donor,
thioredoxin and thioredoxin reductase form the 'thioredoxin system' that is present in all organisms. Thioredoxins participate
in dithiol/disulfide exchange reactions with a large range of cellular substrates. Higher plants possess a very complex thioredoxin
profile consisting of at least two different thioredoxin systems that contain distinct, multigenic thioredoxin classes which
have different intracellular localizations. In this review we summarise the current state of knowledge regarding the function
of plant thioredoxins representing all systems and classes.
Received 30 October 2001; received after revision 13 December 2001; accepted 17 December 2001 相似文献
978.
Screening for differentially expressed genes is a straightforward approach to study the molecular basis for changes in gene
expression. Differential display analysis has been used by investigators in diverse fields of research since it was developed.
Differential display has also been the approach of choice to investigate changes in gene expression in response to various
biological challenges in invertebrates. We review the application of differential display analysis of gene expression in invertebrates,
and provide a specific example using this technique for novel gene discovery in the nematode Caenorhabditis elegans. 相似文献
979.
Morphine 6 glucuronide stimulates nitric oxide release in mussel neural tissues: evidence for a morphine 6 glucuronide opiate receptor subtype 总被引:1,自引:0,他引:1
Mantione K Zhu W Rialas C Casares F Cadet P Franklin AL Tonnesen J Stefano GB 《Cellular and molecular life sciences : CMLS》2002,59(3):570-574
We have previously demonstrated that Mytilus edulis pedal ganglia contain opiate alkaloids, i.e., morphine and morphine 6 glucuronide (M6G), as well as mu opiate receptor subtype
fragments exhibiting high sequence similarity to those found in mammals. Now we demonstrate that M6G stimulates pedal ganglia
constitutive nitric oxide (NO) synthase (cNOS)-derived NO release at identical concentrations and to similar peak levels as
morphine. However, the classic opiate antagonist, naloxone, only blocked the ability of morphine to stimulate cNOS-derived
NO release and not that of M6G. CTOP, a mu-specific antagonist, blocked the ability of M6G to induce cNOS-derived NO release
as well as that of morphine, suggesting that a novel mu opiate receptor was present and selective toward M6G. In examining
a receptor displacement analysis, both opiate alkaloids displaced [3H]-dihydromorphine binding to the mu opiate receptor subtype. However, morphine exhibited a twofold higher affinity, again
suggesting that a novel mu opiate receptor may be present.
Received 1 November 2001; received after revision 1 February 2002; accepted 1 February 2002 相似文献
980.
The Ror receptor tyrosine kinase family 总被引:6,自引:0,他引:6
Forrester WC 《Cellular and molecular life sciences : CMLS》2002,59(1):83-96
Receptor tyrosine kinases (RTKs) participate in numerous developmental decisions. Ror RTKs are a family of orphan receptors
that are related to muscle specific kinase (MuSK) and Trk neurotrophin receptors. MuSK assembles acetylcholine receptors at
the neuromuscular junction [1, 2], and Trk receptors function in the developing nervous system (reviewed in [3-5]). Rors have
been identified in nematodes, insects and mammals. Recent studies have begun to shed light on Ror function during development.
In most species, Rors are expressed in many tissue types during development. Analyses of mutants that are defective in the
single nematode Ror demonstrate a role in cell migration and in orienting cell polarity. Mice lacking one of the two Ror gene
products display defects in bone and heart formation. Similarly, two different human bone development disorders, dominant
brachydactyly B and recessive Robinow syndrome, result from mutations in one of the human Ror genes.
Received 17 April 2001; received after revision 2 July 2001; accepted 4 July 2001 相似文献