全文获取类型
收费全文 | 145篇 |
免费 | 2篇 |
专业分类
系统科学 | 2篇 |
教育与普及 | 2篇 |
理论与方法论 | 1篇 |
现状及发展 | 40篇 |
研究方法 | 22篇 |
综合类 | 80篇 |
出版年
2018年 | 1篇 |
2017年 | 3篇 |
2016年 | 2篇 |
2015年 | 8篇 |
2014年 | 2篇 |
2013年 | 10篇 |
2012年 | 11篇 |
2011年 | 11篇 |
2010年 | 1篇 |
2009年 | 1篇 |
2008年 | 10篇 |
2007年 | 8篇 |
2006年 | 14篇 |
2005年 | 11篇 |
2004年 | 11篇 |
2003年 | 8篇 |
2002年 | 8篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1994年 | 2篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1975年 | 2篇 |
1974年 | 1篇 |
1970年 | 2篇 |
1969年 | 1篇 |
1967年 | 1篇 |
1966年 | 2篇 |
1960年 | 1篇 |
排序方式: 共有147条查询结果,搜索用时 218 毫秒
41.
Senderek J Krieger M Stendel C Bergmann C Moser M Breitbach-Faller N Rudnik-Schöneborn S Blaschek A Wolf NI Harting I North K Smith J Muntoni F Brockington M Quijano-Roy S Renault F Herrmann R Hendershot LM Schröder JM Lochmüller H Topaloglu H Voit T Weis J Ebinger F Zerres K 《Nature genetics》2005,37(12):1312-1314
SIL1 (also called BAP) acts as a nucleotide exchange factor for the Hsp70 chaperone BiP (also called GRP78), which is a key regulator of the main functions of the endoplasmic reticulum. We found nine distinct mutations that would disrupt the SIL1 protein in individuals with Marinesco-Sj?gren syndrome, an autosomal recessive cerebellar ataxia complicated by cataracts, developmental delay and myopathy. Identification of SIL1 mutations implicates Marinesco-Sj?gren syndrome as a disease of endoplasmic reticulum dysfunction and suggests a role for this organelle in multisystem disorders. 相似文献
42.
43.
44.
TNF-mediated inflammatory skin disease in mice with epidermis-specific deletion of IKK2 总被引:31,自引:0,他引:31
Pasparakis M Courtois G Hafner M Schmidt-Supprian M Nenci A Toksoy A Krampert M Goebeler M Gillitzer R Israel A Krieg T Rajewsky K Haase I 《Nature》2002,417(6891):861-866
The I kappa B kinase (IKK), consisting of the IKK1 and IKK2 catalytic subunits and the NEMO (also known as IKK gamma) regulatory subunit, phosphorylates I kappa B proteins, targeting them for degradation and thus inducing activation of NF-kappa B (reviewed in refs 1, 2). IKK2 and NEMO are necessary for NF-kappa B activation through pro-inflammatory signals. IKK1 seems to be dispensable for this function but controls epidermal differentiation independently of NF-kappa B. Previous studies suggested that NF-kappa B has a function in the growth regulation of epidermal keratinocytes. Mice lacking RelB or I kappa B alpha, as well as both mice and humans with heterozygous NEMO mutations, develop skin lesions. However, the function of NF-kappa B in the epidermis remains unclear. Here we used Cre/loxP-mediated gene targeting to investigate the function of IKK2 specifically in epidermal keratinocytes. IKK2 deficiency inhibits NF-kappa B activation, but does not lead to cell-autonomous hyperproliferation or impaired differentiation of keratinocytes. Mice with epidermis-specific deletion of IKK2 develop a severe inflammatory skin disease, which is caused by a tumour necrosis factor-mediated, alpha beta T-cell-independent inflammatory response that develops in the skin shortly after birth. Our results suggest that the critical function of IKK2-mediated NF-kappa B activity in epidermal keratinocytes is to regulate mechanisms that maintain the immune homeostasis of the skin. 相似文献
45.
This paper provides an account of early historical developments in the characterization and quantification of acidity, which may be considered preliminary steps leading to the measurement of acidity. In this “pre-history” of acidity measurement, emphasis is laid on the relative independence of the rich empirical knowledge about acids from theories of acidity. Many attempts were made to compare and assess the strengths of various acids, based on concrete laboratory operations. However, at least until the arrival of the pH measure, the quantification attempts failed to produce anything qualifying as a measurement scale of a recognizable type. It is doubtful whether even pH qualifies as a true measure of acidity, when the full meaning of acidity is taken into account. 相似文献
46.
Recycling of oceanic crust through subduction, mantle upwelling, and remelting in mantle plumes is a widely accepted mechanism to explain ocean island volcanism. The timescale of this recycling is important to our understanding of mantle circulation rates. Correlations of uranogenic lead isotopes in lavas from ocean islands such as Hawaii or Iceland, when interpreted as model isochrons, have yielded source differentiation ages between 1 and 2.5?billion years (Gyr). However, if such correlations are produced by mixing of unrelated mantle components they will have no direct age significance. Re-Os decay model ages take into account the mixing of sources with different histories, but they depend on the assumed initial Re/Os ratio of the subducted crust, which is poorly constrained because of the high mobility of rhenium during subduction. Here we report the first data on (87)Sr/(86)Sr ratios for 138 melt inclusions in olivine phenocrysts from lavas of Mauna Loa shield volcano, Hawaii, indicating enormous mantle source heterogeneity. We show that highly radiogenic strontium in severely rubidium-depleted melt inclusions matches the isotopic composition of 200-650-Myr-old sea water. We infer that such sea water must have contaminated the Mauna Loa source rock, before subduction, imparting a unique 'time stamp' on this source. Small amounts of seawater-derived strontium in plume sources may be common but can be identified clearly only in ultra-depleted melts originating from generally highly (incompatible-element) depleted source components. The presence of 200-650-Myr-old oceanic crust in the source of Hawaiian lavas implies a timescale of general mantle circulation with an average rate of about 2 (±1)?cm?yr(-1), much faster than previously thought. 相似文献
47.
Najmabadi H Hu H Garshasbi M Zemojtel T Abedini SS Chen W Hosseini M Behjati F Haas S Jamali P Zecha A Mohseni M Püttmann L Vahid LN Jensen C Moheb LA Bienek M Larti F Mueller I Weissmann R Darvish H Wrogemann K Hadavi V Lipkowitz B Esmaeeli-Nieh S Wieczorek D Kariminejad R Firouzabadi SG Cohen M Fattahi Z Rost I Mojahedi F Hertzberg C Dehghan A Rajab A Banavandi MJ Hoffer J Falah M Musante L Kalscheuer V Ullmann R Kuss AW Tzschach A Kahrizi K Ropers HH 《Nature》2011,478(7367):57-63
48.
Klaus Brockhoff 《Journal of forecasting》1984,3(4):417-428
It is assumed that demand for information that subjectively appears to be relevant for forecasting improves forecasting quality. To study this hypothesis a number of forecasting experiments were conducted. Fifty managers from the housing business, from banking, and from a research institution were asked to forecast interest rates, using a Delphi process. They communicated via a computer system, and, to support their judgements, they had access to a data bank that was stored in the same system. Their communication with the system was automatically recorded. Part of the data collected in these experiments is used to study the existence of a relationship between information activities and forecasting results. A weak positive relationship is found if non-linear functions are tested, where information demand is corrected by those data retrievals that seem to have resulted from an inability to handle the information system. For further research a more general, albeit less informative, Boolean model is suggested. 相似文献
49.
Bowler C Allen AE Badger JH Grimwood J Jabbari K Kuo A Maheswari U Martens C Maumus F Otillar RP Rayko E Salamov A Vandepoele K Beszteri B Gruber A Heijde M Katinka M Mock T Valentin K Verret F Berges JA Brownlee C Cadoret JP Chiovitti A Choi CJ Coesel S De Martino A Detter JC Durkin C Falciatore A Fournet J Haruta M Huysman MJ Jenkins BD Jiroutova K Jorgensen RE Joubert Y Kaplan A Kröger N Kroth PG La Roche J Lindquist E Lommer M Martin-Jézéquel V Lopez PJ Lucas S Mangogna M McGinnis K 《Nature》2008,456(7219):239-244
Diatoms are photosynthetic secondary endosymbionts found throughout marine and freshwater environments, and are believed to be responsible for around one-fifth of the primary productivity on Earth. The genome sequence of the marine centric diatom Thalassiosira pseudonana was recently reported, revealing a wealth of information about diatom biology. Here we report the complete genome sequence of the pennate diatom Phaeodactylum tricornutum and compare it with that of T. pseudonana to clarify evolutionary origins, functional significance and ubiquity of these features throughout diatoms. In spite of the fact that the pennate and centric lineages have only been diverging for 90 million years, their genome structures are dramatically different and a substantial fraction of genes ( approximately 40%) are not shared by these representatives of the two lineages. Analysis of molecular divergence compared with yeasts and metazoans reveals rapid rates of gene diversification in diatoms. Contributing factors include selective gene family expansions, differential losses and gains of genes and introns, and differential mobilization of transposable elements. Most significantly, we document the presence of hundreds of genes from bacteria. More than 300 of these gene transfers are found in both diatoms, attesting to their ancient origins, and many are likely to provide novel possibilities for metabolite management and for perception of environmental signals. These findings go a long way towards explaining the incredible diversity and success of the diatoms in contemporary oceans. 相似文献
50.
Scheerer P Park JH Hildebrand PW Kim YJ Krauss N Choe HW Hofmann KP Ernst OP 《Nature》2008,455(7212):497-502
Opsin, the ligand-free form of the G-protein-coupled receptor rhodopsin, at low pH adopts a conformationally distinct, active G-protein-binding state known as Ops*. A synthetic peptide derived from the main binding site of the heterotrimeric G protein-the carboxy terminus of the alpha-subunit (GalphaCT)-stabilizes Ops*. Here we present the 3.2 A crystal structure of the bovine Ops*-GalphaCT peptide complex. GalphaCT binds to a site in opsin that is opened by an outward tilt of transmembrane helix (TM) 6, a pairing of TM5 and TM6, and a restructured TM7-helix 8 kink. Contacts along the inner surface of TM5 and TM6 induce an alpha-helical conformation in GalphaCT with a C-terminal reverse turn. Main-chain carbonyl groups in the reverse turn constitute the centre of a hydrogen-bonded network, which links the two receptor regions containing the conserved E(D)RY and NPxxY(x)(5,6)F motifs. On the basis of the Ops*-GalphaCT structure and known conformational changes in Galpha, we discuss signal transfer from the receptor to the G protein nucleotide-binding site. 相似文献