Preparation of the selenomethionine derivative of tabtoxin resistance protein

In order to obtain phase information for the X-ray diffraction of tabtoxin resistance protein (TTR) crystal using the MAD phasing method, a selenomethionine (Se-Met) derivative of TTR was overexpressed in E. coli strain M15, with pQE-30 plasmid, through IPTG induction in M9 medium containing Se-Met. The product was purified to an estimated homogeneity of greater than 95% according to SDS-PAGE, by a Ni-NTA metal affinity followed by a Mono Q anion exchange column chromatography. The successful substitution of Se-Met for methionine (Met) was confirmed by MALDI-TOF and ESI-Quadrupole Mass Spectrometry analysis. The derivative crystal was obtained using similar conditions as those for the native.     

测定蛋白质与胆酸盐结合量的方法

介绍一种应用高效液相色谱仪连接示差折射率检测仪测定牛血清白蛋白与脱氧胆酸钠的结合量的简便方法．该法通过凝胶过滤实现脱氧胆酸与蛋白质的结合平衡和脱氧胆酸的分离，根据示差折射率检测仪的读数定量脱氧胆酸钠．应用该法精确测定脱氧胆酸钠与牛血清蛋白结合等温线与已报道的采用其他方法测定的结合等温线相似     

p－形式有限元法

应用微分几何语言，给计算０～３－形式物理量的ｐ－形式有限元法作了一般性的定义，并导出了１～３－形式的线性插值基函数。     

Movement and segregation of kinetochores experimentally detached from mammalian chromosomes

The kinetochore is a specialized structure at the centromere of eukaryotic chromosomes that attaches chromosomes to the mitotic spindle. Recently, several lines of evidence have suggested that kinetochores may have more than a passive role in the movement of chromosomes during mitosis and meiosis. Kinetochores seem to attract and 'capture' microtubules that grow from the spindle poles and microtubules may lengthen or shorten by the addition or subtraction of tubulin subunits at their kinetochore-associated ends. An attractive hypothesis is that kinetochores function as 'self-contained engines running on a microtubule track'. Here, we show that kinetochores can be experimentally detached from chromosomes when caffeine is applied to Chinese hamster ovary cells that are arrested in the G1/S phase of the cell cycle. The detached kinetochore fragments can still interact with spindle microtubules and complete all the mitotic movements in the absence of other chromosomal components. As these cells enter mitosis before DNA synthesis is completed, chromosome replication need not be a prerequisite for the pairing, alignment and segregation of kinetochores.     

一种动态载荷的识别方法

提出了一种逆优化系统识别动态载荷的方法。该方法避免了一般载荷识别中频响函数求逆运算中由于矩阵病态而引起的运算及运算失败，从而提高了识别精度，为动态载荷，特别是宽频带、瞬态冲击载荷的识别提供了一种有效途径。     

公路高边坡灾变机理及滑动力响应规律分析

降雨是导致和诱发滑坡的主要因素,据调查中国大约68%的滑坡灾害是由于降雨作用所致。为揭示降雨诱因与边坡岩土体劣化变形之间的关联性,以大广高速新丰段边坡为例,对降雨诱发边坡失稳破坏模式展开研究。通过建立降雨与开挖条件下的大广高速新丰段边坡的三维数值计算模型,分析滑坡应力场和位移云场演变规律,确定了滑动面埋深(H=14 m)和滑动面地表出露位置,并发现滑动面出露处呈“后缘张拉V形裂缝、舌部挤压隆起变形”为主的破坏模式,坡面以蠕滑变形为主的破坏模式;开展新丰段边坡“耦合锚索框架梁+抗滑桩”加固设计及可行性数值模拟分析;最后,在新丰段边坡上建立了公路边坡滑动力综合在线安全监测预警系统,揭示了滑动力随降雨量的动态响应规律。结果表明：在抗滑桩加固位置,坡体Z方向变形量减小,最大变形量仅为9 mm,满足边坡控制要求。可见,为类似公路高边坡的安全监测和变形机理研究奠定了理论和实践基础。     

WNT7b mediates macrophage-induced programmed cell death in patterning of the vasculature

Macrophages have a critical role in inflammatory and immune responses through their ability to recognize and engulf apoptotic cells. Here we show that macrophages initiate a cell-death programme in target cells by activating the canonical WNT pathway. We show in mice that macrophage WNT7b is a short-range paracrine signal required for WNT-pathway responses and programmed cell death in the vascular endothelial cells of the temporary hyaloid vessels of the developing eye. These findings indicate that macrophages can use WNT ligands to influence cell-fate decisions--including cell death--in adjacent cells, and raise the possibility that they do so in many different cellular contexts.     

Genome sequencing and analysis of Aspergillus oryzae

The genome of Aspergillus oryzae, a fungus important for the production of traditional fermented foods and beverages in Japan, has been sequenced. The ability to secrete large amounts of proteins and the development of a transformation system have facilitated the use of A. oryzae in modern biotechnology. Although both A. oryzae and Aspergillus flavus belong to the section Flavi of the subgenus Circumdati of Aspergillus, A. oryzae, unlike A. flavus, does not produce aflatoxin, and its long history of use in the food industry has proved its safety. Here we show that the 37-megabase (Mb) genome of A. oryzae contains 12,074 genes and is expanded by 7-9 Mb in comparison with the genomes of Aspergillus nidulans and Aspergillus fumigatus. Comparison of the three aspergilli species revealed the presence of syntenic blocks and A. oryzae-specific blocks (lacking synteny with A. nidulans and A. fumigatus) in a mosaic manner throughout the genome of A. oryzae. The blocks of A. oryzae-specific sequence are enriched for genes involved in metabolism, particularly those for the synthesis of secondary metabolites. Specific expansion of genes for secretory hydrolytic enzymes, amino acid metabolism and amino acid/sugar uptake transporters supports the idea that A. oryzae is an ideal microorganism for fermentation.     

硅基半导体中埋置硅量子点的低温生长与发光研究

在硅基半导体中埋置的硅量子点因量子限域效应而具有光致发光的性能,是一种实现硅基光电集成很有前途的材料.文章介绍了此类复合薄膜的可控生长,并对其发光进行了研究.     

紫苏微量元素的测定

利用火焰原子吸收涨研究了紫苏根、茎、叶中８种微量元素的含量,并通过实验拟定了各待测元素的最佳分析条件,呼元素的回收率在９６－１０５％之间,相对偏差小于６％,方法快速、准确、实用性强、同时,对紫苏的药理作用进行了探讨,为办的开发提供实验依据。