全文获取类型
收费全文 | 7923篇 |
免费 | 79篇 |
国内免费 | 145篇 |
专业分类
系统科学 | 126篇 |
丛书文集 | 389篇 |
教育与普及 | 274篇 |
理论与方法论 | 17篇 |
现状及发展 | 723篇 |
研究方法 | 1163篇 |
综合类 | 5441篇 |
自然研究 | 14篇 |
出版年
2018年 | 17篇 |
2017年 | 33篇 |
2016年 | 19篇 |
2015年 | 26篇 |
2014年 | 48篇 |
2013年 | 30篇 |
2012年 | 544篇 |
2011年 | 701篇 |
2010年 | 157篇 |
2009年 | 35篇 |
2008年 | 585篇 |
2007年 | 661篇 |
2006年 | 624篇 |
2005年 | 629篇 |
2004年 | 528篇 |
2003年 | 505篇 |
2002年 | 466篇 |
2001年 | 374篇 |
2000年 | 529篇 |
1999年 | 187篇 |
1998年 | 30篇 |
1997年 | 24篇 |
1996年 | 18篇 |
1995年 | 18篇 |
1994年 | 25篇 |
1993年 | 24篇 |
1992年 | 15篇 |
1991年 | 32篇 |
1990年 | 36篇 |
1989年 | 26篇 |
1988年 | 29篇 |
1987年 | 30篇 |
1986年 | 41篇 |
1985年 | 32篇 |
1984年 | 17篇 |
1983年 | 23篇 |
1982年 | 31篇 |
1981年 | 23篇 |
1980年 | 14篇 |
1979年 | 20篇 |
1971年 | 18篇 |
1970年 | 39篇 |
1966年 | 18篇 |
1959年 | 104篇 |
1958年 | 176篇 |
1957年 | 124篇 |
1956年 | 115篇 |
1955年 | 117篇 |
1954年 | 114篇 |
1948年 | 28篇 |
排序方式: 共有8147条查询结果,搜索用时 15 毫秒
41.
Mutations in the gene encoding pejvakin, a newly identified protein of the afferent auditory pathway, cause DFNB59 auditory neuropathy 总被引:5,自引:0,他引:5
Delmaghani S del Castillo FJ Michel V Leibovici M Aghaie A Ron U Van Laer L Ben-Tal N Van Camp G Weil D Langa F Lathrop M Avan P Petit C 《Nature genetics》2006,38(7):770-778
Auditory neuropathy is a particular type of hearing impairment in which neural transmission of the auditory signal is impaired, while cochlear outer hair cells remain functional. Here we report on DFNB59, a newly identified gene on chromosome 2q31.1-q31.3 mutated in four families segregating autosomal recessive auditory neuropathy. DFNB59 encodes pejvakin, a 352-residue protein. Pejvakin is a paralog of DFNA5, a protein of unknown function also involved in deafness. By immunohistofluorescence, pejvakin is detected in the cell bodies of neurons of the afferent auditory pathway. Furthermore, Dfnb59 knock-in mice, homozygous for the R183W variant identified in one DFNB59 family, show abnormal auditory brainstem responses indicative of neuronal dysfunction along the auditory pathway. Unlike previously described sensorineural deafness genes, all of which underlie cochlear cell pathologies, DFNB59 is the first human gene implicated in nonsyndromic deafness due to a neuronal defect. 相似文献
42.
Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes 总被引:56,自引:0,他引:56
Double-stranded RNA interference (RNAi) is an effective method for disrupting expression of specific genes in Caenorhabditis elegans and other organisms. Applications of this reverse-genetics tool, however, are somewhat restricted in nematodes because introduced dsRNA is not stably inherited. Another difficulty is that RNAi disruption of late-acting genes has been generally less consistent than that of embryonically expressed genes, perhaps because the concentration of dsRNA becomes lower as cellular division proceeds or as developmental time advances. In particular, some neuronally expressed genes appear refractory to dsRNA-mediated interference. We sought to extend the applicability of RNAi by in vivo expression of heritable inverted-repeat (IR) genes. We assayed the efficacy of in vivo-driven RNAi in three situations for which heritable, inducible RNAi would be advantageous: (i) production of large numbers of animals deficient for gene activities required for viability or reproduction; (ii) generation of large populations of phenocopy mutants for biochemical analysis; and (iii) effective gene inactivation in the nervous system. We report that heritable IR genes confer potent and specific gene inactivation for each of these applications. We suggest that a similar strategy might be used to test for dsRNA interference effects in higher organisms in which it is feasible to construct transgenic animals, but impossible to directly or transiently introduce high concentrations of dsRNA. 相似文献
43.
Mitochondrial dysfunction and apoptosis in myopathic mice with collagen VI deficiency 总被引:13,自引:0,他引:13
Irwin WA Bergamin N Sabatelli P Reggiani C Megighian A Merlini L Braghetta P Columbaro M Volpin D Bressan GM Bernardi P Bonaldo P 《Nature genetics》2003,35(4):367-371
Collagen VI is an extracellular matrix protein that forms a microfilamentous network in skeletal muscles and other organs. Inherited mutations in genes encoding collagen VI in humans cause two muscle diseases, Bethlem myopathy and Ullrich congenital muscular dystrophy. We previously generated collagen VI-deficient (Col6a1-/-) mice and showed that they have a muscle phenotype that strongly resembles Bethlem myopathy. The pathophysiological defects and mechanisms leading to the myopathic disorder were not known. Here we show that Col6a1-/- muscles have a loss of contractile strength associated with ultrastructural alterations of sarcoplasmic reticulum (SR) and mitochondria and spontaneous apoptosis. We found a latent mitochondrial dysfunction in myofibers of Col6a1-/- mice on incubation with the selective F1F(O)-ATPase inhibitor oligomycin, which caused mitochondrial depolarization, Ca2+ deregulation and increased apoptosis. These defects were reversible, as they could be normalized by plating Col6a1-/- myofibers on collagen VI or by addition of cyclosporin A (CsA), the inhibitor of mitochondrial permeability transition pore (PTP). Treatment of Col6a1-/- mice with CsA rescued the muscle ultrastructural defects and markedly decreased the number of apoptotic nuclei in vivo. These findings indicate that collagen VI myopathies have an unexpected mitochondrial pathogenesis that could be exploited for therapeutic intervention. 相似文献
44.
李仕成 张玉虎 周小红 柳敏良 雷祥国 郭松 高丙水 ANGELIS G MARGINEAN N GADEA A NAPOLI D R AXIOTIS M RUSU C MARTINEZ T 《中国科学(G辑)》2013,(2):135-141
利用重离子融合蒸发反应159Tb(29Si,4nγ)194Au布居了形变双奇核184Au的高自旋态,用GASP探测器阵列进行了在束γ实验测量.通过对实验数据的深入分析,新发现了一条可归属于184Au核的强耦合转动带.基于对转动带有效K值的分析以及从实验数据中提取出的带内B(M1)/B(E2)值与理论计算值的比较,建议了转动带的准粒子组态和能级的自旋宇称值. 相似文献
45.
Purification and cloning of amyloid precursor protein beta-secretase from human brain 总被引:40,自引:0,他引:40
Sinha S Anderson JP Barbour R Basi GS Caccavello R Davis D Doan M Dovey HF Frigon N Hong J Jacobson-Croak K Jewett N Keim P Knops J Lieberburg I Power M Tan H Tatsuno G Tung J Schenk D Seubert P Suomensaari SM Wang S Walker D Zhao J McConlogue L John V 《Nature》1999,402(6761):537-540
Proteolytic processing of the amyloid precursor protein (APP) generates amyloid beta (Abeta) peptide, which is thought to be causal for the pathology and subsequent cognitive decline in Alzheimer's disease. Cleavage by beta-secretase at the amino terminus of the Abeta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment. Cleavage of the C-terminal fragment by gamma-secretase(s) leads to the formation of Abeta. The pathogenic mutation K670M671-->N670L671 at the beta-secretase cleavage site in APP, which was discovered in a Swedish family with familial Alzheimer's disease, leads to increased beta-secretase cleavage of the mutant substrate. Here we describe a membrane-bound enzyme activity that cleaves full-length APP at the beta-secretase cleavage site, and find it to be the predominant beta-cleavage activity in human brain. We have purified this enzyme activity to homogeneity from human brain using a new substrate analogue inhibitor of the enzyme activity, and show that the purified enzyme has all the properties predicted for beta-secretase. Cloning and expression of the enzyme reveals that human brain beta-secretase is a new membrane-bound aspartic proteinase. 相似文献
46.
本文提出了一种利用Fabry-Perot谐振腔和介质谐振天线,同时集成偶极天线阵实现约瑟夫森结阵列自辐射的方法.在介质基片上制作集成偶极天线阵的双晶约瑟夫森结阵列,并将其嵌入到Fabry-Perot谐振腔内.将基片作为一介质谐振天线,使其与Fabry-Perot谐振腔谐振在同一频率下,从而通过高频电磁耦合机制实现结之间以及结与外部微波电路之间的耦合.文中分别进行了数值仿真和实验研究.对一个包含166个双晶约瑟夫森结的结阵列进行了液氮温度时的测试,在片外(off-chip)检测到了75.2GHz,10pW的辐射信号,与仿真结果吻合.该方法为基于约瑟夫森效应的太赫兹(THz)源提供了一种可能. 相似文献
47.
适时精确的探测土地利用和覆盖变化是研究其起因、过程和效应的基础.本研究利用人工智能神经网络对两景不同时期的影像(2003和2004年)进行一次性变化探测.并且输入层中增加了DEM和坡度两个额外"波段".在选取训练样区的过程中,共选取了82个亚类型,其中36个为植被变化亚类型,46个为无变化植被亚类型.NDVI差值法被用于探测变化和无变化的区域,该方法为获取精确的和足够的植被变化类型训练样区提供有效的参考.研究结果显示利用人工智能神经网络探测变化技术所产生的植被变化图,其精度明显高于分类后比较法所产生的植被变化图精度.此外将DEM和坡度作为额外波段结合两景影像得的8个主成分作为输入层,能够有效的提高人工智能神经网络进行山区植被变化探测精度.同时在选取训练样区的过程中,将各不同的变化和无变化的植被类型根据其不同的光谱特征分成不同的亚类型进行取样,对提高人工智能神经网络进行山区植被变化探测精度有着非常重要的促进作用,此处理可以降低山区影像的地形效应. 相似文献
48.
49.
Persistence of soil organic matter as an ecosystem property 总被引:65,自引:0,他引:65
Schmidt MW Torn MS Abiven S Dittmar T Guggenberger G Janssens IA Kleber M Kögel-Knabner I Lehmann J Manning DA Nannipieri P Rasse DP Weiner S Trumbore SE 《Nature》2011,478(7367):49-56
Globally, soil organic matter (SOM) contains more than three times as much carbon as either the atmosphere or terrestrial vegetation. Yet it remains largely unknown why some SOM persists for millennia whereas other SOM decomposes readily--and this limits our ability to predict how soils will respond to climate change. Recent analytical and experimental advances have demonstrated that molecular structure alone does not control SOM stability: in fact, environmental and biological controls predominate. Here we propose ways to include this understanding in a new generation of experiments and soil carbon models, thereby improving predictions of the SOM response to global warming. 相似文献
50.
Thyristors are a class of nonlinear electronic device that exhibit bistable resistance--that is, they can be switched between two different conductance states. Thyristors are widely used as inverters (direct to alternating current converters) and for the smooth control of power in a variety of applications such as motors and refrigerators. Materials and structures that exhibit nonlinear resistance of this sort are not only useful for practical applications: they also provide systems for exploring fundamental aspects of solid-state and statistical physics. Here we report the discovery of a giant nonlinear resistance effect in the conducting organic salt theta-(BEDT-TTF)2CsCo(SCN)4, the voltage-current characteristics of which are essentially the same as those of a conventional thyristor. This intrinsic organic thyristor works as an inverter, generating an alternating current when a static direct-current voltage is applied. Whereas conventional thyristors consist of a series of diodes (their nonlinearity comes from interface effects at the p-n junctions), the present salt exhibits giant nonlinear resistance as a bulk phenomenon. We attribute the origin of this effect to the current-induced melting of insulating charge-order domains, an intrinsically non-equilibrium phenomenon in the sense that ordered domains are melted by a steady flow. 相似文献