In-situ observation on the deformation behaviors of Fe-Mn-C TWIP steel

The microstructure and crack behaviour of twinning induced plasticity (TWIP) steel during tensile deformation was investigated with in-situ scanning electron microscopy (SEM). The results show that there are two modes of plastic deformation during tensile test in the Fe-Mn-C TWIP steel: dislocation gliding and deformation twins. During the process of tensile deformation, secondary deformed twins are found. Inclusions have played a role in the course of ductile fracture, and microcracks initiate from inclusions and twin-twin intersections.     

Research and development of 780 MPa cold rolling TRIP-aided steel

As an industry-university cooperative project, an experimental research was conducted to develop a new cold-rolled transformation-induced plasticity (TRIP) steel with a tensile strength of 780 MPa and an elongation of 25% under the conditions that some processing parameters were preestablished according to the actual conditions of factory production lines. The optimal heat treatment conditions for obtaining excellent strength-ductility balance, specifically in intercritical annealing temperature and isothermal treatment temperature, were investigated by means of thermal simulation tests. Ultimately the desirable mechanical properties were attained successfully, and the microstructure and retained austenite stability were studied by optical microscopy (OM) and electron back-scattered diffraction (EBSD). It has been discovered that the sizes of retained austenite grains are generally proportional to the sizes of carbon-clear space (ferrite and bainite) around them, and there is marked selectivity in their transition sequences under stress-strain.     

积极健康老龄化导向的西藏老年服务产业发展研究

实施积极的健康老龄化战略,确保所有老年人“老有所养、病有所医、老有所乐、老有所为”是老年群体公平分享社会经济发展成果的诉求,也是不断改善民生、增进国民福利的保障。运筹发展西藏老年服务产业不仅能应对即将到来的“少子老龄化”社会带来的一系列棘手问题,使老年人能健康快乐地安度晚年,以促进代际、代内公平和社会和谐。而且,老年服务产业在实现养老风险社会化分担从而减轻家庭养老压力的同时,还能创造大量的就业岗位和拉动社会需求。为此,文章提出西藏老年服务产业发展的基本思路：政府支持、战略牵引、需求导向、人文关怀、社会参与。     

Thermal ablation versus conventional regional hyperthermia has greater anti-tumor activity against melanoma in mice by upregulating CD4~+ cells and enhancing IL-2 secretion

To determine whether conventional hyperthermia (42-45℃) or ablation therapy (＞50℃) achieves better synergistic effects on direct cytotoxicity and anti-tumor immunity,we compared the therapeutic effects of two hyperthermia temperatures,43 and 55℃,in terms of cytotoxicity and upregulation of immune functions in a mouse malignant melanoma model.Melanoma-bearing mice were treated by directly applying regional hyperthermia to the tumor nodule with a heating light at a temperature of 43℃ for 30 min or 55℃ for 10 min.The tumor growth curve and mice survival rate were observed.To investigate the hyperthermia-induced immunological response,peripheral blood CD4~+ and CD8~+ T cells and the serum IL-2 level were determined.Our results indicated that application of regional hyperthermia at the ablation temperature (such as 55℃) achieved better synergistic anti-tumor effects than did conventional hyperthermia (43℃).Significant increases in the number of peripheral blood CD4~+ T cells and the serum IL-2 level likely contributed to the underlying mechanism.     

Production of human lysozyme-transgenic cloned porcine embryos by somatic nuclear transfer

Due to their physiology and organ size, pigs have significant potential as human disease models and as organ transplantation donors. Genetic modification of pigs could provide benefits for both agriculture and human medicine. In this study, five fetal pig fibroblast cell lines from two species (Wuzhishan and Landrace pigs) were transfected using double-marked human lysozyme (HLY) plasmids (pBC1-HLY-GFP-NEO) by a liposome-mediated method. The ratio of green fluorescent protein (GFP)-expressing cells was >95% in sw7, sw8, slw3 and slw6 cell lines, but only 49.3% in slw9 cells. Cells from the four highly transgenic lines were used as nuclear donors to construct embryos, which were then cultured after fusion and activation by electric stimulation. The rate of cleavage was 76.7%, 48 h after activation. After 7 days, 18.5% of cleaved eggs had developed to the blastocyst stage and 93.3% of blastocysts were GFP-positive. These results indicate that transgenic fetal pig fibroblast cell lines could be obtained by a liposome-mediated method, though the transfection efficiency varied between cell lines. Reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive.     

湖南省科技中介服务发展现状及其发展建议

一个功能较完善、机构充分发展、服务质量较高的科技中介服务体系,是区域创新体系不可或缺的重要组成部分,是推进地区新型工业化、构建特色区域知识经济体系的重要支撑。当前,湖南科技中介机构的发展与创新需求还存在很大差距,认识不到位、体系不完善、基础薄弱、服务质量不高、社会信誉度偏低、相关政策法规体系不完善等问题较突出。加强科技中介机构的自身服务能力建设,进一步规范政府部门的引导职能,努力构建湖南特色新型科技中介服务体系是建设创新型湖南的迫切需要,是加速湖南特色新型工业化进程的重要保障。     

不同温度条件下氟乐灵对茄子黄萎病的诱抗效果

研究了诱抗剂氟乐灵在不同温度条件下对茄子黄萎病的诱抗效果.结果表明,氟乐灵对茄子黄萎病具有较好的诱抗效果,持效期在24d以上.温度对诱抗效果和持效期具有显著影响,氟乐灵在20℃和25℃诱抗防效较佳,持效期长;而30℃时诱抗效果较差,持效期较短.控制适宜的温度可以提高诱抗剂的防效,并延长持效期.     

政府在高职教育校企合作中的功能定位和作为

在公共服务型政府的职能框架下,分析校企合作困境中的政府因素,研究得出政府在高职院校校企合作中应担负起健全机制、建立法规、加强管理的职能,并对校企合作过程进行组织协调、扶持保障、责任监督和对校企合作成果进行评价激励的结论,为校企合作中政府的功能定位和作为提供参考。     

人本主义教育思想与体育教学方法改革

本文分析了在当前体育教学改革新形势下的体育教学方法中的一些问题,提出应在人本主义教育思想指导下,进行体育教学语法改革。     

Enhancive effect of N,N′-dinitrosopiperazine on inducing precancerous lesion on nasal and/or nasopharyngeal epithelia of TgN(p53mt-LMP1)/HT mice

Objective: To investigate the enhancive effect of N,N′-dinitrosopiperazine (DNP) on induced carcinogenesis in nasal and/or nasopharyngeal epithelia among TgN(p53mt-LMP1)/HT transgenic mice to examine the underlying mechanism for the development of nasopharyngeal carcinoma (NPC). Methods: TgN(p53mt-LMP1)/HT transgenic mice and the same strain of C₅₇BL/6J wild-type mice both at the age of 5 months were randomly divided into 2 groups in parallel, respectively, i.e., TgN(p53mt-LMP1)/HT cancerous lesion-inducing group (TI), TgN(p53mt-LMP1)/HT control group (TC), C₅₇BL/6J cancerous lesion-inducing group (CI), and C₅₇BL/6J control group (CC). TI and CI mice were treated only with DNP for 16 weeks, twice each week, while TC and CC mice were given the same volume of saline as controls. At the end of treatment, animals were sacrificed to collect epithelial tissue samples from nasal cavity and nasopharynx for pathohistological evaluation by haematoxylin and eosin (HE) staining and for determination on the expression of TRAF2, c-Jun, and p16 by immunohistochemistry. Results: Atypical hyperplasia was more significant in the samples of TI than in those of TC, CI, and CC, with the rates of lesions being 90%, 10%, 0, and 0 (P<0.01) respectively, though DNP was used alone in a much shortened inducing period at less dosage and without the use of carcinogenic promoter 12-O-tetradecanoylphorbol-13-acetate as usual. The expressions of tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2) and c-Jun in these samples were significantly up-regulated in TI (P<0.01), while the expression of p16 was significantly lower in TI than in the other groups (P<0.01). Conclusion: TgN(p53mt-LMP1)/HT mice hold inherited constitutional defect in immune surveillance function, which can be aggravated by environmental carcinogens, such as DNP used even though in a much less strength. The enhanced carcinogenesis-inducing effect of DNP on TgN(p53mt-LMP1)/HT mice should be closely associated with abnormal signaling of activator protein-1 (AP-1) pathway, especially up-regulated expressions of TRAF2 and c-Jun, and down-regulated expression of p16.