胆总管探查切口的Ⅰ期缝合

目的 :分析胆囊切除术胆总管探查阴性病例的处理方法。方法 :回顾性分析 5 5 2例良性病胆囊切除术连续病例。结果 :胆总管探查 380例中 15 1例探查阴性 ,其中 31例胆总管 期缝合 ,12 0例置 T管引流。结论 :胆总管探查阴性病例在术中胆道镜仔细检查的条件下 ,胆总管探查切口 期缝合是安全的。     

文蛤(Meretrix meretrix)地理种群ISSR分子标记的初步研究

利用ISSR(InterSimpleSequenceRepeate)技术对文蛤(Meretrixmeretrix)江苏和辽宁两个地理种群进行了PCR扩增.从100条ISSR引物中筛选出引物13条,每条引物检测出位点数1到8.平均每条引物可检测到位点数4 6个.实验结果表明:江苏文蛤的位点多态性(80 7%)高于辽宁文蛤的位点多态性(68 4%);种群内平均遗传距离分别为0 3105±0 090和0 2658±0 044,江苏文蛤也高于辽宁文蛤.从筛选得到的引物可以发现,文蛤简单重复序列中A、G碱基含量较高.通过对不同实验条件的对比,对ISSR PCR反应体系进行了优化.     

次声信息和磁场对黑曲霉的生物学效应

次声信息和磁场对黑曲霉的生物学效应赖素聪，郑忠辉，沈持衡，徐伟明（生物学系）（电子工程系）次声的频率很低（２０Ｈｚ以下），人耳听不见，自然界中广泛存在，它的穿透力很强，能引起生物体的生物效应￣［１～３］；磁场对生物体也有作用，曾有学者作过这方面的研究...     

磁头定位系统温度特性的仿真研究

用计算机仿真研究磁头定位系统的性能随温度变化的特性，与测试分析相结合，得出了有关宽温带高精度磁头定位系统设计的一些有意义的结论．     

水跃消能对闸基渗流稳定的影响分析

水闸泄洪时,下泄水流的势能转化为动能,导致出闸水流的流速增加和水面下降,从而形成附加渗透水头．研究了闸孔泄洪条件下水跃区附加渗透水头对闸室基底渗流稳定的影响,结果表明：为保证工程安全,在进行闸基的防渗布置时不仅要考虑静态渗透水头的作用,还应考虑下泄水流水位变化所引起的附加渗透水头对闸基渗流稳定的影响．提出了减小附加渗透水头的工程措施,冒水孔应设置于消力池末端,而将消力池前段设为不透水底板,以利用跃后高水来提高渗流出逸点的压力水头,从而达到减小附加渗透水头的目的．     

T形线散射电流的麦克斯韦电路

运用最新提出的麦克斯韦电路理论,分析T形线的散射电流,将该结构等效为LC电路,求解与之对应的微分方程,得到该结构的散射电流,数值实验分别计算了T形线的等效电路参数L,C,α,β,以及相应的散射电流,分析结果表明,所得电路参数和散射电流符合物理现象,并且与矩量法的结果以及相关文献中的结果均吻合,从而证明了该方法的正确性和...     

Prion-like behaviour and tau-dependent cytotoxicity of pyroglutamylated amyloid-β

Extracellular plaques of amyloid-β and intraneuronal neurofibrillary tangles made from tau are the histopathological signatures of Alzheimer's disease. Plaques comprise amyloid-β fibrils that assemble from monomeric and oligomeric intermediates, and are prognostic indicators of Alzheimer's disease. Despite the importance of plaques to Alzheimer's disease, oligomers are considered to be the principal toxic forms of amyloid-β. Interestingly, many adverse responses to amyloid-β, such as cytotoxicity, microtubule loss, impaired memory and learning, and neuritic degeneration, are greatly amplified by tau expression. Amino-terminally truncated, pyroglutamylated (pE) forms of amyloid-β are strongly associated with Alzheimer's disease, are more toxic than amyloid-β, residues 1-42 (Aβ(1-42)) and Aβ(1-40), and have been proposed as initiators of Alzheimer's disease pathogenesis. Here we report a mechanism by which pE-Aβ may trigger Alzheimer's disease. Aβ(3(pE)-42) co-oligomerizes with excess Aβ(1-42) to form metastable low-n oligomers (LNOs) that are structurally distinct and far more cytotoxic to cultured neurons than comparable LNOs made from Aβ(1-42) alone. Tau is required for cytotoxicity, and LNOs comprising 5% Aβ(3(pE)-42) plus 95% Aβ(1-42) (5% pE-Aβ) seed new cytotoxic LNOs through multiple serial dilutions into Aβ(1-42) monomers in the absence of additional Aβ(3(pE)-42). LNOs isolated from human Alzheimer's disease brain contained Aβ(3(pE)-42), and enhanced Aβ(3(pE)-42) formation in mice triggered neuron loss and gliosis at 3 months, but not in a tau-null background. We conclude that Aβ(3(pE)-42) confers tau-dependent neuronal death and causes template-induced misfolding of Aβ(1-42) into structurally distinct LNOs that propagate by a prion-like mechanism. Our results raise the possibility that Aβ(3(pE)-42) acts similarly at a primary step in Alzheimer's disease pathogenesis.     

Bone marrow cells regenerate infarcted myocardium

Myocardial infarction leads to loss of tissue and impairment of cardiac performance. The remaining myocytes are unable to reconstitute the necrotic tissue, and the post-infarcted heart deteriorates with time. Injury to a target organ is sensed by distant stem cells, which migrate to the site of damage and undergo alternate stem cell differentiation; these events promote structural and functional repair. This high degree of stem cell plasticity prompted us to test whether dead myocardium could be restored by transplanting bone marrow cells in infarcted mice. We sorted lineage-negative (Lin-) bone marrow cells from transgenic mice expressing enhanced green fluorescent protein by fluorescence-activated cell sorting on the basis of c-kit expression. Shortly after coronary ligation, Lin- c-kitPOS cells were injected in the contracting wall bordering the infarct. Here we report that newly formed myocardium occupied 68% of the infarcted portion of the ventricle 9 days after transplanting the bone marrow cells. The developing tissue comprised proliferating myocytes and vascular structures. Our studies indicate that locally delivered bone marrow cells can generate de novo myocardium, ameliorating the outcome of coronary artery disease.     

动态应力下功率n-LDMOS器件热载流子退化恢复效应

针对功率n-LDMOS器件在动态应力条件下的热载流子退化恢复效应进行了实验和理论研究.电荷泵实验测试和器件专用计算机辅助软件的仿真分析结果表明,在施加不同的动态应力条件下,功率n-LDMOS器件存在2种主要的热载流子退化机理,即鸟嘴区域的热空穴注入和沟道区域的界面态产生,均有明显的退化恢复效应.对功率n-LDMOS器件施加2个连续3 600 s的不同应力,观察每个阶段结束时刻的CP电流曲线,发现该器件在应力变换期间确实发生了热空穴的退陷阱效应.然后,对功率n-LDMOS器件施加3个连续3 600 s的不同应力,观察每个阶段结束时刻的CP电流曲线,发现器件处于关断阶段时,已产生的界面态存在一定程度的复合.     

辽宁省生物技术产业资本市场研究

资本市场是生物技术产业发展的动力和催化剂，世界生物技术产业发展强国都在很大程度上得益于其资本市场的支持，在分析生物技术产业资本市场特点的基础上，研究辽宁省生物技术产业资本市场的现状，构建辽宁省生物技术产业的资本市场体系——对处于研究开发期阶段的企业以政府资金投入为主，对处于创业期、早期成长期和加速成长期的企业以引进风险投资为主，对处于稳定成长期和成熟期的企业以银行贷款、发行债券和上市融资为主。