全文获取类型
收费全文 | 280篇 |
免费 | 0篇 |
专业分类
教育与普及 | 21篇 |
理论与方法论 | 3篇 |
现状及发展 | 11篇 |
研究方法 | 33篇 |
综合类 | 212篇 |
出版年
2012年 | 10篇 |
2011年 | 20篇 |
2010年 | 2篇 |
2009年 | 1篇 |
2008年 | 13篇 |
2007年 | 16篇 |
2006年 | 14篇 |
2005年 | 18篇 |
2004年 | 15篇 |
2003年 | 20篇 |
2002年 | 13篇 |
2001年 | 13篇 |
2000年 | 22篇 |
1999年 | 4篇 |
1998年 | 1篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 5篇 |
1993年 | 1篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1972年 | 1篇 |
1971年 | 7篇 |
1970年 | 12篇 |
1960年 | 2篇 |
1959年 | 4篇 |
1958年 | 9篇 |
1957年 | 6篇 |
1956年 | 6篇 |
1955年 | 4篇 |
1954年 | 4篇 |
1948年 | 12篇 |
排序方式: 共有280条查询结果,搜索用时 359 毫秒
211.
Gardner MJ Hall N Fung E White O Berriman M Hyman RW Carlton JM Pain A Nelson KE Bowman S Paulsen IT James K Eisen JA Rutherford K Salzberg SL Craig A Kyes S Chan MS Nene V Shallom SJ Suh B Peterson J Angiuoli S Pertea M Allen J Selengut J Haft D Mather MW Vaidya AB Martin DM Fairlamb AH Fraunholz MJ Roos DS Ralph SA McFadden GI Cummings LM Subramanian GM Mungall C Venter JC Carucci DJ Hoffman SL Newbold C Davis RW Fraser CM Barrell B 《Nature》2002,419(6906):498-511
The parasite Plasmodium falciparum is responsible for hundreds of millions of cases of malaria, and kills more than one million African children annually. Here we report an analysis of the genome sequence of P. falciparum clone 3D7. The 23-megabase nuclear genome consists of 14 chromosomes, encodes about 5,300 genes, and is the most (A + T)-rich genome sequenced to date. Genes involved in antigenic variation are concentrated in the subtelomeric regions of the chromosomes. Compared to the genomes of free-living eukaryotic microbes, the genome of this intracellular parasite encodes fewer enzymes and transporters, but a large proportion of genes are devoted to immune evasion and host-parasite interactions. Many nuclear-encoded proteins are targeted to the apicoplast, an organelle involved in fatty-acid and isoprenoid metabolism. The genome sequence provides the foundation for future studies of this organism, and is being exploited in the search for new drugs and vaccines to fight malaria. 相似文献
212.
Satellite imagery in the study and forecast of malaria 总被引:15,自引:0,他引:15
More than 30 years ago, human beings looked back from the Moon to see the magnificent spectacle of Earth-rise. The technology that put us into space has since been used to assess the damage we are doing to our natural environment and is now being harnessed to monitor and predict diseases through space and time. Satellite sensor data promise the development of early-warning systems for diseases such as malaria, which kills between 1 and 2 million people each year. 相似文献
213.
214.
本文译自《Computer Simulation Using Particles.R.W.Hockney and J.W.Eastwood.copyright c 1981.McGraw-Hill Inc.All rights reserved》一书的序言、前言和第一章。该书对粒子一网格技术现状进行了综合描述,是大学高年级学生与研究生从事粒子法计算机模拟学习的重要资料。译文追溯了计算机实验技术发展 相似文献
215.
The mouse mahogany locus encodes a transmembrane form of human attractin 总被引:16,自引:0,他引:16
Gunn TM Miller KA He L Hyman RW Davis RW Azarani A Schlossman SF Duke-Cohan JS Barsh GS 《Nature》1999,398(6723):152-156
216.
Kobayashi YM Rader EP Crawford RW Iyengar NK Thedens DR Faulkner JA Parikh SV Weiss RM Chamberlain JS Moore SA Campbell KP 《Nature》2008,456(7221):511-515
Many neuromuscular conditions are characterized by an exaggerated exercise-induced fatigue response that is disproportionate to activity level. This fatigue is not necessarily correlated with greater central or peripheral fatigue in patients, and some patients experience severe fatigue without any demonstrable somatic disease. Except in myopathies that are due to specific metabolic defects, the mechanism underlying this type of fatigue remains unknown. With no treatment available, this form of inactivity is a major determinant of disability. Here we show, using mouse models, that this exaggerated fatigue response is distinct from a loss in specific force production by muscle, and that sarcolemma-localized signalling by neuronal nitric oxide synthase (nNOS) in skeletal muscle is required to maintain activity after mild exercise. We show that nNOS-null mice do not have muscle pathology and have no loss of muscle-specific force after exercise but do display this exaggerated fatigue response to mild exercise. In mouse models of nNOS mislocalization from the sarcolemma, prolonged inactivity was only relieved by pharmacologically enhancing the cGMP signal that results from muscle nNOS activation during the nitric oxide signalling response to mild exercise. Our findings suggest that the mechanism underlying the exaggerated fatigue response to mild exercise is a lack of contraction-induced signalling from sarcolemma-localized nNOS, which decreases cGMP-mediated vasomodulation in the vessels that supply active muscle after mild exercise. Sarcolemmal nNOS staining was decreased in patient biopsies from a large number of distinct myopathies, suggesting a common mechanism of fatigue. Our results suggest that patients with an exaggerated fatigue response to mild exercise would show clinical improvement in response to treatment strategies aimed at improving exercise-induced signalling. 相似文献
217.
The genome of the model beetle and pest Tribolium castaneum 总被引:6,自引:0,他引:6
Tribolium Genome Sequencing Consortium Richards S Gibbs RA Weinstock GM Brown SJ Denell R Beeman RW Gibbs R Beeman RW Brown SJ Bucher G Friedrich M Grimmelikhuijzen CJ Klingler M Lorenzen M Richards S Roth S Schröder R Tautz D Zdobnov EM Muzny D Gibbs RA Weinstock GM Attaway T Bell S Buhay CJ Chandrabose MN Chavez D Clerk-Blankenburg KP Cree A Dao M Davis C Chacko J Dinh H Dugan-Rocha S Fowler G Garner TT Garnes J Gnirke A Hawes A Hernandez J Hines S Holder M Hume J Jhangiani SN Joshi V Khan ZM 《Nature》2008,452(7190):949-955
Tribolium castaneum is a member of the most species-rich eukaryotic order, a powerful model organism for the study of generalized insect development, and an important pest of stored agricultural products. We describe its genome sequence here. This omnivorous beetle has evolved the ability to interact with a diverse chemical environment, as shown by large expansions in odorant and gustatory receptors, as well as P450 and other detoxification enzymes. Development in Tribolium is more representative of other insects than is Drosophila, a fact reflected in gene content and function. For example, Tribolium has retained more ancestral genes involved in cell-cell communication than Drosophila, some being expressed in the growth zone crucial for axial elongation in short-germ development. Systemic RNA interference in T. castaneum functions differently from that in Caenorhabditis elegans, but nevertheless offers similar power for the elucidation of gene function and identification of targets for selective insect control. 相似文献
218.
Livet J Weissman TA Kang H Draft RW Lu J Bennis RA Sanes JR Lichtman JW 《Nature》2007,450(7166):56-62
Detailed analysis of neuronal network architecture requires the development of new methods. Here we present strategies to visualize synaptic circuits by genetically labelling neurons with multiple, distinct colours. In Brainbow transgenes, Cre/lox recombination is used to create a stochastic choice of expression between three or more fluorescent proteins (XFPs). Integration of tandem Brainbow copies in transgenic mice yielded combinatorial XFP expression, and thus many colours, thereby providing a way to distinguish adjacent neurons and visualize other cellular interactions. As a demonstration, we reconstructed hundreds of neighbouring axons and multiple synaptic contacts in one small volume of a cerebellar lobe exhibiting approximately 90 colours. The expression in some lines also allowed us to map glial territories and follow glial cells and neurons over time in vivo. The ability of the Brainbow system to label uniquely many individual cells within a population may facilitate the analysis of neuronal circuitry on a large scale. 相似文献
219.
220.
Ellinor PT Lunetta KL Albert CM Glazer NL Ritchie MD Smith AV Arking DE Müller-Nurasyid M Krijthe BP Lubitz SA Bis JC Chung MK Dörr M Ozaki K Roberts JD Smith JG Pfeufer A Sinner MF Lohman K Ding J Smith NL Smith JD Rienstra M Rice KM Van Wagoner DR Magnani JW Wakili R Clauss S Rotter JI Steinbeck G Launer LJ Davies RW Borkovich M Harris TB Lin H Völker U Völzke H Milan DJ Hofman A Boerwinkle E Chen LY Soliman EZ Voight BF Li G Chakravarti A Kubo M Tedrow UB Rose LM Ridker PM Conen D Tsunoda T 《Nature genetics》2012,44(6):670-675