Glycerylphosphorylarsenocholine and phosphatidylarsenocholine in yelloweye mullet (Aldrichetta forsteri) following oral administration of arsenocholine

Summary The novel arsenical glycerylphosphorylarsenocholine has been isolated from yelloweye mullet (Aldrichetta forsteri) following oral administration of arsenocholine. Ether-soluble arsenic was shown to be present as phosphatidylarsenocholine.     

Inhibition of in vitro RNA synthesis by hycanthone,oxamniquine and praziquantel

Summary The schistosomicides, hycanthone, oxamniquine and praziquantel, were found to inhibit the in vitro RNA synthesis using isolated hamster liver nuclei. Preincubation of the nuclei with these drugs revealed that the inhibitory effect of oxamniquine was irreversible and progressed with time, whereas that of hycanthone and parziquantel was reversible. On the other hand, hycanthone and praziquantel have a high affinity for DNA but oxamniquine does not. The data indicate that the mechanism of inhibition by oxamniquine is different from that of hycanthone and praziquantel.     

Frequent mutation of histone-modifying genes in non-Hodgkin lymphoma

Follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL) are the two most common non-Hodgkin lymphomas (NHLs). Here we sequenced tumour and matched normal DNA from 13 DLBCL cases and one FL case to identify genes with mutations in B-cell NHL. We analysed RNA-seq data from these and another 113 NHLs to identify genes with candidate mutations, and then re-sequenced tumour and matched normal DNA from these cases to confirm 109 genes with multiple somatic mutations. Genes with roles in histone modification were frequent targets of somatic mutation. For example, 32% of DLBCL and 89% of FL cases had somatic mutations in MLL2, which encodes a histone methyltransferase, and 11.4% and 13.4% of DLBCL and FL cases, respectively, had mutations in MEF2B, a calcium-regulated gene that cooperates with CREBBP and EP300 in acetylating histones. Our analysis suggests a previously unappreciated disruption of chromatin biology in lymphomagenesis.     

Human-specific loss of regulatory DNA and the evolution of human-specific traits

Humans differ from other animals in many aspects of anatomy, physiology, and behaviour; however, the genotypic basis of most human-specific traits remains unknown. Recent whole-genome comparisons have made it possible to identify genes with elevated rates of amino acid change or divergent expression in humans, and non-coding sequences with accelerated base pair changes. Regulatory alterations may be particularly likely to produce phenotypic effects while preserving viability, and are known to underlie interesting evolutionary differences in other species. Here we identify molecular events particularly likely to produce significant regulatory changes in humans: complete deletion of sequences otherwise highly conserved between chimpanzees and other mammals. We confirm 510 such deletions in humans, which fall almost exclusively in non-coding regions and are enriched near genes involved in steroid hormone signalling and neural function. One deletion removes a sensory vibrissae and penile spine enhancer from the human androgen receptor (AR) gene, a molecular change correlated with anatomical loss of androgen-dependent sensory vibrissae and penile spines in the human lineage. Another deletion removes a forebrain subventricular zone enhancer near the tumour suppressor gene growth arrest and DNA-damage-inducible, gamma (GADD45G), a loss correlated with expansion of specific brain regions in humans. Deletions of tissue-specific enhancers may thus accompany both loss and gain traits in the human lineage, and provide specific examples of the kinds of regulatory alterations and inactivation events long proposed to have an important role in human evolutionary divergence.     

Potential for controlling the spread of Centaurea maculosa with grass competition

Spotted knapweed ( Centaurea maculosa Lam.) is a major rangeland and roadside weed of the northern Rocky Mountains. It is often found in plant communities dominated by Pseudoroegneria spicatum or Festuca idahoensis , but it rarely invades roadsides dominated by Bromus tnermis Leyss. Aboveground biomass of the 3 grass species grown in mixture with Centaurea was compared to growth in monoculture at a range of nitrogen input levels. The results suggest that Bromus is capable of suppressing the growth of Centaurea with the degree of suppression increasing with increasing nitrogen levels. The 2 native grasses had no impact on Centaurea under the controlled environment conditions of this study.     

Selection of big sagebrush by sage grouse

Feeding sites of wintering sage grouse ( Centrocercus urophasianus ) were located, one each in stands of three subspecies of big sagebrush ( Artemisia tridentata : ssp. tridentata , basin; ssp. vaseyana , mountain; and ssp. Wyomingensis , Wyoming). Evidences of differential use of plants within subspecies were observed. Whole leaves from fed-on and nonfed-on big sagebrush plants were examined for intrasubspecies chemical comparisons of crude protein, phosphorus, in vitro digestibility, and monoterpenoids. No significant differences were detected except for in vitro digestibility of Wyoming fed-on and nonfed-on big sagebrush and monoterpenoid content of basin big sagebrush. Nutritive content of all three subspecies was high, which may in part help to explain wintering sage grouse weight gains.     

Parasites of the bowhead whale, Balaena mysticetus

Blood, tissue, and organ samples from five bowhead whales were examined for ecto- and endoparasites. Two species of protozoans, four genera of diatoms, one species of trematoda, two species of nematoda, and one species of amphipoda "louse" were found. No blood parasites were recovered. The larval anisakid nematode, found in the submucosa of the forestomach of one whale, generated a prominent inflammatory response. Protozoans found in contents of the colon included a flagellate and a sarcodinan. The sarcodinan, which was common in the colon contents of one whale, belongs to the genus Entamoeba and probably represents an undescribed species. Ogomogaster plicatus , a trematode, was also identified. The data from this study are compared with previous lists of parasites for the bowhead whale and two other species of baleen whales. From the results presented, the previous list of parasites for the bowhead whale has been expanded to include eight additional genera and species.