Platelet-derived growth factor receptor beta identifies mesenchymal stem cells with enhanced engraftment to tissue injury and pro-angiogenic property

Mesenchymal stem cells (MSCs) are heterogeneous likely consisting of subpopulations with various therapeutic potentials. Here we attempted to acquire a subset of MSCs with enhanced effect in wound healing. We found that human placental MSCs expressing platelet-derived growth factor (PDGF) receptor (PDGFR)-β exhibited greater proliferation rates and generated more colony-forming unit-fibroblast (CFU-F), compared to PDGFR-β^? MSCs. Notably, PDGFR-β⁺ MSCs expressed higher levels of pro-angiogenic factors such as Ang1, Ang2, VEGF, bFGF and PDGF. When 10⁶ GFP-expressing MSCs were topically applied into excisional wounds in mice, PDGFR-β⁺ MSCs actively incorporated into the wound tissue, resulting in enhanced engraftment (3.92 ± 0.31 × 10⁵ remained in wound by 7 days) and accelerated wound closure; meanwhile, PDGFR-β^? MSCs tended to remain on the top of the wound bed with significantly fewer cells (2.46 ± 0.26 × 10⁵) engrafted into the wound, suggesting enhanced chemotactic migration and engraftment of PDGFR-β⁺ MSCs into the wound. Real-Time PCR and immunostain analyses revealed that the expression of PDGF-B was upregulated after wounding; transwell migration assay showed that PDGFR-β⁺ MSCs migrated eightfold more than PDGFR-β^? MSCs toward PDGF-BB. Intriguingly, PDGFR-β⁺ MSC-treated wounds showed significantly enhanced angiogenesis compared to PDGFR-β^? MSC- or vehicle-treated wounds. Thus, our results indicate that PDGFR-β identifies a subset of MSCs with enhanced chemotactic migration to wound injury and effect in promoting angiogenesis and wound healing, implying a greater therapeutic potential for certain diseases.     

MetaOJ: A Massive Distributed Online Judge System

Online Judge(OJ) systems are a basic and important component of computer education. Here, we present MetaOJ, an OJ system that can be used for holding massive programming tests online. MetaOJ is designed to create a distributed, fault-tolerant, and easy-to-scale OJ system from an existing ordinary OJ system by adding several interfaces into it and creating multiple instances of it. Our case on modifying the TUOJ system shows that the modification adds no more than 3% lines of code and the performance loss on a single OJ instance is no more than 12%. We also introduce mechanisms to integrate the system with cloud infrastructure to automate the deployment process. MetaOJ provides a solution for those OJ systems that are designed for a specific programming contest and are now facing performance bottlenecks.     

2D perovskites for field-effect transistors

Metal halide perovskites have gained a lot of attention in the field of solar cells and light-emitting diodes because of their excellent optoelectronic properti...     

Act in contravention: a non-planar coupled electrode design utilizing "tip effect" for ultra-high areal capacity,long cycle life zinc-based batteries

Aqueous zinc-based batteries (ZBBs) have great potential as commercial energy storage devices.However,the poor cycling stability of zinc anode under high areal ...     

指标均衡性对评价结果的影响

本文针对一类需要考虑指标间均衡性的决策问题,利用Gini系数,提出了一种新的多目标决策方法。     

CADCSC软件系统人机界面设计

本文论述了CADCSC软件系统的界面设计。提出了适合各种用户的、由多种交互方式组成的混合人机交互界面;为控制系统的计算机辅助设计提供了统一的命令表达方式和统一的运行界面;介绍了其中界面设计一致性与灵活性。     

Adaptive Asymptotic Tracking Control for Stochastic Nonlinear Systems with Unknown Backlash-Like Hysteresis

In this study, an adaptive asymptotic tracking control problem is considered for stochastic nonlinear systems with unknown backlash-like hysteresis. By utilizing backstepping technology and bound estimation approach, an adaptive asymptotic tracking control scheme is designed, where fuzzy systems are applied to approximate unknown function terms, the effect of hysteresis and stochastic disturbances is compensated appropriately. The proposed scheme ensures that the tracking error can asymptoticall...