重楼活性单体PP-26抑制结肠癌SW620细胞增殖并诱导细胞凋亡

目的:探讨重楼活性单体PP-26对人结肠癌SW620细胞增殖抑制作用及其机制.方法:采用噻唑蓝(MTT)法和克隆形成抑制实验观察不同浓度的重楼单体PP-26对人结肠癌SW620细胞增殖抑制作用;PI单染及Annexin V-FITC/PI双染流式细胞术检测细胞周期变化及细胞凋亡水平;Western blotting检测PP-26对细胞周期、细胞凋亡相关蛋白以及Akt和ERK蛋白的表达.结果:与正常肝LO2细胞相比,重楼单体PP-26能显著抑制SW620细胞的生长,作用呈剂量-效应关系;随着PP-26浓度的增加,细胞克隆形成逐渐减少,与细胞对照组相比有显著差异;不同浓度PP-26作用后,细胞阻滞于G1期;PP-26作用细胞24 h后,CDK4、CDK6表达下降,P15、cyclin D1表达增加;不同浓度PP-26作用后,细胞晚期凋亡率增加,随浓度增加有上升趋势;PP-26作用细胞24 h后,线粒体相关凋亡信号通路蛋白Caspase-9、Caspase-3表达下降,PARP切割条带增加,细胞促凋亡蛋白Bax的表达增加,抗凋亡蛋白Bcl-2和Bcl-x L减少,p-Akt和p-ERK蛋白表达均下降.结论:重楼活性单体PP-26通过上调p15促进结肠癌SW620细胞阻滞于G1期,通过抑制P13K/Akt信号通路及ERK信号通路,活化线粒体凋亡通路,诱导细胞凋亡.     

求解线性约束凸规划问题的预估校正内点法

提出一个求解线性约束凸规划问题的预估校正内点法,方法对初始迭代点的可行性没有任何要求,并证明了所给方法等价于1阶拢动复合牛顿法,且给出了一些数值试验结果。     

Steinitz class of elliptic curves with complex multilication

Let E be any elliptic curve having complex multiplication by the ring CK of integers of the quadratic number field K= Q(- D). Let H be the Hilbert class field of K. The Mordell-Weil group E(H) of H-rational points is a module over the Dedekind domain CK, its structure depends on its Steinitz class. Here the Steinits class is determined when D is any prime number. This result advances the result for the specific elliptic curves when D=10.A general theorem on structure of modules over Dedekind domain is also proposed.     

自抗扰精密跟踪运动控制器的设计

为了提高非圆车削中快速伺服刀架的跟踪精度和抗干扰性,研制了一种自抗扰控制器。通过对执行机构建模分析,将模型中的非线性部分归结为系统的内扰,将加工过程中切削力干扰归结为系统外扰,设计出相应的扩张状态观测器,对系统内、外扰的总和做出实时估计和补偿。仿真实验表明,自抗扰控制性能优于传统的PID控制。通过DSP(digital signal processor)编程,将自抗扰控制器应用于非圆车削。切削加工试验结果表明,自抗扰控制具有良好的控制品质,快速伺服刀架跟踪精度控制在±5μm以内。     

Diagnosis of Multiple Fixture Faults in Multiple-Station Manufacturing Processes Based on State Space Approach

Dimensional quality is one of the most critical challenges in industries, which uses the multistage manufacturing process (MMP) such as assembly and machining for automotive and aerospace industries.According to investigations, fixture faults accounted for 72% of all the dimensional faults. Previous studies focused on only one fault or multiple faults occurred in one station or one fault in multiple stations, but these cases rarely appear in the real manufacturing. This paper presents a method for diagnosis of multiple fixture faults in the multi-station manufacturing process. The proposed method is based on the state space model of the MMP processes, which carries the information of the fixture layout geometry and sensor position. To identify the root cause, three continuous steps were used: a) development of the state space model and the construction of the statistics variables on offline mode, b) measurement of the coordinate measuring machines data on online mode and calculation of the statistics variables, and c) diagnostic algorithm for identifying the root cause. The presented paper integrates the state space model of the manufacturing processes and hypothesis test considering the impact of the measure noises. A case study verifies the proposed method.     

Effect of thrombin on the apoptosis of hippocampal neurons in vitro

Hippocampal neurons were treated by thrombin and thrombin receptor activating peptides (TRAP). Cell survival rate was decreased in a dose-dependent manner by MTT assay. The numbers of apoptotic cell and apoptotic rate of hippocampal neurons treated by different concentrations of thrombin were increased in a dose-dependent manner by terminal deoxynucleotidyl transferase (TdT) mediated dUTP-biotin nick end-labeling (TUNEL) method and Flow Cytometry. When the concentration of thrombin is 40 U/mL, TUNEL positive cells and apoptotic rate of hippocampal neurons reached peak value, were 27.3±4.0 and (29.333±4.633)%, respectively. Immunocytochemistry assay show that Bcl-2 protein expression was down-regulated and Bax protein expression was up-regulated with the concentration of thrombin increased. TRAP can mimic the effect of thrombin to induce apoptosis on hippocampal neurons. These data demonstrated that thrombin induced hippocampal neuron apoptosis in a dose-dependent manner through activating protease-activated protein-1 (PAR-1). The change in expression of Bcl-2 and Bax was related with the effect of high concentration thrombin induced apoptosis on hippocampal neurons. Foundation item: Supported by the Natural Science Foundation of Hainan Province (N30215) Biography: YANG Wen-qiong (1968-), female, Ph.D. candidate, research direction: cerebrovascular disease.     

不同生物炭进入土壤后对304不锈钢腐蚀的影响

生物炭在土壤应用过程,不可避免地会对与土壤之间接触的机械设备部分产生腐蚀作用。为探究生物炭应用于农业生产中对机械设备以及金属工具损耗的影响,采用分别在700、400和100℃温度下裂解制备的小麦秸秆生物炭(WB)、水稻秸秆生物炭(RB)和松木生物炭(PB)的生物炭对304不锈钢板材进行腐蚀处理,并测量其极化曲线和电化学阻抗谱和腐蚀表现。结果表明:与空白组(CK)对比,相较于304不锈钢的腐蚀速率,在施入WB的土壤中,腐蚀速率会随着WB裂解温度的增大而增大;而加入RB的土壤会加剧不锈钢的腐蚀,其中在加入400℃RB的土壤中腐蚀速率达到最大;这是因为WB和RB的加入会提升土壤中的Cl~-含量至足以达到点蚀效应的程度加速了不锈钢板材的腐蚀。与此同时,发现100℃制备的PB生物炭可以抑制不锈钢的腐蚀。总而言之,不同生物质和制备温度的生物炭在土壤实际应用中表现出不同的腐蚀和抗腐蚀特性,因此深入研究这些方面将为其农业应用具有深远影响。     

空心莲子草吸附钴离子前后溶液pH值的变化及其红外光谱研究

用PH计和红外光谱研究了空心莲子草对Co(NO3)2的生物吸附特性，发现空心莲子草对Co^2 具有有较强的吸附能力，在达到饱和吸附前，其吸附量随Co^2 的起始浓度的增加而增加，吸附前的PH值及红外光谱表明；在吸附过程中，羟基和氨基起重要作用，Co^2 与生物材料中的氢（可能是羟基氨）发生了交换，且化学吸附和物理吸附同时起作用。     

短沟道离子注入GaAs MESFET解析模型

本文提出了一种新的离子注入短沟道GaAsMESFET直流解析模型,并将解析模型与实验数据进行了比较。结果表明,本文提出的解析模型可以同时描述高、中和低夹断电压的短沟道离子注入GaAs MESFET直流特性.     

内藏钢桁架带洞口混凝土组合核心筒抗震试验及分析

提出了内藏钢桁架带洞口混凝土组合核心筒,该组合核心筒包含2种组合,即不同受力体系桁架与核心筒的组合、不同材料型钢与混凝土的组合,形成了双重组合核心筒.对2个1/6缩尺的带洞口核心筒结构模型进行了低周反复荷载下的抗震性能试验研究,其中包括一个普通带洞口混凝土核心筒和一个内藏钢桁架带洞口混凝土组合核心筒.在试验的基础上,对比分析了2个试件的刚度及其衰减过程、承载力、延性、滞回特性、耗能及破坏特征.试验及分析表明,内藏钢桁架带洞口混凝土组合核心筒比普通带洞口混凝土核心筒抗震能力明显提高.建立了内藏钢桁架带洞口混凝土组合核心筒的承载力计算模型,计算结果与实测值符合较好.提出了该新型组合核心筒抗震设计的建议.