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Ross P Weinhouse H Aloni Y Michaeli D Weinberger-Ohana P Mayer R Braun S de Vroom E van der Marel GA van Boom JH Benziman M 《Nature》1987,325(6101):279-281
Cellulose is the most abundant renewable carbon resource on earth and is an indispensable raw material for the wood, paper, and textile industries. A model system to study the mechanism of cellulose biogenesis is the bacterium Acetobacter xylinum which produces pure cellulose as an extracellular product. It was from this organism that in vitro preparations which possessed high levels of cellulose synthase activity were first obtained in both membranous and soluble forms. We recently demonstrated that this activity is subject to a complex multi-component regulatory system, in which the synthase is directly affected by an unusual cyclic nucleotide activator enzymatically formed from GTP, and indirectly by a Ca (2+) -sensitive phosphodiesterase which degrades the activator. The cellulose synthase activator (CSA) has now been identified as bis-(3' 5')-cyclic diguanylic acid (5'G3'p5'G3'p) on the basis of mass spectroscopic data, nuclear magnetic resonance analysis and comparison with chemically synthesized material. We also report here on intermediary steps in the synthesis and degradation of this novel circular dinucleotide, which have been integrated into a model for the regulation of cellulose synthesis. 相似文献
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Evidence from carbon isotope measurements for diverse origins of sedimentary hydrocarbons 总被引:12,自引:0,他引:12
The organic matter found in sedimentary rocks must derive from many sources; not only from ancient primary producers but also from consumers and secondary producers. In all of these organisms, isotope effects can affect the abundance and distribution of 13C in metabolites. Here, by using an improved form of a previously described technique in which the effluent of a gas chromatograph is continuously analysed isotopically, we report evidence of the diverse origins of sedimentary organic matter. The record of 13C abundances in sedimentary carbonate and total organic carbon can be interpreted in terms of variations in the global carbon cycle. Our results demonstrate, however, that isotope variations within sedimentary organic mixtures substantially exceed those observed between samples of total organic carbon. Resolution of isotope variations at the molecular level offers a new and convenient means of refining views both of localized palaeoenvironments and of control mechanisms within the global carbon cycle. 相似文献
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巨晓敏 《科技情报开发与经济》1998,(1)
在人类正朝着信息时代迈进的今天,图书馆要抓住机遇,最大限度地开发馆藏信息资源,实现资源共享并开展有图书馆特色的信息服务,在推进社会信息化进程中发挥重要作用. 相似文献
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A massive phytoplankton bloom induced by an ecosystem-scale iron fertilization experiment in the equatorial Pacific Ocean 总被引:70,自引:0,他引:70
Coale KH Johnson KS Fitzwater SE Gordon RM Tanner S Chavez FP Ferioli L Sakamoto C Rogers P Millero F Steinberg P Nightingale P Cooper D Cochlan WP Landry MR Constantinou J Rollwagen G Trasvina A Kudela R 《Nature》1996,383(6600):495-501
The seeding of an expanse of surface waters in the equatorial Pacific Ocean with low concentrations of dissolved iron triggered a massive phytoplankton bloom which consumed large quantities of carbon dioxide and nitrate that these microscopic plants cannot fully utilize under natural conditions. These and other observations provide unequivocal support for the hypothesis that phytoplankton growth in this oceanic region is limited by iron bioavailability. 相似文献
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A summary is provided of the report of an international commission set up by the University of Geneva to investigate allegations of fraud against a leading embryologist, Professor Karl Illmensee. Illmensee is being allowed to resume his faculty duties because the report, while highly critical of the researcher's experimental records, found no compelling evidence that he fabricated his data. 相似文献
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The cold dark interstellar Taurus Molecular Cloud One (TMC-1) is a rich source of acetylenic and polyacetylenic molecular species. As well as linear closed-shell molecules (H(C triple bond C)nCN) and symmetric rotors (CH3C triple bond CH, CH3C triple bond CCN), several radicals (C triple bond CH, C triple bond CCN, (C triple bond C2H) have also been identified, many of which had not been studied previously in the laboratory. Whether the observed abundances can be understood in terms of purely gas-phase ion-molecule chemical schemes, which produce reasonable agreement for the simplest polyatomic species, is unclear; alternative models involving the particulate interstellar grains as catalysts or sources have also been suggested. We now report the detection in TMC-1 of a new molecule, tricarbon monoxide (C3O), whose pure rotational spectrum has only very recently been studied in the laboratory. As C3O is the first known interstellar carbon chain molecule to contain oxygen, its existence places an important new constraint on chemical schemes for cold interstellar clouds. In fact, the observed abundance of tricarbon monoxide fits quite well into our model of galactochemistry. 相似文献
30.
Three environmental groups have filed suit against the National Institutes of Health in an attempt to block an experiment approved in April by the NIH Recombinant DNA Advisory Committee. The experiment, designed by researchers at the University of California, Berkeley, to enhance the frost resistance of crops, would release genetically engineered organisms into the general environment for the first time. The suit, filed in a federal court in the District of Columbia, claims that releasing frost-resistant bacteria into the environment could have damaging ecological consequences, that NIH should have issued an environmental impact assessment before approving any experiment involving the release of genetically engineered organisms, and that the Recombinant DNA Advisory Committee lacks the expertise to assess environmental risks. 相似文献